Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Antimicrobial Activity of Methanol Extract from Rheum tanguticum against Food Hazardous Microorganisms and the Composition of the Extract

식품위해성 미생물에 대한 대황(Rheum tanguticum) 메탄올 추출물의 항균활성 및 성분분석

  • 임미경 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과)
  • Published : 2003.08.01
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Abstract

Daehwang (Rheum tanguticum) was extracted by methanol, and the antimicrobial activities of the extract, against some food hazardous microorganisms, were investigated by the paper disc method. The minimum inhibitory concentration (MIC) of the extract against the microorganisms was determined, and the extract components were analyzed by GC/MS. The Daehwang extract showed the strongest antimicrobial effect against P. aeruginosa. The MICs on S. aureus, P. aeruginosa and S. typhimurium were 250, 300 and 300mg/$m\ell$, respectively. In the methanol extracts from Daehwang, 120 different compounds were separated, with the extraction yield of the phenolic compounds being relatively high (29.85%). Substances with antimicrobial activity, such as 2-methoxy-phenol and 4-vinyl-2-methoxy-phenol were detected in the study.

식품 위해성 세균과 곰팡이에 대하여 대황 메탄을 추출물의 항균활성을 paper disc법으로 조사하였고 최소생육저해농도를 결정하였다. 또한 GC/MS를 이용하여 대황 메탄을 추출물에서 분리된 각 peak의 성분과 함량을 분석하였다. 대황 메탄을 추출물은 p. aeruginosa에 대해 강한 항균활성을 보였으며 S. aureus와 S. typhimurium에 대해서도 상당한 항균활성을 나타내었다. 대황 메탄을 추출물은 250mg/$m\ell$농도에서 S. aureus의 생육을 저지시켰으며 300mg/$m\ell$농도에서 p. aeruginosa와 S. typhimurium의 생육을 억제시켰다. 대황 메탄을 추출물을 GC/MS로 분석한 결과 전체 120가지의 성분이 분석되었으며 이 중 추출물에 함유된 ketones 화합물이 39%로 가장 많은 부분을 차지하고 있었다. 항균물질로 알려진 phenol성 화합물인 2-methoxy-phenol, 4-vinyl-2-methoxy-phenol 등이 대황 메탄을 추출물에서도 검출되었다.

Keywords

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