The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effect of Sohaphyang-won's for Delayed Neuronal Death in Hypoxia

소합향원(蘇合香元)이 저산소증 유발 배양 대뇌신경세포에 미치는 영향

  • Yun Kyoung-Sun (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jeong Sung-Hyun (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Shin Gil-Cho (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Lee Won-Chu (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Moon Il-Su (Department of Anatomy, College of Medicine, Dongguk University*) ;
  • Lee Ji-Hun (Department of Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 윤경선 (동국대학교 한의과대학 내과학교실) ;
  • 정승현 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실) ;
  • 이원철 (동국대학교 한의과대학 내과학교실) ;
  • 문일수 (동국대학교 해부학교실) ;
  • 이지훈 (동국대학교 한의과대학 내과학교실)
  • Published : 2003.03.01
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Abstract

Objectives : The purpose of this study is to evaluate the effects of Sohaphyang-won and is to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20(E20) cortical cells of a guinea pig(Sprague Dawley). Methods : E20 cortical cells, used in this investigation were dissociated in Neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Sohaphyang-won was added to the culture media for 72 hours. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Sohaphyang-won's effects for neuronal death protection were evaluated by LDH assay and the mechanism was studied by Bcl-2, Bak, Bax, caspase family. Results : This study indicates that Sohaphyang-won's effects for neuronal death protection in hypoxia is confirmed by LDH assay by the method of Embryonic day 20(E20) cortical neuroblast. Conclusions : Sohaphyang-won's mechanism for neuronal death protection in hypoxia restrains inflow of cytochrome C into cellularity caused by Bcl-2 increase and reduces the caspase cascade initiator caspase-10 and the effector caspase-3.

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