흰쥐에서 WGA-HRP와 pseudorabies virus를 이용한 정관의 신경로에 대한 연구

Neural pathway innervating ductus Deferens of rats by pseudorabies virus and WGA-HRP

  • 이창현 (우석대학교 한의과대학 해부학교실) ;
  • 정옥봉 (전북대학교 수의과대학 해부학교실) ;
  • 고병문 (우석대학교 한의과대학 해부학교실) ;
  • 이봉희 (제주대학교 의과대학 해부학교실) ;
  • 김수명 (대전대학교 한의과대학 해부학교실) ;
  • 김인식 (전북대학교 수의과대학 해부학교실) ;
  • 양홍현 (전북대학교 수의과대학 해부학교실)
  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Chung, Ok-Bong (Department of Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Ko, Byung-Moon (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Lee, Bong-Hee (Department of Anatomy, College of Medicine, Cheju National University) ;
  • Kim, Soo-Myung (Department of Anatomy, College of Oriental Medicine, Daejeon University) ;
  • Kim, In-Shik (Department of Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Yang, Hong-Hyun (Department of Anatomy, College of Veterinary Medicine, Chonbuk National University)
  • 심사 : 2003.03.15
  • 발행 : 2003.03.31

초록

This experimental studies was to investigate the location of PNS and CNS labeled neurons following injection of 2% WGA-HRP and pseudorabies virus (PRY), Bartha strain, into the ductus deferens of rats. After survival times 4-5 days following injection of 2% WGA-HRP and PRV, the rats were perfused, and their brain, spinal cord, sympathetic ganglia and spinal ganglia were frozen sectioned ($30{\mu}m$). These sections were stained by HRP histochemical and PRY inummohistochemical staining methods, and observed with light microscope. The results were as follows ; 1. The location of sympathetic ganglia projecting to the ductus deferens were observed in pelvic ganglion, inferior mesenteric ganglion and L1-6 lwnbar sympathetic ganglia. 2. The location of spinal ganglia projecting to the ductus deferens were observed in T13-L6 spinal ganglia. 3. The PRY labeled neurons projecting to the ductus deferens were observed in lateral spinal nucleus, lamina I, II and X of cervical segments. In thoracic segments, PRY labeled neurons were observed in dorsomedial part of lamina I, II and III, and dorsolateral part of lamina IV and V. Densely labeled neurons were observed in intermediolateral nucleus. In first lumbar segment, labeled neurons were observed in intermediolateral nucleus and dorsal commisural nucleus. In sixth lumbar segment and sacral segments, dense labeled neurons were observed in sacral parasympathetic nuc., lamina IX and X. 4. In the medulla oblongata, PRV labeled neurons projecting to the ductus deferens were observed in the trigeminal spinal nuc., A1 noradrenalin cells/C1 adrenalin cells/caudoventrolateral reticular nuc., rostroventrolateral reticular nuc., area postrema, nuc. tractus solitarius, raphe obscurus nuc., raphe pallidus nuc., raphe magnus nuc., parapyramidal nuc., lateral reticular nuc., gigantocellular reticular nuc.. 5. In the pons, PRV labeled neurons projecting to the ductus deferens were ohserved in parabrachial nuc., Kolliker-Fuse nuc., locus cooruleus, subcooruleus nuc. and AS noradrenalin cells. 6. In midbrain, PRV labeled neurons projecting to the ductus deferens were observed in periaqueductal gray substance, substantia nigra and dorsal raphe nuc.. 7. In the diencephalon, PRV labeled neurons projecting to the ductus deferens were observed in paraventricular hypahalamic nuc., lateral hypothalamic nuc., retrochiasmatic nuc. and ventromedial hypothalamic nuc.. 8. In cerebrum, PRV labeled neurons projecting to the ductus deferens were observed in area 1 of parietal cortex. These results suggest that WGA-HRP labeled neurons of the spinal cord projecting to the rat ductus deferens might be the first-order neurons related to the viscero-somatic sensory and sympathetic postganglionic neurons, and PRV labeled neurons of the brain and spinal cord may be the second and third-order neurons response to the movement of smooth muscles in ductus deferens. These PRV labeled neurons may be central autonomic center related to the integration and modulation of reflex control linked to the sensory and motor system monitaing the internal environment. These observations provide evidence for previously unknown projections from ductus deferens to spinal cord and brain which may be play an important neuroanatornical basic evidence in the regulation of ductus deferens function.

