Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Study on isolation of Prevotella nigrescens 9336- specific DNA probes using random cloning method

무작위 클로닝법을 이용한 Prevotella nigrescens 9336 특이 DNA 프로브의 개발에 관한 연구

  • Gang, Soon-Won (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Se-Hoon (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • kim, Dong- Ki (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Seong, Jin-Hyo (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Jung- Ki (Dept. of Oral Biochemistry, College of Dentistry, Chosun University)
  • 강순원 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 김세훈 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 김동기 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 성진효 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 김병옥 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실)
  • Published : 2002.06.30
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Abstract

The purpose of this study is to develop species-specific DNA probes and polymerase chain reaction (PCR) primers for detection and identification of Prevotella nigrescens (P. nigrescens) 9336. This study procedure includes (1) whole-genomic DNA extraction of P. nigrescens 9336 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse Dot Hybridization method, (4) confirmation of strain-specific DNA probe by Southern blot analysis, (5) determination of nucleotide sequences of strain-specific DNA probe. Thirty-five restriction fragments of P. nigrescens 9336 genomic DNA digested with the Hind III were obtained. Reverse dot hybridization and Southern blot analysis data showed that three of them, Pn10, Pn23, and Pn35, could be P. nigrescens 9336-specific DNA probes. These data indicated that these DNA probes could be useful in detection and identification of the P. nigrescens 9336.

Keywords

References

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