Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

The Optimal Producing Conditions of Bacteriocin Produced by Lactobacillus sp. FF-3 Isolated from Korean Dongchimi

동치미에서 분리한 Lactobacillus sp. FF-3가 생산하는 bacteriocin의 최적 생산조건

  • 박진철 (동아대학교 응용생명공학부 생명공학과) ;
  • 차재영 (동아대학교 응용생명공학부 생명공학과) ;
  • 권오창 (동아대학교 응용생명공학부 생명공학과) ;
  • 조영수 (동아대학교 응용생명공학부 생명공학과)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The optimal culture conditions on bacteriocin producing of Lactobacillus sp. FF-3 isolated from Korean Dongchimi, were studied for enhancing its production with regard to environmental and nutritional factors. The optimal cultivation time, initial pH and temperature were 21 hours, pH 7.0 and 30∼37$^{\circ}C$ respectively. Optimal compositions of culture medium for bacteriocin production were glucose 3% as carbon source, tryptone 4% as nitrogen source, and manganese sulfate 0.005% as inorganic salt with other basal components. The maximum antimicrobial activity was 484 BU/mL under the optimal culture condition.

동치미로부터 분리된 Latobacillus sp. FF-3의 Bacteriocin 생산에 대한 최적 배양조건과 배양 특성을 조사하였다. Bacteriocin 생산을 위한 최적 생산조건은 MRS(proteose peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, polysorbate 80 0.1%, ammonium citrate 0.2%, sodium acetate 0.5%, magnesium sulfate 0.01%, manganese sulfate 0.005%, dipotassium phosphate 0.2%) 배지를 기본으로 하여 21시간 배양한 결과, 배양온도는 30∼37$^{\circ}C$, 초기 pH는 7.0 으로 확인되었다. 최적 생산배지조성으로 탄소원은 glucose 3%, 질소원은 tryptone 4%, 무기염류는 manganese sulfate 0.005%가 bacteriocin 생성에 최적 조성으로 나타났으며, 최적 배지상에서 항균활성은 최대 484 BUf/mL로 나타났다.

