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The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Anti-Inflammatory Activity of Elsholtzia splendens

  • Published : 2003.03.01
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Abstract

Elsholtzia splendens Nakai has been used in North-East Asia as an ingredient of folk medicines for treating cough, headache and inflammation. The present investigation was carried out to establish its in vivo anti-inflammatory activity using several animal models of inflammation and pain. The 75% ethanol extract of the aerial part of E. splendens significantly inhibited mouse croton oil-induced, as well as arachidonic acid-induced, ear edema by oral administration (44.6% inhibition of croton oil-induced edema at 400 mg/kg). This plant material also showed significant inhibitory activity against the mouse ear edema induced by multiple treatment of phorbol ester for 3 days, which is an animal model of subchronic inflammation. In addition, E. splendens exhibited significant analgesic activity against mouse acetic acid-induced writhing (50% inhibition at 400 mg/kg), while indomethacin (5 mg/kg) demonstrated 95% inhibition. E. splendens ($5-100{\;}{\mu}g/mL$) significantly inhibited $PGE_2$ production by pre-induced cyclooxygenase-2 of lipopolysaccharide-treated RAW 264.7 cells, suggesting that cyclooxygenase-2 inhibition might be one of the cellular mechanisms of anti-inflammation.

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References

  1. Bae, K. (Ed.), in The medicinal plants of Korea. Kyohaksa Co., Seoul, pp.435, (2000)
  2. Bentley, G. A., Newton, S. H., and Star, J., Studies on the anti-nociceptive action of alpha-agonist drugs and their interactions with opioid mechanisms. Brit. J. Pharmacol., 79, 125-134 (1983) https://doi.org/10.1111/j.1476-5381.1983.tb10504.x
  3. Chi, Y. S., Cheon, B. S., and Kim, H. P., Effect of wogonin, a plant flavone from Scutellaria radix, on the suppression of cyclooxygenase-2 and the induction of inducible nitric oxide synthase in lipopolysaccharide-treated RAW 264.7 cells. Biochem. Pharmacol., 61, 1195-1203 (2001) https://doi.org/10.1016/S0006-2952(01)00597-4
  4. Goppelt-Struebe, M., Molecular mechanisms involved in the regulation of prostaglandin biosynthesis by glucocorticoids. Biochem. Pharmacol., 53, 1389-1395 (1997) https://doi.org/10.1016/S0006-2952(97)00018-X
  5. Isobe, T., and Noda, Y., Studies on chemical constituents of Elsholtzia herb. J. Chem. Soc. (Japan), 4, 423-425 (1992)
  6. Kim, H. K., Namgoong, S. Y., and Kim, H. P., Anti-inflammatory activity of flavonoids: Mice ear edema inhibition. Arch. Pharm. Res., 16, 18-24 (1993) https://doi.org/10.1007/BF02974122
  7. Kim, H. K., Son, K. H., Chang, H. W., Kang, S. S., and Kim, H.P., Inhibition of rat adjuvant-induced arthritis by gingketin, a biflavone from Gingko biloba leaves. Planta Med., 65, 465-467 (1999) https://doi.org/10.1055/s-2006-960815
  8. Kobold, U., Vostrowsky, O., Bsetman, H. J., Bisht, J. C., Pant, A. K., Melkani, A. B., and Mathela, C. S., Terpenoids from Elsholtzia species, 2. Constituents of essential oils from a new chemotype of Elsholtzia cristata. Planta Med., 55, 268-271 (1987)
  9. Lee, W. C. (Ed.), in Standard illustration of Korean plants. Academy, Seoul, pp.302-303 (1996)
  10. Lee, Y., Lee, I., Woo, W., and Park, C., Flavonoids of Elsholtzia cristata. Arch. Pharm. Res., 11, 247-249 (1988) https://doi.org/10.1007/BF02861317
  11. Mossman, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. Methods, 65, 55-63 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  12. McMurray, R. W., and Hardy, K. J., COX-2 inhibitors: today and tomorrow. Am. J. Med. Sci., 323, 181-189 (2002) https://doi.org/10.1097/00000441-200204000-00003
  13. Stanley, P. L., Steiner, S., Havens, M., and Tramposch, K. M., Mouse skin inflammation induced by multiple topical applications of 12-O-tetradecanoylphorbol-13-acetate. Skin Pharmacol., 4, 262-271 (1991) https://doi.org/10.1159/000210960