Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Different Kind of Plant Oil Sources on Serum and Hepatic Lipid levels of Streptozotocin-induced Diabetic Mice

Streptozotocin으로 유발한 당뇨 마우스에서 식물성 지방 급원에 따른 혈액 및 간조직의 지질 조성

  • Lee, Sung-Hyeon (Agriproduct Science Division, National Rural Living Science Institute, NIAST, RDA) ;
  • Chun, Hye-Kyung (Agriproduct Science Division, National Rural Living Science Institute, NIAST, RDA) ;
  • Park, Hong-Ju (Agriproduct Science Division, National Rural Living Science Institute, NIAST, RDA) ;
  • Lee, Yeon-Sook (Dept. of Food and Nutrition, Seoul National University) ;
  • Chang, Soon-Ok (Dept. of Food and Nutrition, The University of Suwon)
  • 이성현 (농촌진흥청 농업과학기술원 농촌생활연구소 농산물가공이용과) ;
  • 전혜경 (농촌진흥청 농업과학기술원 농촌생활연구소 농산물가공이용과) ;
  • 박홍주 (농촌진흥청 농업과학기술원 농촌생활연구소 농산물가공이용과) ;
  • 이연숙 (서울대학교 식품영양학과) ;
  • 장순옥 (수원대학교 식품영양학과)
  • Published : 2003.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to compare the effects of different plant oil sources on lipid metabolism of insulin dependent diabetic mice. Streptozotocin-induced (65 mg/kg B.W.) diabetic mice were fed four kinds of experimental diets with 20% lipid, which composed of 10% lard, and 10% soy bean oil (SBO), 10% rice germ oil (RGO), 10% rice brab oil (RBO) and 10% sesame oil (SSO) respectively, for 7 weeks. Diet intake, body weight, organs weights and lipids levels of serum, liver and feces were measured. There was no significant difference in diet intake and body weight among experimental groups. But the concentrations of serum triglyceride of RGO and RBO groups, and of serum total cholesterol of RGO and SSO groups were lower than those of the others. The hepatic total cholesterol level was the lowest in RGO group. The contents of total lipid and total cholesterol excreted in feces of RGO and RBO groups were higher than those of SBO and SSO groups. These results suggested that rice germ oil can effectively reduce serum triglyceride level and hepatic total cholesterol concentration of insulin dependent diabetic mice than rice bran oil, soy bean oil and sesame oil, and hypolipidemic effect of rice germ oil be due to increasing fecal lipid excretion. But we need to investigate the more detailed factors on lipid reducing effect in rice germ oil and rice bran oil.

본 연구는 식물성 지방급원이 당뇨모델의 지질대사에 미치는 영향을 검토하고자 수행하였다. 실험 모델은 STZ의 투여로 유발된 인슐린의존형 당뇨 마우스를 이용하였고, 4종류(라아드 10%에 각각 대두유, 쌀눈기름, 쌀겨 기름, 참기름 10% 첨가)의 실험식이를 7주간 급여하여 체내 지질 함량 및 대변으로의 배설량을 조사하였다. 그 결과 실험동물의 식이섭취량과 체중 및 장기 무게는 실험군 사이에 큰 차이가 없었으나, 혈청 중성지방은 쌀눈기름 및 쌀겨기름 첨가군에서 다른 실험군들보다 낮은 경향을 보였고, 간의 총콜레스테롤 함량은 쌀눈기름 첨가군에서 대조군보다 낮은 경향을 보였으며, 간의 총지질 함량도 쌀눈기름 첨가군에서 대조군보다 낮은 경향을 보였다. 그리고 대변으로의 총지질 및 총콜레스테롤 배설량은 쌀눈기름 첨가군과 쌀겨기름 첨가군에서 대조군인 대두유군보다 유의하게 많은 것으로 나타났다. 이상의 결과로부터 쌀눈기름과 쌀겨기름 급여군에서 체내 지질 함량이 낮았고 쌀눈기름군에서 쌀겨기름군보다 낮은 경향을 보였다. 그러므로 쌀눈기름은 인슐린의존형 당뇨마우스에서 체내 지질 함량 저하 효과가 있는 것으로 밝혀졌으며, 이는 쌀눈기름의 대변중 총지질 및 총콜레스테롤 배설량 증가와 관련된 것으로 평가되었다. 따라서 쌀눈기름은 대두유나 참기름보다 당뇨병 모델의 체내 지질대사에 개선 효과가 있는 것으로 보인다.

