Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Characterization of Squalene Synthase Inhibitor Isolated from Curcuma longa

울금(Curcuma longa)으로부터 분리한 squalene synthase 저해물질의 특성

  • Choi, Sung-Won (Research Institute of Biotechnology, Green Biotech Co. Ltd.) ;
  • Yang, Jae-Sung (Research Institute of Biotechnology, Green Biotech Co. Ltd.) ;
  • Lee, Han-Seung (Directed Evolution Team, GenoFocus Inc.) ;
  • Kim, Dong-Seob (Department of Food Science, Miryang National University) ;
  • Bai, Dong-Hoon (Department of Food Engineering, Dankook University) ;
  • Yu, Ju-Hyun (Korean Culture Center of Microorganisms)
  • Published : 2003.04.01
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Abstract

An inhibitor of squalene synthase, a key enzyme in the cholesterol biosynthetic pathways and a target for improved agents to lower plasma levels of low-density lipoprotein, was sequentially purified from Curcuma longa by acetone extraction, silica gel column chromatography, and sephadex LH-20 column chromatography. Active compound, YUF-01, was successfully purified and analyzed as $C_{20}H_{21}O_6$ by electron ionization mass spectrum. Through $^1H-NMR$ and $^{13}C-NMR$ analyses, YUF-01 was identified as curcumin, which showed strong inhibition of squalene synthase.

동맥경화증과 같은 심혈관 질환을 야기시키는 주요 위험요인인 혈중 콜레스테롤의 수준을 낮추기 위하여 콜레스테롤 생합성 과정의 속도조절단계 효소의 하나인 squalene synthase의 활성을 저해하는 물질을 분리 정제하여, 물질의 이화학적 특성과 생물학적 특성을 검토하였다. Squalene synthase 저해물질은 acetone extraction, ethyl acetate extraction, silica gel column chromatography, sephadex LH-20 column chromatography, 결정화 등을 이용하여 분리 정제하여 YUF-01을 얻었다. 기기분석을 통하여 구조분석을 행한 결과 YUF-01은 분자량 368, 분자식 $C_{20}H_{21}O_6$으로 분석되었으며 243과 421 nm 에서 UV-VIS 흡광을 나타내었고 $^{13}C$ NMR spectrum과 $^1H$ NMR spectrum을 검토하였을 때 aromatic ketone 구조인 curcuminoid 계통의 curcumin과 일치하였다. Squalene synthase에 대한 curcumin의 $IC_{50}$ 값은 $100{\mu}M$이었으며, non-competitive inhibitor로 작용하였다.

Keywords

References

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