Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Grape Seed Extract Protects Mice against Disseminated Candidiasis

  • Published : 2003.06.01
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Abstract

Effect of grape seed extract (GSE) against Candida albicans was examined under in-vitro and in-vivo conditions. The GSE was extracted in ethanol. In-vitro results from an agar diffusion susceptibility assay showed the GSE inhibited C. albicans growth. This anticandidal effect was at dose-dependency. In experiments with animals, mice that received the GSE (0.5 mg per mice), intravenously (i.v.), before i.v.-infection wish viable C. albicans yeast cells survived longer than diluent (buffer)-received control mice. In contrast, when GSE was given to mice after the mice were infected with the yeast cells, these mice showed a similar survival rate as compared to control mice that received no treatment with the GSE. Taken together, these data indicate that GSE has prophylactic effect but not therapeutic effect against disseminated candidiasis.

Keywords

References

  1. Body, G. P., 1988. The emergence of fungi as major hospital pathogens. J. Hosp. Infect. 11(Suppl A): 411-26 https://doi.org/10.1016/0195-6701(88)90220-4
  2. Edwards, I E., 1991. Invasive Candida infections: evolution of a fungal pathogen. N. Engl. J. Med. 324: 1060-2 https://doi.org/10.1056/NEJM199104113241511
  3. Han, Y, Cutler, J. E., 1995. Antibody response that protects against disseminated candidasis. Infect. Immun. 63: 2714-2719
  4. Han, Y, Riesselman, M. H., Cutler, J. E., 2000. Protection against candidiasis by an immunoglobulin G3 (IgG3) monoclonal antibody specific for the same mannotriose as an IgM protective antibody. Infect. Immun. 68: 1649-1654 https://doi.org/10.1128/IAI.68.3.1649-1654.2000
  5. Han, Y, Ulrich, M. A., Cutler, J. E., 1999. Candida albicans mannan extract protein conjugates induce a protective immune response against experimental candidiasis. J. Infect. Dis. 179: 1477-84 https://doi.org/10.1086/314779
  6. Han, Y., Kozel, T. R., Zhang, M. x., MacGill, R. S., Carroll, M. c., Cutler, I E., 2001. Complement is essential for protection by an IgM and an IgG3 monoclonal antibody against experimental, hematogenously disseminated candidiasis. J. Immun. 167: 1550-1557 https://doi.org/10.4049/jimmunol.167.3.1550
  7. Koga, T., Moro, K., Nakamori, K., Yamakoshi, J., Hosoyama, H., Kataoka, S., Ariga, T., 1999. Increase of antioxidative potential of rat plasma by oral administration of proanthocyanidin-rich extract form grape seeds. J Agric Food Chem. 47(5): 1892-7 https://doi.org/10.1021/jf9810517
  8. Maffei, F. R., Carini, M., Aldini, G., Berti, F., Rossoni, G., Bombardelli, E., Morazzoni, P.. 1996. Procyanidines from Vitis vinifera seeds protect rabbit heart from ischemia/reper fusion injury; antioxidant intervention and/or iron and copper sequestering ability. Planta. Med. 62(6): 495-502 https://doi.org/10.1055/s-2006-957956
  9. Nair, N., Mahajan, S., Chawda, R., Kandaswami, C., Shanahan, T.C., Schwartz, SA., 2002. Grape seed extract activates Thl cells in vitro. Clin. Diagn
  10. Nuttall, S. L., Kendall, M. J., Bombardelli, E., Morazzoni, P., 1998. An evaluation of the antioxidant activity of a standardized grape seed extract. J. Clin. Pharm. Ther. 23(5): 385-9 https://doi.org/10.1046/j.1365-2710.1998.00180.x
  11. Park, K. S., Kang, K. C., Kim, J. H., Adams, D. J., Johng, T.N., Paik, Y K, 1999. Differential inhibitory effects of protoberberines on sterol and chitin biosyntheses in Candida albicans. J. Antimicrob. Chemoth. 43: 667-674 https://doi.org/10.1093/jac/43.5.667
  12. Park, K S., Kang, K c., Kim, K Y, Jeong, P. Y, Kim, I H., Adams, D. J., Kim, J. H., Paik, Y K., 2001. HWY-289, a novel semi-synthetic protoberberine derivative with multiple target sites in Candida albicans. J. Antimicrob. Chemoth. 47: 513-519 https://doi.org/10.1093/jac/47.5.513
  13. Yamakoshi, J, Saito, M., Kataoka, S., Tokutake, S., 2002. Procyanidinrich-rich extract from grape seeds prevents cataract formation in hereditary cataractous (lCR/f) rats. J Agric Food Chem. 50(17): 4983-8. Lab. Immuol. 9(2): 470-476 https://doi.org/10.1021/jf0201632