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The Pharmaceutical Society of Korea (대한약학회)
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The Role of Ascorbic Acid on the Redox Status and the Concentration of Malondialdehyde in Streptozotocin-Induced Diabetic Rats

  • Choi, Hee-Jung (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Je, Hyun-Dong (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Jeong, Ji-Hoon (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Min, Young-Sil (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Choi, Tae-Sik (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Park, Joon-Hong (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Shin, Chang-Yell (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung Ang University)
  • Published : 2003.03.01
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Abstract

We investigated the role of ascorbic acid on the redox status in streptozotocin-induced diabetic rats. In the plasma of diabetic rats, the ratio of reduced/total ascorbic acid was significantly decreased as compared with normal control. Ascorbic acid supplementation increased the reduced and total ascorbic acid contents as compared with diabetic control. In the rutintreatment group, reduced and total contents of ascorbic acid were significantly decreased, however, the ratio of reduced/total contents of ascorbic acid had no difference as compared with diabetic rats. In the insulin-treatment group, this ratio is not significantly different as compared with diabetic control. However, in the insulin plus ascorbic acid treatment group, reduced form and the ratio of reduced/total ascorbic acid were significantly increased as compared with diabetic control. In addition, we measured the contents of malondialdehyde (MDA) in the plasma of diabetic rats. The contents of MDA was increased as compared with normal control, however, in insulin-treatment group, the contents of MDA was decreased as compared with diabetic rats. Ascorbic acid had no effects on the increases of MDA in diabetic rats. In conclusion, plasma ascorbic acid level and its reduced/total ratio reflects the status of the oxidative stress in the diabetic rats. Supplement of ascorbic acid did not correct the ratio of the reduced/total ascorbic acid. However, supplement of insulin and ascorbic acid corrected the ratio of reduced/total ascorbic acid.