Keywords

References

  1. Dahiya, R.S. and Speck, M.L. (1968) Hydrogen peroxide formation by Lactobacilli and its effect on Staphylococcus aureus. J. Dairy Sci., 51, 1568-1572 https://doi.org/10.3168/jds.S0022-0302(68)87232-7
  2. Reeves, P. (1965) The Bacteriocins. Bacteriol. Rev., 29, 24-45
  3. Lee, J.H. and Chang, H.I. (1994) Characteristics of the bacteriocin produced from Streptococcus salivarius subsp. thermophilus. Kor. J. Appl. Microbiol. Biotechnol., 22, 7-12
  4. Yoo, J.Y., Lee, I.S.,Chung, K.S. and Nam, Y.J. (1991) Isolation and properties of bacteriocin-producing microorganisms. Kor. J. Appl. Microbiol. Biotechnol., 19, 8-13
  5. Barefoot, S.F. and Klaenhammer, T.R. (1984) Purification and characterization of the Lactobacillus acidophilus bacteriocin, lactacin B. Antimicrobial Agents and Chemotheraphy, 26, 328-334 https://doi.org/10.1128/AAC.26.3.328
  6. Rammelsberg, M., Muller, E. and Radler, F. (1990) Caseicin 80: purification and characterization fo a new bacteriocin from Lactobacillus casei. Arch. Microbiol., 154, 249-252 https://doi.org/10.1007/BF00248963
  7. Mortvedt, C.I., Nissen-Meyer, J., Sletten, K. and Nes, I. F. (1991) Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake LA5. Appl. Environ. Microbiol., 57, 1829-1834
  8. Hirsch, A. (1951) Growth and nisin production of a strain of Streptococcus lactis. J. Gen. Microbiol., 5, 208-221 https://doi.org/10.1099/00221287-5-1-208
  9. Kim, S.K., Lee, S.J., Baek, Y.J. and Park Y.H. (1994) Isolation of bacteriocin-producing Latococcus sp. HY 449 and its antimicrobial characteristics. Kor. J. Appl. Microbiol. Biotechnol., 22, 259-265
  10. 박연희, 권정주, 조도현 (1983) 한국농화학회지, 26, 35-39
  11. 정영건, 안장연, 권오진, 강주희 (1989) Lactobacillus acidophilus Bacteriocin 의 성상에 관한 연구. 한국산업미생물학회지, 17, 94-98
  12. 유진영, 최신양, 진영옥, 구영조, 정건섭 (1989) 회분식 및 연속식 배양시 Nisin 의 생산 특성. 한국산업미생물학회지, 17, 504-508
  13. Hertman, I. and Ben-Gurion, R. (1958) A study of pesticin biosynthesis. J. Gen. Microbiol., 21, 135-143
  14. Shahani, K.M., Vakil, J.R. and Kilara, A. (1977) Natural antibiotic activity of Lactobacillus acidophilus and bulgaricus. II. Isolation of acidophilin from Lactobacillus acidophilus. Cultured Dairy Productis J., 12, 8-11
  15. Femandes, C.F., Shahani, K.M. and Amer, M.A. (1988) Effect of nutrient media and bile salts on growth and antimicrobial activity of Lactobacillus acidophilus. J. Dairy Sci., 71, 3222-3229 https://doi.org/10.3168/jds.S0022-0302(88)79927-0
  16. Reddy, G.V., Shahani, K.M., Friend, B.A. and Chandra, R. C. (1983) Natural antibiotic activity of Lactobacillus acidophilus and bulgaricus. III. Production and partial purification of bulgarican from Lactobacillus bulgaricus. Cultured Dairy Products J., 18, 15-19
  17. Hirsch, A. (1951) Various antibiotics from one strain of Streptococcus lactis. Nature, 167, 1031-1032 https://doi.org/10.1038/1671031a0
  18. Chung, W.B., Soe, W.S., Cha, J.Y. and Cho, Y.S. (2003) Isolation and characterization of Lactobacillus sp. FF-3 for probiotics production from Korean Dongchimi. Korean Journal of Food Preservation, 10, 406-410
  19. Toba, T., Samant, S.K. and Itoh, T. (1991) Assay system for detecting bacteriocin in microdilution wells. Letters in Appl. Microbiol., 13, 102-104 https://doi.org/10.1111/j.1472-765X.1991.tb00581.x
  20. Rayman, K. and Hurst, A. (1984) Nisin: Properties, biosynthesis and fermentation, Vandamme, E.J. (Editor), Biotechnology of Industrial Antibiotics, Marcel Dekker Inc., p. 607-628
  21. Grushina, V.A. Baranova, I.P. and Egorov, N.S. (1979) Nisin accumulation dynamic in a Streptococcus lactis culture. Appl. Biochem. Microbiol., 9, 44-47
  22. Baranova, I.P. and Egorov, N.S. (1969) Effect of composition of medium and cultivation conditions on growth of Streptococcus lactis and nisin biosynthesis. Appl. Biochem. Microbiol., 5, 137-143
  23. Kozlova, Y.I., Egorov, N.S., Baranova, I.P. nad Maksimov, Y.N. (1972) Metabolic kinetics of Streptococcus lactis on initial and optimal media. Microbiology, 41, 893-897
  24. Yoo, J.Y., Lee, I.S., Chung, K.S., Choi, S.Y., Koo, Y.J. and Kwon, D.J. (1992) Cultural conditions of Lactococcus sp. 1112-1 for production of bacteriocin-like substance. Kor. J. Appl. Microbiol. Biotechnol., 20, 183-189
  25. Kozlova, Y.I., Golikova, T.I., Baranova, I.P. and Egorov, N.S. (1979) Investigation on the influence of $KH_2PO_4$ on the growth of Streptococcus lactis and nisin synthesis at constant pH values of the medium. Mikrobiologiya, 48, 443-446