한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제36권3호
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  • Pages.134-144
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  • 2003
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  • 0367-6315(pISSN)
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  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지

생물적 방제균 Pseudomonas chlororaphis O6의 길항 물질 생산 및 유기산 흡수에 관련된 dctA 유전자의 클로닝

Production of Antimicrobial Compounds and Cloning of a dctA Gene Related Uptake of Organic Acids from a Biocontrol Bacterium Pseudomonas Chlororaphis O6

  • 한송희 (전남대학교 농업생명과학대학 응용식물학부, 농업과학기술연구소) ;
  • 남효송 (전남대학교 농업생명과학대학 응용식물학부, 농업과학기술연구소) ;
  • 강범룡 (전남대학교 농업생명과학대학 응용식물학부, 농업과학기술연구소) ;
  • 김길용 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 구본성 (농촌진흥청 농업생명공학원) ;
  • 조백호 (전남대학교 농업생명과학대학 응용식물학부, 농업과학기술연구소) ;
  • 김영철 (전남대학교 농업생명과학대학 응용식물학부, 농업과학기술연구소)
  • Han, Song-Hee (Division of Applied Plant Science, Inst. of Agri. Sci. and Tech., College of Agriculture and Life Sciences, Chonnam National University) ;
  • Nam, Hyo-Song (Division of Applied Plant Science, Inst. of Agri. Sci. and Tech., College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kang, Beom-Ryong (Division of Applied Plant Science, Inst. of Agri. Sci. and Tech., College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Inst. of Agri. Sci. and Tech., College of Agriculture and Life Sciences, Chonnam National University) ;
  • Koo, Bon-Sung (National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Cho, Baik-Ho (Division of Applied Plant Science, Inst. of Agri. Sci. and Tech., College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Young-Cheol (Division of Applied Plant Science, Inst. of Agri. Sci. and Tech., College of Agriculture and Life Sciences, Chonnam National University)
  • 투고 : 2003.03.25
  • 심사 : 2003.05.16
  • 발행 : 2003.06.30
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초록

생물적 방제균인 Pseudomonas chlororaphis O6는 phenazine, protease와 HCN 등과 같은 여러 가지 2차 대사 산물들을 분비하여 식물병원균의 생육을 억제하였다. 또한 O6 균을 밀종자에 접종하였을 때, F. culmorum에 의한 뿌리 썩음병을 방제하는 효과가 있었다. 오이 뿌리 분비액내의 주 유기산은 fumaric acid, malic acid, benzoic acid, succinic acid 였고, 당으로는 glucose와 fructose가 검출되었으며, 유기산의 농도는 당의 농도보다 10배 정도 높았다. O6 균은 오이 뿌리 분비액을 영양원으로 하는 배지에서 생육하였다. 유기산을 흡수하는데 관여할 것으로 추정되는 O6 균의 dctA 유전자는 1,335 bp의 open reading frame을 가지고 있었으며, 444개의 아미노산으로 구성된 약 47 kD 의 pI가 8.2인 단백질을 암호화하였다. DctA 단백질은 10개의 putative trans-membrane domains를 가지고 있어, 세포막에 내재된 단백질로 추정되었다. 오이의 뿌리 분비액 중 유기산이 O6 균의 뿌리 정착에 중요한 물질로 작용한다면 본 연구에 의해 클로닝된 dctA 유전자는 식물 정착이나 생물적 방제균의 유용 형질의 발현을 연구하는데 중요한 유전자로 이용될 수 있을 것이다.

A rhizobacterium Pseudomonas cholororaphis O6 produced several secondary metabolites, such as phenazines, protease, and HCN that may be involved in inhibition of the growth of phytopathogenic fungi. In field study, P. chlororaphis O6 treatment on wheat seed suppressed root rot disease caused by Fusarium culmorum. The major organic acids of cucumber root exudates were fumaric acid, malic acid, benzoic acid, and succinic acid. Glucose and fructose were major monosaccharides in cucumber root exudates. The total amount of organic acids was ten times higher than that of the sugars. P. chlororaphis O6 grew well on cucumber root exudates. The dctA gene of P. chlororaphis O6 consisted of a 1,335 bp open reading frame with a deduced amino acid sequence of 444 residues, corresponding to a molecular size of about 47 kD and pI 8.2. The deduced dctA sequence has ten putative transmembrane domains, as expected of a membrane-embedded protein. Our results indicated that organic acids in cucumber root exudates may play an important role in providing nutrient source for root colonization of biological control bacteria, and the dctA gene of P. chlororaphis O6 may be an important bacterial trait that is involved in utilization of root exudates.

키워드

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