Keywords

References

  1. Abou-Seif, M. A., Rabia, A and Nasr, M., Antioxidant status, erythrocyte membrane lipid peroxidation and osmotic fragility in malignant lymphoma patients. Clin. Chem. Lab. Med., 38, 737-742 (2000) https://doi.org/10.1515/CCLM.2000.104
  2. Abraham, E. C., Perry, R. E. and Stallings, M., Application of affinity chromatography for separation and quantitation of glycosylated hemoglobins. J. Lab. Clin. Med., 102, 187-197 (1983)
  3. Afanas'ev, I. B., Dorozhko, A. I., Brodskii, A. V.. Kostyuk, V. A. and Potapovitch, A. I., Chelating and free radical scavenging H. J. Choi et al. mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation. Biochem. Pharmacol., 38, 1763-1769 (1989) https://doi.org/10.1016/0006-2952(89)90410-3
  4. Afanas'ev, I. B., Ostrachovich, E. A. and Korkina, L. G., Effect of rutin and its copper complex on superoxide formation and lipid peroxidation in rat liver microsomes. FEBS Lett., 425, 256-258 (1998) https://doi.org/10.1016/S0014-5793(98)00244-0
  5. Baker, E. N., Blundell, T. L., Cutfield, J. F., Cutfield, S. M., Dodson, E. J., Dodson, G. G., Hodgkin, D. M., Hubbard, R. E., Isaacs, N. W. and Reynolds, C. D., The structure of 2Zn pig insulin crystals at 1.5 A resolution. Philos. Trans. R. Soc. Land. B. Biol. Sci., 319, 369-456 (1988) https://doi.org/10.1098/rstb.1988.0058
  6. Berger, T. M., Polidori, M. C., Dabbagh, A., Evans, P. J., Halliwell, B., Morrow, J. D., Roberts, L. J. and Frei, B., Antioxidant activity of vitamin C in iron-overloaded human plasma. J. Biol. Chem., 272, 15656-15660 (1997) https://doi.org/10.1074/jbc.272.25.15656
  7. Bors, W., Heller, W., Michel, C. and Saran, M., Flavonoids as antioxidants: determination of radical-scavenging efficiencies. Methods Enzymol., 186, 343-355 (1990) https://doi.org/10.1016/0076-6879(90)86128-I
  8. Cay, M., Naziroglu, M., Simsek, H., Aydilek, N., Aksakal, M. and Demirci, M., Effects of intraperitoneally administered vitamin C on antioxidative defense mechanism in rats with diabetes induced by streptozotocin. Res. Exp. Med. (Berl), 200, 205-213 (2001)
  9. Chevenne, D., Trivin, F. and Porquet, D., Insulin assays and reference values. Diabetes Metab., 25, 459-476 (1999)
  10. Eriksson, J. and Kohvakka, A., Magnesium and ascorbic acid supplementation in diabetes mellitus. Ann. Nutr. Metab., 39, 217-223 (1995) https://doi.org/10.1159/000177865
  11. Fukunaga, K., Takama, K. and Suzuki, T., High-performance liquid chromatographic determination of plasma malondialdehyde level without a solvent extraction procedure. Anal. Biochem., 230, 20-23 (1995) https://doi.org/10.1006/abio.1995.1431
  12. Gibbons, E., Allwood, M. C., Neal, T. and Hardy, G., Degradation of dehydroascorbic acid in parenteral nutrition mixtures. J. Pharm. Biomed. Anal., 25, 605-611 (2001) https://doi.org/10.1016/S0731-7085(00)00589-6
  13. Hultqvist, M., Hegbrant, J., Nilsson-Thorell, C., Lindholm, T., Nilsson, P., Linden, T. and Hultqvist-Bengtsson, U., Plasma concentration of vitamin C, vitamin E and/or malondialdehyde as markers of oxygen free radical production during hemo-dialysis. Clin. Nephrol., 47,37-46 (1997)
  14. Iwata, T., Yamaguchi, M., Hara, S., Nakamura, M. and Ohkura, Y, Determination of total ascorbic acid in human serum by high-performance liquid chromatography with fluorescence detection. J. Chromatogr., 344, 351-355 (1985) https://doi.org/10.1016/S0378-4347(00)82039-3
  15. Khan, M. M. and Martell, A. E., Metal ion and metal chelate catalyzed oxidation of ascorbic acid by molecular oxygen. I. Cupric and ferric ion catalyzed oxidation. J. Am. Chem. Soc., 89, 4176-4185 (1967) https://doi.org/10.1021/ja00992a036
  16. Li, M., Suzuki, E. and urata, T., Effects of 2,3-diketo-L-gulonic acid on the oxidation of yolk lipoprotein. Biosci. Biotechnol. BioChem., 65, 599-604 (2001) https://doi.org/10.1271/bbb.65.599
  17. Lykkesfeldt, J., Determination of ascorbic acid and dehydro-ascorbic acid in biological samples by high-performance liquid chromatography using subtraction methods: reliable recucion with tris[2-carboxyethyl]phosphine hydrochloride. Anal. Biochem., 282, 89-93 (2000) https://doi.org/10.1006/abio.2000.4592
  18. Lykkesfeldt, J., Loft, S. and Poulsen, H. E., Determination of ascorolc acid and dehydroascorbic acid in plasma by high-pe formance liquid chromatography with coulometric detection are they reliable biomarkers of oxidative stress? Anal. Biochem., 229, 329-335 (1995) https://doi.org/10.1006/abio.1995.1421