키워드

참고문헌

  1. Bell C. Autonomic nervous control of reproduction: circulatory and other factor. PhamacoI Rev, 24:657-736, 1972
  2. Hib J, Ponzio R, Vilar O. Contractility of the rat cauda epididymis and vas deferens during seminal emission. J Repro Fertil, 66:47-50, 1982
  3. Benson GS. Male sexual function: erection, emission, and ejaculation. The Physiology of Reproduction. E Knobil and JD Neil, eds. Raven Press, New York, pp. 1499-1506, 1994
  4. Dail WG, Trujillo 'D, Rosa D et al. Autonomic innervation of reproductive organ: analysis of the neurons whose axons project in the main penile nerve in the pelvic plexus of the mt. Anat Rec, 224:94-101, 1989
  5. Kihara K, Sato K, Oshima H. Sympathetic efferent pathways piojecting to the vas deferens. Microsc Res Tech, 42:398-408, 1998
  6. Kihara K, Sato K, Ando M et al. Control of bilateral seminal emissions from ejaculatory ducts by a lumbar splanchnic nerve. Am J Physiol, 265:R743-R748, 1993
  7. Knstenson K, Olsson Y. Retrograde axonal transport of protein. Brain Res, 29:363-365, 1971
  8. Keast JR. Location and peptide content of pelvic neuron supplying the muscle and lamina propria of th rat vas deferens. J Auton Nerv Syst, 40:1-12, 1992
  9. Kuypers HGJM, Ugolini G. Viruses as transneuronal tracers. TINS, 13:71-75, 1990
  10. Kristensson K, Nermesmo I, Persson L et al. Neuron to neuron transmission of hsrpes simplex virus. Transport of virus from skin to brain stem nuclei. J NeuroI Sci, 54: 149-156, 1982
  11. Martin X, Dolivo M. Neuronal and transneuronal tracing in the trigeminal system of the rat using the herpes virus suis. Brain Res, 273:253-276, 1983
  12. Strack AM, Sawyer WB, Hughes JH et al. A general pattern of CNS innervation of the sympathetic outflow demonstrated by transneuronal pseudorabies vintl infec-tion. Brain Res. 491:156-162, 1989
  13. Kucera P, Dolivo M, Coulon P et al. Pathways of early propagation of virulent and avirulent rabies straibs from the eye to the brain. J Virol, 55: 158-162, 1985
  14. Kihara K, de Groat WC. Sympathetic eferent pathway projecting bilaterally to vas deferens in the rat. Anat Rec, 248:291-299, 1997
  15. Janig W, McLachlan EM. Organization of lumbar spinal outflow to distal colon and pelvic organs. Physiol Rev, 67:1332-1404, 1987
  16. Matsuzaka H, Aoki H, Yeh KH et al. Experimental study of electric ejection reflex. Int J Impotence Res, 2:2-15, 1990
  17. Saxena PR. Effect of some drug on the response of the vas deferens and seminal vesicle to hypogastnc nerve stimulation in guinea pig. Phamacolosy, 3:220-228, 1970
  18. Kolbeck SC, Steers WD. Neural regulation of vas deferens in the rat. Am J PhysioI, 263: R331-R338, 1992
  19. Jewett M, Kong YS, Goldberg SD et al. Retio-peritoneal lympadenectomy fcx testis tumor with nerve sparing for ejaculation. J Urol, 139:1220-1224, 1988
  20. Costa M, Furness JB. Observation on the amtomy and amine histochemistry of the nerves and ganglia which supply the pelvic viscera and on the associated chromaffin tissue in the guinea-pig. Z Ana Entwickl-gesch, 140:85-108, 1973
  21. Dail WG, Evan AP, Eason HR. The major pelvic ganglion of the male rat: a histochemical and ultra-structural study. Cell Tissue Res, 159:49-62, 1975
  22. Keast JR. Patterns of co-existence of peptides and difference of nerve fiber types. CelI Tissue Res, 266:405-415, 1991
  23. Sato K, Kihara K. Spinal cord segments controlling the canine vas deferens and differentiation of primate sympathetic pathway to the vas deferens. Microsc Res Tech, 42; 390-397, 1998
  24. Sherlag AP, O'Brien DP, Graham SD. Use of limited retroperitoneal lymphadenectomy in nonseminomatous germ cell tumors. UroIogy, 33:355-357, 1989
  25. Spencer SE, Sawyer WB, Platt KB et al. CNS projections to pterygopalatine preganglionic neurons in the rat: a retrograde transneuronal viral cell body labelling study. Brain Res, 534:149-169, 1990
  26. Marson L, Platt KB, McKenna KE. Cential nervous system innervation of the penis as revealed by the transneuronal transport of the pseudorabies virus. Neuroscience, 55: 263-280, 1993
  27. Scott CK, Steers WD. Neural reguladon of the vas deferens in the rat: an electrophysiological analysis. Am J Physiol, 263:R331-R338, 1992
  28. McKenna KE. Chung SK, McVary KT. A model for the study of sexual function in the anesthetized male and female rat. Am J PhysioI, 261:R1276-R1285, 1991
  29. Nadelhaft I, McKenna KE. Sexual dimorphism in sympathetic preganglionic nsurons of the rat hypogastric narve. J Comp Neurol, 256:308-315, 1987
  30. Basbaum AI, Clanton CH, Field HL. Opiate and stimulus-producted analgesia, functional amtomy of a medullospinal pathway. Proc Nat Acad Sci, 73:4685-4688, 1976
  31. Du HJ. Medullary neurons with projections to lamina X of the rat as demonstrated by retrograde labelling after HRP microelectrophoresis. Brain Res, 505:135-140, 1989
  32. Loewy AD. Raphe pallidus and raphe obscurusprojections to the intermediolateral cell colurrm in the rat. Brain Res, 222:129-133, 1981
  33. Maison L, MsKennaKE A role for 5-hydroxytryptamine in descending inhibition of spinal sexual reflexes. Exp Brain Res, 88:313-320, 1992
  34. Schramm LP, Strack AM, Plaltt KB et al. Periperal and central pathways regulating the kidney: a study using pseudorabies virus. Brain Res. 616:251-262, 1991
  35. Holstege JC, Kuyper HGIM Brainstem projection to lumbar motoneurons in rat. Neuroscience, 21:345-367, 1987
  36. Hosoya Y. The distribution of spinal piojection neurons in the hypothalamus of the rat: studies with the HRP method. Exp Brain Res, 40:79-87, 1980
  37. Tanaka J, Kaba H, Saito H et al. Lateral hypothalamic area stimulation excites neurons in the region of fhe subfonical organ with efferent projecdons to the hypothalamic paraventricular nucleus in the rat. Brain Res, 379:200-203, 1986
  38. Andrezik JA. Chan-Palay V, Palay SL. The nucleus lesion prevent yawning and penile erection induced by apomorphine and oxytocin but not ACTH in rat. Brain Res, 421: 349-542, 1981
  39. Luiten PGM. The course of paraventricular hypo-thalamic effansnts to autonomic structures m medulla and spinal cord. Brain Res, 378:329-378, 1985