Functional Activities of Microwave-Assisted Extracts from Lyophyllum ulmarium

마이크로웨이브 추출공정에 의한 만가닥버섯의 기능적 특성

  • Published : 2002.12.01

Abstract

Functional activities of Lyophyllum ulmarium microwave-assisted extracts under different conditions including electron donating ability, tyrosinase inhibition activity and nitrite scavenging effect were examined. Total polyphenol content increased as increasing microwave power up to 90 W in the water extracts. Electron donating ability increased with microwave power up to 90 W in 50% ethanol extract and 99% ethanol extract. Tyrosinase inhibition activity and nitrite scavenging effect in the extract increased as microwave power increased during extraction. Total polyphenol content increased as extending extraction time up to 5 min in the water extract. But the highest electron donating ability and tyrosinase inhibition activity was obtained after 10 min extraction. Significantly higher total polyphenol content and electron donating ability were found in the water extract whereas greater tyrosinase inhibition activity and nitrite scavenging effect were observed in 99% ethanol extract. The maxium nitrite scavenging effect was found at pH 1.2 and decreased as pH increased.

마이크로웨이브 추출(MAE)을 이용하여 만가닥버섯을 물, 50% 에탄올, 99% 에탄올로 추출하였으며 마이크로웨이브의 power와 시간에 따라 추출물의 총 폴리페놀 함량, 전자공여 작용(EDA), tyrosinase 저해작용, 아질산염 소거작용의 차이를 관찰하였다. Microwave power를 60∼120 W로 증가시킴에 따라 총 폴리페놀 함량과 전자공여능은 증가하는 경향을 나타내었다. 만가닥버섯의 전자공여능이 90 W에서 평형에 이른 반면에 tyrosinase 저해능은 90 W 이상에서 급격히 증가하였다. 50% 에탄을 추출물과 99% 에탄을 추출물의 아질산염 소거능은 microwave power를 증가시킴에 따라 확연히 증가하는 경향을 나타내었으며 pH 1.2에서 99% 에탄을 추출물은 물 추출물에 비해 약 5배 정도의 높은 값을 나타내었다. 추출시간에 따른 총 폴리페놀 함량을 살펴본 결과 물 추출물의 경우 추출시간 5분에서 평형에 이르렀다. 전자공 여능은 5∼10분 사이에 급격히 증가하다가 10분에서 평형에 이르렀으며 물 추출물에서 가장 높게 나타내었다. tyrosinase 저해능은 microwave power 증가에 따라 점진적으로 증가하는 경향을 나타내었다.

Keywords

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