Studies on the mycoplasmal pneumonia in slaughter pigs. 1. Seasonal detection by gross finding of lung lesion and dot-ELISA technique

도축돈의 마이코플라즈마성 폐렴에 관한 연구 1. 육안적 폐병변과 dot-ELISA에 의한 계절별 조사

  • Lim, Young-Taek (Department of Animal Science, College of life Sciences, Dankook University) ;
  • Seok, Ho-Bong (Department of Animal Science, College of life Sciences, Dankook University)
  • 임영택 (단국대학교 생명자원과학부 동물자원 전공) ;
  • 석호봉 (단국대학교 생명자원과학부 동물자원 전공)
  • Accepted : 2002.04.24
  • Published : 2002.06.29

Abstract

We report the seasonal prevalence of the mycoplasmal pneumoniae of swine (MPS) in slaughter pigs from July of 1999 to June of 2000. Gross finding of lung lesion observed and examined by dot-ELISA. In gross finding of lung lesion from 750 pig samples, 465 (62.0%) was MPS, and 129 (17.2%) was single or double infection with actinobacillosis and pasturellosis. However, 156 (20.8%) had no lesion. In seasonal detection, the prevalence was found to be winter (69.5%), autumn (63.5%), summer (60.0%) and spring (54.7%) in orderly frequency. In dot-ELISA, the result was showed the positive reaction (x16>titre) with 58.0% and negative (x4

Keywords

References

  1. Ross RF. Mycoplasmal disease. ln:Leman AD, Straw BE, Mangeling et al ; Diseses of Swine, 7th ed. Ames, Iowa : Iowa State University Press, 537-551, 1992
  2. Maes D, Verdonck M, Deluyker H et al. Enzootic pneumoniae in pigs. Vet. Q. 18, 104-109, 1996
  3. Weisbmg WG, Tully JG, Rose DL et al. A phylogenetic analysis of the Mycoaplasmas : Basis for their classification. J. Bacteriol. 171, 6455-6467, 1989
  4. Tully JG, Bove JM, Laigret F et al. Revised taxonomy of the class ordinal rank(Entomoplasmatales, ord. nov.), with provision for familial rank to separate species with nonhelical morphology from helical species, and emended descriptions of the order Mycoplasmatales, family My coplasmataceae. Int. J. Syst. Bacteriol. 43, 378-385, 1993
  5. Seok HB, Joo HS. Investigation of seroepidemiology of Mycoplasma hycpeumoinae infection and establishment of on-farm eradication protocol. Kor. J. yet, Res 39(6): 1218.1223, 1999
  6. Pullar EM. Infectious pneumoniae of pigs. General description, differential diagnosis and epidemiology. Aust. Vet. J. 24, 320-330, 1948
  7. Livingston CW Jr, Stair EL, Undardahl NR et al. Pathogenesis of mycoplasmal pneumoniae in swine. Am.J.Vet.Kes. 33:2249-2258, 1972
  8. Amanfu W, Weng CN, Ross RF at al. Diagnosis of mycoplasmal pneumoniae of swine : Sequential study by direct immunofluorence. Am. J. Vet. Res. 45, 1349-1352, 1984
  9. Amstrong CH, Freeman MJ, Sands-Freeman L et al. Comparison of enzyme-linked immunosorbent assay and the indirect hemmagglutination nad complement fixation tests for detecting antibodies to Mycoplasma hyopneumoniae. Can. J. Comp. Med 47, 464-470, 1983
  10. Ross RF, Whittlestone P. Recovery, identification of, and serological response to porcine mycoplasmas. In: Tully JG, Razin S Methods in Mycoplasmology, Vol. 2. New York: Academic Press, 1983
  11. Piffer IA, Ross RF. Effect of age on susceptibility of pigs to Mycoplasma hyopneumoniae. Am. J. Vet. Res. 45, 478-481 1984
  12. Nicolet J, Paroz P, Bruggmann S. Tween 20 soluble proteins of Mycoplasma hyopneumoniae as antigen for an enzyme-linked immunosorbent assay. Res. Vet. Sci. 29, 305-309, 1980
  13. Le Potier MF, Abiven P, Kobisch M. A blocking ELISA using a manoclonal antibody for the serological detection of Mycoplasma hyopneumoniae. Ann. Rech. Vet. 23, 239-247, 1994
  14. Kotani H, Huang H, McGarrity GJ. Identification and isolation of mycoplasmas by immunobinding. Israel J. of Med 23:752-758, 1987
  15. Charles SD, Sreevatsan S, Bey RF et al. A dot immnuobinding assay(dot-ELISA)for the rapid sero-diagnosis of Salmonella enterutidis infection in chickens. J. Vet. Diagn. Invest, 8:310-314,1996
  16. Afshar A, Wright PF, Dulac GC. Dot-enzyme immunoassay for visual detection of antibodies to pseudorabies virus in swine serum. J of Clinical Microbiol, 23(3):563-567, 1986
  17. Friis NF, Kobisch M. Swine mycoplasmoses. Rev. Sci. Tech. Off. Int. Epiz. 15(4), 1569-1605, 1996
  18. Straw BE, Backstrom L, Leman AD. Examination of swine at slaughter. Part II. Finding at slaughter and their significance. Compend Contin. Educ. Pract. Vet, 8:S106-S112, 1986
  19. Morrison PB, Pijoan C, Hilley HD. Microorganisms associated with pneumonia in slaughter weight swine. Can. J. Comp. Med. 49, 129-137, 1985
  20. Robert EA, Rocky ST. Ultrafast protein determinations using microwave enhancement. Molecular Biotechnology. Himanna Press. Inc. 17-24, 1995
  21. Mariano Z, Charles LJ. Routine dot-blot assay of multiple serum samples using a simple apparatus. J. Immunological Methods. 101, 261-264, 1987
  22. 조광현, 최정수, 김봉환. 영남지방 도축돈의 Mycoplasma 폐렴조사 및 분리균에 대한 약제 감수성. 대한수의학회지. 39(1), 96-103,1999