Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Isolation of Listeria monocytogenes by Immunomagnetic Separation and Atomic Force Microscopy

  • Mercanolu, Birce (Hacettepe University, Faculty of Engineering, Food Engineering Department) ;
  • Aykut, S. (Hacettepe University, Faculty of Engineering, Food Engineering Department) ;
  • Ergun, M.Ali (Gazi University, Faculty of Medicine, Department of Medical Biology and Genetics) ;
  • Tan, Erdal (Ankara Nuclear Research and Training Center, Material Research Department)
  • Published : 2003.06.01
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Abstract

Listeria monocytogenes is a pathogen of major concern to the food industry and the potential cause of severe infections such as listeriosis. Early detection of this foodborne pathogen is important in order to eliminate its potential hazards. So, immunomagnetic separation (IMS) has been suggested as a means of reducing the total analysis time and for improving the sensitivity of detection. Atomic force microscopy (AFM) has been used for measuring the topographic properties of sample surfaces at nanometer scale. In this study, we used AFM to confirm both the sensitivity and the specificity of IMS. Regarding AFM analysis, the length and the width of the bacteria, which were in agreement with literature values, were found to be 2.993 $\mu\textrm{m}$ and 0.837 $\mu\textrm{m}$, respectively. As a result, AFM helped us both characterize and measure the bacterial and bead structures.

Keywords

References

  1. Int. J. Food Microbiol. v.23 Presence, detection and growth of Listeria monocytogenes in seafoods: a review Ben Embarek,P.K. https://doi.org/10.1016/0168-1605(94)90219-4
  2. Physical Rev. Lett. v.56 Atomic force microscopy Binnig,G.;H.Rohrer;C.Gerber https://doi.org/10.1103/PhysRevLett.56.930
  3. J. Dairy Res. v.55 Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products Dominguez,L.;J.F.Fernandez;V.Briones;J.L.Blanco;G.Suarez https://doi.org/10.1017/S0022029900033367
  4. Dynal Bioscience Product Catalogue
  5. Scanning v.21 Numerical chromosome abnormalities detected by atomic force microscopy Ergun,M.A.;E.Tan;F.I.Sahin;A.Menevse https://doi.org/10.1002/sca.4950210302
  6. Encyclopedia of Food Microbiology v.2 Detection by commercial particle-based assays Fratamico,P.M.;C.G.Crawford;R.K.Robinson(ed.)
  7. Rad. Phys. Chem. v.50 An atomic force microscopic study of the surfaces of polyethylene and polycarbonate films irradiated with gamma rays Guven,O.;A.Alacakir;E.Tan https://doi.org/10.1016/S0969-806X(97)00002-9
  8. J. Food Prot. v.64 Combination of immunomagnetic separation and polymerase chain reaction for the simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples Hsih,H.Y.;H.Y.Tsen
  9. Microorganisms in Foods 5: Microbiological Specifications of Food Pathogens International Commission on Microbiological Specifications for Foods (ICMSF)
  10. J. Microbiol. Meth. v.31 Immunomagnetic separation of Listeria Monocytogenes for flow cytometric determination of viable cells in liquid Jacobsen,C.N.;C.Fremming;M.Jakobsen https://doi.org/10.1016/S0167-7012(97)00084-5
  11. Archiv fur Lebensmittelhygiene v.53 Immunomagnetic separation and a cultural reference method for detection of Salmonella spp. in foods Mercanoglu,B.;S.A.Aytac
  12. Fleischwirtschaft v.76 Coupling of immunomagnetic separation and ELISA for the rapid detection of Salmonella in foods Molla,B.;J.Kleer;H.J.Sinell
  13. Rapid Analysis Techniques in Food Microbiology Microbiological applications of immunomagnetic techniques Patel,P.D.;P.D.Patel(ed.)
  14. A. Review. J. Appl. Bacteriol. v.78 The application of magnetic separations in applied microbiology. Safarik,I.;M.Safarikova;S.J.Forsythe https://doi.org/10.1111/j.1365-2672.1995.tb03102.x
  15. Microbiol. Immunol. v.42 Surface characteristics of Gram-negative and Gram-positive bacteria in an atomic force microscope image Umeda,A.;M.Saito;K.Amako