  19. McLennan, S., Yue, D. K., Fisher, E., Capogreco, C., Heffernan, S. Ross, G. R and Turtle, J. R, Deficiency of ascorbic acid in exoerimental diabetes. Relationship with collagen and pc yo pathway abnormalities. Diabetes, 37, 359-361 (1988) https://doi.org/10.2337/diabetes.37.3.359
  20. Nielsen, F., Mikkelsen, B.B., Nielsen, J.B., Andersen, H.R and Grandjean, P, Plasma malondialdehyde as biomarker for ox da:ive stress: reference interval and effects of life-style factors. Clin. Chem., 43, 1209-1214 (1997)
  21. Olsen H. B., Ludvigsen, S. and Kaarsholm, N. C., Solution structure of an engineered insulin monomer at neutral pH. Biochemistry, 35, 8836-8845 (1996) https://doi.org/10.1021/bi960292+
  22. Omura, H., Tomita, Y., Fujiki, H., Shinohara, K. and Murakami, H., Breaking action of reductones related to ascorbic acid on nucleic acids. J. Nutr. Sci. Vitaminol., 24, 263-270 (1978) https://doi.org/10.3177/jnsv.24.263
  23. Palmer, A. M., Thomas, C. R, Gopaul, N., Dhir, S., Anggard, E. E. Poston, L. and Tribe, R M., Dietary antioxidant supple-mentation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocin-diabetic rat. Diabetologia, 41, 148-156 (1998) https://doi.org/10.1007/s001250050883
  24. Pence, L. A. and Mennear, J. H., Inhibition effect of dehydroascorbic acid on insulin secretion from mouse pancreatic islets. Toxicol. Appl. Pharmacol., 50, 57-65 (1979) https://doi.org/10.1016/0041-008X(79)90492-7
  25. Pulico, R, Bravo, L. and Saura-Calixto, F, Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J. Agric. Food. Chem., 48, 3396-3402 (2000) https://doi.org/10.1021/jf9913458
  26. Reynolds, T. M., Chemistry of nonenzymic browning. II. Adv. Food Res., 14, 167-283 (1965) https://doi.org/10.1016/S0065-2628(08)60149-4
  27. Robak, J. and Gryglewski, R J., Flavonoids are scavengers of superoxide anions. Biochem. Pharmacol., 37, 837-841 (1988) https://doi.org/10.1016/0006-2952(88)90169-4
  28. Seghieri, G., Martinoli, L., di Felice, M., Anichini, R, Fazzini, A., Cluti, M., Miceli, M., Gaspa, L. and Franconi, F, Plasma and platelet ascorbate pools and lipid peroxidation in insulindependent diabetes mellitus. Eur. J. Clin. Invest., 28, 659-663 (1998) https://doi.org/10.1046/j.1365-2362.1998.00339.x
  29. Simpson, G. L. and Ortwerth, B. J., The non-oxidative degradation of ascorbic acid at physiological conditions. Biochim. Biophys. Acta, 1501, 12-24 (2000) https://doi.org/10.1016/S0925-4439(00)00009-0
  30. Singal, P K., Bello-Klein, A., Farahmand, F. and Sandhawalia, V., Oxidative stress and functional deficit in diabetic cardiomyopathy. Adv. Exp. Med. Biol., 498, 213-220 (2001) https://doi.org/10.1007/978-1-4615-1321-6_27
  31. Speek, A. J., Schrijver, J. and Schreurs, W. H., Fluorometric determination of total vitamin C in whole blood by high- performance liquid chromatography with pre-column derivatization. J. Chromatogr., 305, 53-60 (1984) https://doi.org/10.1016/S0378-4347(00)83313-7
  32. Therasse, J. and Lemonnier, F., Determination of plasma lipo-peroxides by high performance liquid chromatography. J. Chromatogr., 413, 237-241 (1987) https://doi.org/10.1016/0378-4347(87)80232-3
  33. Travacio, M., Maria Polo, J. and Llesuy, S., Chromium(VI) induces oxidative stress in the mouse brain. Toxicology, 150, 137-146 (2000) https://doi.org/10.1016/S0300-483X(00)00254-7
  34. Weiss, M. A., Hua, Q. X., Lynch, C. S., Frank, B. H. and Shoelson, S. E., Heteronuciear 2D NMR studies of an engineered insulin monomer: assignment and characterization of the receptor-binding surface by selective 2H and 13C labeling with application to protein design. Biochemistry, 30, 7373-7389 (1991) https://doi.org/10.1021/bi00244a004
  35. Wohaieb, S. A. and Godin, D. V., Alterations in free radical tissue-defense mechanisms in streptozocin- induced diabetes in rat. Effects of insulin treatment. Diabetes, 36, 1014-1018 (1987) https://doi.org/10.2337/diabetes.36.9.1014
  36. Young, I. S., Tate, S., Lightbody, J. H., McMaster, D. and Trimble, E. R, The effects of desferrioxamine and ascorbate on oxidative stress in the streptozotocin diabetic rat. Free Radic. Biol. Med., 18, 833-840 (1995) https://doi.org/10.1016/0891-5849(94)00202-U