Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Study on development of DNA probe for identification of Prevotella intermedia G8-9-3

Prevotella intermedia G8-9K-3을 동정할 수 있는 DNA 프로브의 개발에 관한 연구

  • Bak, Jong-Sung (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Se-Hoon (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • kim, Dong-Ki (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Seong, Jin-Hyo (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Dept. of Periodontology, Dept. of preventive Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Jung-Ki (Dept. of Oral Biochemistry, College of Dentistry, Chosun University)
  • 백종성 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 김세훈 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 김동기 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 성진효 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 김병옥 (조선대학교 치과대학 치주과학교실, 조선대학교 치과대학 예방치과학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실)
  • Published : 2002.06.30
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Abstract

The purpose of this study is to develop species-specific DNA probe for detection and identification of Prevotella intermedia (P. intermedia) G8-9K-3. This study procedure includes (1) whole-genomic DNA extraction of P. intermedia G8-9K-3 (2) construction of the genomic DNA library, (3) screening of strain-specific DNA probe by reverse dot hybridization, (4) confirmation of strain-specific DNA probe by Southern blot hybridization, (5) determination of nucleotide sequences of strain-specific DNA probe. Twenty-eight recombinant plasmids containing Hind III-digested DNA fragments of P. intermedia G8-9K-3 were obtained. Reverse Dot Hybridization and Southern blot analysis data showed that one of them, Pig3, could be P. intermedia G8-9K-3-specific DNA probe. This datum indicates that this Pig3 DNA probe could be useful in detection and identification of the P. intermedia G8-9K-3 strain.

Keywords

References

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