Hydrogen and Organic Acids Production by Fermentation Using Various Anaerobic Bacteria

각종 혐기성 미생물 발효에 의한 유기산 및 수소생산

  • Kim, Mi-Sun (Biomass Research Team Korea Institute of Energy Research) ;
  • Yoon, Y.S. (Biomass Research Team Korea Institute of Energy Research) ;
  • Sim, S.J. (School of Chemical Engineering, SungKyunKwan Univ .) ;
  • Park, T.H. (Seoul National Univ.) ;
  • Lee, J.K. (Sogang Univ.)
  • 김미선 (한국에너지기술연구원 바이오매스 연구팀) ;
  • 윤영수 (한국에너지기술연구원 바이오매스 연구팀) ;
  • 심상준 (성균관대학교 화학공학과) ;
  • 박태현 (서울대학교 응용화학부) ;
  • 이정국 (서강대학교 생명과학과)
  • Published : 2002.12.15

Abstract

Clostridium butyricum, Lactobacillus amylophillus, Lactobacillus amylovorus, Lactobacillus acidophillus, AI-9 produced hydrogen and /or organic acids using glucose, lactose and starch at the anaerobic culture conditions. Cl. butyricum NCIB 9576 evolved 1,700 ml H2/L-culture broth and accumulated butyric acid, acetic acid, propionic acid and ethanol in its culture broth when lactose was used as a carbon source during 24 hrs of fermentation. L. amylovorus ATCC 33620 accumulated lactic and acetic acids and some reducing sugars when starch was used as a carbon source without hydrogen production. Instead of starch as a carbon source, L. amylovorus ATCC 33620 produced lactic acid from algal biomass during fermentation and the acid-heat or freeze-thaw pretreatment of algal biomass accelerate the lactic acid fermentation.

Keywords

Acknowledgement

Supported by : 과학기술부

References

  1. J. G. Van Andel, G. R. Zoutberg, P. M. Crabbendam, and A. M. Breure : "Glucose fermentation by Clostridium butyricum grown under a self generated gas atmosphere in chemostat culture", Applied Microbiology and Biotechnology, Vol. 23, 1985, pp. 21-26 https://doi.org/10.1007/BF02660113
  2. M.. Heyndickx, P. De Vos, B. Thibau, P. Stevens, J. I. De Ley, : "Hydrogen Gas Production from Continuous Fermentation of Glucose in a Minimal Medium with Clostridium butyricum", System. Applied Microbiology, 1986, Vol. 8, pp. 239-244 https://doi.org/10.1016/S0723-2020(86)80087-X
  3. P. M. Crabbendam, O. M. Neijssel, and D. W. Tempest : "Metabolic and Energetic Aspcets of the Growth of Clostridium butyricum on Glucose in Chemostat Culture", Archives of Microbiology, 1985, Vol. 142, pp. 375-382 https://doi.org/10.1007/BF00491907
  4. D. X. Zhang, and M. Cheryan : "Direct Fermentation of Starch to Lactic Acid byLactobacillus amylovorus", Biotechnology Letters, Vol. 13, No. 10, 1991, pp. 733-738 https://doi.org/10.1007/BF01088178
  5. A. Ike, N. Toda, T. Murakawa, K. Hirata, and K. Miyamoto : in Biohydrogen, Zaborsky (Edi.) Plenum Press New York pp. 311-318
  6. H. Kawaguchi, K. Hashimoto, K. Hirata, and K. Miyamoto : "$H_2$ production from Algae Biomass by a Mixed Culture of Rhodobium marinum A-501 and Lactobacillus amylovorus", Journal of Bioscience and Bioeng.eneering, Vol. 91, No. 3, 2001, pp. 277-282 https://doi.org/10.1263/jbb.91.277
  7. M.. Heyndickx, P. De Vos, B. Thibau, P. Stevens, J. I. De Ley, : "Effect of Various External Factors on the Fermentative Production of Hydrogen Gas from Glucose by Clostridium butyricum Strains in Batch Culture", System. Applied Microbiology, 1987, Vol. 9, PP. 163-168 https://doi.org/10.1016/S0723-2020(87)80072-3