Keywords

References

  1. Dong SR, Oh DH, Kim SW, Yang IM, Kim JW, Kim YS, Choi YG. 1988. Relationship among complications serum lipids and lipoprotein profiles in diabetics. Kor J Internal Medicine 35: 510-519
  2. Ministry and health welfare. 1999. '98 National health and nutrition survey report. Korea institute for health and social affairs
  3. Kim WJ, Min HG, Choi YK, Lee TH, Huh KB, Shin SH. 1998. Diabetes. Kor Diabetes Association
  4. Cho NH. 2000. Analysis of epidemiological characteristic and dangerous factor. Kor J Lipid and Arteriosclerosis 10: 11-16
  5. Huh KB. 1992. Insulin resistance and chronic degenerative diseases. J Kor Diabetes 16: 93-98
  6. Chung YS, Park SW, Kim JA, Lee EJ, Lim SK, Kim KR, Lee HC, Huh KB, Paik IK, Yoon JY, Kim HS, Chang HJ, Lee JH. 1993. Effects of $\omega3$ fatty acid supplementation on serum lipids in patients with non-insulin dependent diabetes mellitus. J Kor Diabetes 17: 267-274
  7. Thomsen C, Rasmussen O, Lousen T, Holst JJ, Fenselau S, Schrezenmeir J, Hermansen K. 1999. Differential effects of saturated and monounsaturated fatty acids on postprandial lipemia and incretin response in healthy subjects. Am J Clin Nutr 69: 1135-1143
  8. Sharma RD, Rukmini C. 1986. Rice bran oil and hypocholesterolemia in rat. Lipids 21: 715-717 https://doi.org/10.1007/BF02537246
  9. Seetharamaiah GS, Chandrasekhara N. 1989. Studies on hypocholesterolemic activity of rice bran oil. Atherosclerosis 78: 219-223 https://doi.org/10.1016/0021-9150(89)90226-8
  10. Sunitha T, Manorama R, Rukmini C. 1997. Lipid profile of rats fed blends of rice bran oil in combination with sunflower and safflower oil. Plant Food Hum Nutr 51: 219-230 https://doi.org/10.1023/A:1007993016796
  11. Koba K, Liu JW, Bobik E, Sugano M, Huang YS. 2000. Cholesterol supplementation attenuate the hypocholesterolemic effect of rice bran oil. J Nutr Sci Vitaminol 46: 58-64 https://doi.org/10.3177/jnsv.46.58
  12. Nakamura H. 1996. Effect of $\gamma$ -oryzanol on hepatic cholesterol biosynthesis and fecal excretion of cholesterol metabolites. Radioisotopes 25: 371-374
  13. Rong N, Ausman LM, Nicolosi RJ, 1997. Oryzanol decrease cholesterol absorption and aortic fatty streaks. Lipids 32: 303-309 https://doi.org/10.1007/s11745-997-0037-9
  14. Bhathena SJ, Berlin E, Judd JT, Kim YC, Law JS, Bhagavan HN, Ballard-Barbash R. 1991. Effects of omega 3 fatty acids and vitamin E on hormones involved in carbohydrate and lipid metabolism in men. Am J Clin Nutr 54: 684-688
  15. Raghuram TC, Tao UB, Rukmini C. 1989. Studies on hypolipidernic effects of dietary rice bran oil in human subjects. Nutr Res Int 39: 889-895
  16. Park YJ. 1999. Effects of vitamin E supplementation and fat source on kidney function and lipid peroxidation in adriamycin treated rats. PhD Thesis. Seoul National University
  17. Robert L, Dobbins L, Szczepaniak S, Brandon B, Victoria E, Jeffrey M, Denis MJ. 2001. Prolonged inhibition of muscle carnitine palmitoyltrans ferase-1 promotes intramyocellular lipid accumulation and insulin resistance in rats. Diabetes 50: 123-130 https://doi.org/10.2337/diabetes.50.1.123
  18. Folch J, Less M, Sloanestanley GH. 1957. A simple method for the isolation and purification of total lipid from animal tissues. J Bio Chem 226: 497-509
  19. Zlatkis A, Zak B. 1969. Study of a new cholesterol reagent. Anal Biochem 29: 143-148 https://doi.org/10.1016/0003-2697(69)90017-7
  20. Biggs HG, Erikson TA, Moorehead WR. 1975. A manual colorimetric assay of triglyceride in serum. Clinical Chem 21: 437-441
  21. Forman S, Estilow ML, Vailenko P. 1986. Streptozotocin diabetes alters immunoreactive $\beta$-endorphin levels and pain perception after 8wk in female rats. Diabetes 35: 1309-1313 https://doi.org/10.2337/diabetes.35.12.1309
  22. Wilson TA, Ausman LM, Lawton CW, Hegsted DM, Nicolosi RJ. 2000. Comparative cholesterol lowering properties of vegetable oils: beyond fatty acids. J Am Coll Nutr 19: 601-607 https://doi.org/10.1080/07315724.2000.10718957
  23. Edwards MS, Radcliffe JD. 1994. A comparison of the effect of rice bran oil and corn oil on lipid status in the rat. Biochemical Archives 10: 87-94
  24. Lee SH, Chun HK, Lee YS. 1999. The study for nutritional composition of rice germ oil. The Annual Research Report of National Rural Living Science. p 351-396
  25. Purushothama S, Ranina PL, Hariharan K. 1995. Effect of long term feeding of rice bran oil upon lipids and lipoproteins in rat. Mol Cell Biochem 146: 63-69 https://doi.org/10.1007/BF00926883
  26. Lee SH, Chun HK, Lee YS. 2001. The effects of rice germ oil supplement on blood glucose, serum lipid and blood pressure levels in diabetic patients. Kor J Lipid and Arteriosclerosis 11: 548-557

Cited by

  1. Development of a Beverage Using the Extracts from Bombyx mori L., Morus alba L., Dioscoreae rhizome and Inonotus obliquus vol.18, pp.6, 2011, https://doi.org/10.11002/kjfp.2011.18.6.844