Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

The Effect of Bee Venom & Purified Bee Venom on Cell Death in Synovial Cell

국산(國産) 봉독(蜂毒) 및 정제(精製) 봉독약침액(蜂毒藥鍼液)이 류머티스 관절염(關節炎) 활액세포(滑液細胞)에 미치는 영향(影響)

  • Lee, Yun-Seop (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Lee, Seung-Woo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Han, Sang-Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University)
  • 이윤섭 (경산대학교 한의과대학 침구학교실) ;
  • 서정철 (경산대학교 한의과대학 침구학교실) ;
  • 이승우 (경산대학교 한의과대학 침구학교실) ;
  • 한상원 (경산대학교 한의과대학 침구학교실)
  • Received : 2002.03.07
  • Accepted : 2002.03.19
  • Published : 2002.04.20
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study is aimed to investigate the effects of bee venom and purified bee venom on cell death in synovial cell line. Methods : It was evaluated by using MTT assay, morphological method, flow cytometry, immunocytochemistry analysis, RT-PCR. Results : The result obtained is as follows. 1. The MTT assay demonstrated that synovial cell viability was significantly inhibitted dose-dependently by treatment with BV and PBV in comparison with control. And the inhibitory effect of BV and PBV was almost same. 2. The morphologic study demonstrated that synovial cell showed apoptotic body resulted from apoptosis after treatment with BV and PBV for 6 hours using microscope. 3. The Flow cytometry demonstrated that apoptosis of synovial cell treated with BV and PBV was related with stop of cell cycle in stage of G0/G1. 4. Immunocytochemistry assay demonstrated that COX-II and iNOS were slightly expressed by treatment with BV and PBV in comparison with control group. 5. RT-PCR analysis demonstrated that COX-II were almost down-regulated by high dose treatment with BV and PBV in comparison with control group. iNOS were well down-regulated by treatment with $5{\mu}g/ml$ BV and PBV whereas it was well expressed in control group. Conclusion : These results suggest that bee venom and purified bee venom have significant effect on cell death in synovial cell line and further study is needed in vivo.

Keywords