Empirical Evaluation of Cellulase on Enzymatic Hydrolysis of Waste Office Paper

  • Park, Enoch Y. (Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty Agriculture, Shizuoka University) ;
  • Ikeda, Yuko (Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty Agriculture, Shizuoka University) ;
  • Okuda, Naoyuki (Technical Developments Department, Tsukishima Kikai Co. Ltd.)
  • Published : 2002.09.01

Abstract

Enzymatic hydrolysis of waste office paper was evaluated using three commercial cellulases, Acremonium cellulase, Meicelase, and Cellulosin T2. Varying the enzyme loading from 1 to 10% (w/w) conversion of waste office paper to reducing sugar was investigated. The conversion increased with the increase in the enzyme loading: in the case of enzyme loading of 10% (w/w), Acremonium cellulase yielded 79%conversion of waste office paper, which was 17% higher compared to Meicelase, 13% higher than that of Cellulosin T2. Empirical model for the conversion (%) of waste office paper to re-ducing sugar (x) was derived from experimental results as follow, x = $kE^{m}t^{(aE+b)}$ where k, m, a, and b de-note empirical constants. E indicates initial enzyme concentration.

Keywords

References

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