대한수의학회지 (Korean Journal of Veterinary Research)

  • 제43권1호
  • /
  • Pages.25-29
  • /
  • 2003
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

Microsatellite DNA형에 의한 더러브렛 말의 친자감정예

A case of parentage testing in the Thoroughbred horse by microsatellite DNA typing

  • Cho, Gil-Jae (Equine Blood Typing Laboratory, Korea Racing Association) ;
  • Yang, Young-Jin (Equine Blood Typing Laboratory, Korea Racing Association) ;
  • Kim, Bong-Hwan (College of Veterinary Medicine, Kyungpook National University)
  • 심사 : 2003.02.19
  • 발행 : 2003.03.31
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초록

This study was carried out to investigate a usefulness of the microsatellite DNA markers for parentage verification of Thoroughbred (TB) horses. 9 TB horses samples were genotyped for nine international minimum standard markers (AHT4, 5, ASB2, HMS3, 6, 7, HTG4, 10, and VHL20), and the additional panel of four markers, ASB17, CA425, LEX33, and TKY321. This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Foal I was excluded according to principles of Mendelian genetics in AHT4 (H/K), ASB2 (Q/Q), HMS3 (I/P), HTG4 (M/O), HTG1O (K/R), VHL20 (M/P), ASB17 (F/N), LEX33 (M/O), and TKY321 (G/I) markets. Foal II was excluded with markers AHT5 (K/M), ASB2 (M/N), HMS7 (N/N), HTG1O (K/K), VHL20 (I/I), ASB17 (F/F) and TKY321 (G/I). Foal III was excluded with markers AHT4 (O/O), AHT5 (K/K), ASB2 (M/R), HMS6 (M/P), HMS7 (O/O), HTG10 (R/S), VHL20 (L/M), and ASB17 (N/O). These results suggest that the present DNA typing is so useful for parentage verification of TB horses.

키워드

참고문헌

  1. Miura N. Blood typing service in light-breed horses. Jpn J Equine Sci, 4:187-190, 1994
  2. Bowling AT. Horse gewtics. CAB International, p. 82-96, 1996
  3. 조길재, 김봉환. 더러브렌 말의 혈액형에 관한 연구.대한수의학최지 40:683-689, 2000
  4. Natsuno Y. The present status of horse blood typing in Japan. J Anim Genet. 26:19-25, 1998
  5. Hasegawa T, Miura N. Advances in the equine (nolecular genedcs in Japan. Proc. 4th IEGMW. p. 13, 2001
  6. Kakai H, Nagata S, Kurosawa M, Microsatellite DNA testing for parentage verification of thoroughbreds. Pros 27th ISAG Conf Anim Genet, p. 90, 2000
  7. Tozlld T, Kakoi H, Mashima S, et al. Population study and validation of paternity testing for Thoroughhred horses by 15 microsatellite loci. J Vet Med Sci, 63:1191-1197, 2001
  8. Bozzini M, Fantin D, Ziegle JS, et al. Automated equine paternity testing. PE Applied Biosystenis. p. 1,1997
  9. Glowatzld-Mullis ML, Gaillard C, Wgger G, et al.MicrasatellilB-based parentage control in cattle. Anim Genet, 26:7-12, 1995
  10. Fwdhol M, Wnteio AK. Bfficient rsolution of parentage in dogs by amplification of microsateiltes. Anim Genet, 27:19-23, 1996
  11. 채영진, 이병천, 이황, 유전자감식에 의한 개에서의 친자감별, 한국임상수의학회, 15:274-278, 1998
  12. Ellegren H, Johansson M, Sandberg K, et al. Cloning of highly polymorphic microsatellites in the horse. Anim Genet, 23:133-142, 1992
  13. Alfod RB, Hammnond HA, Coto I, et al. Rapid and efficient resolution of parentage by amplification of short tandem repeats. American J Human Genet, 55, 190-195, 1994
  14. Mukoyama H. DNA analysis for individual identification of horses. Jpn J Equine Sci, 4:191-197, 1994
  15. Bowling AT. UC DAVIS book of horses. Mordecai Siegal, 1st ed. Haper Collins, p. 126, 1996
  16. Binns MM, Uolmes NG, Holliman AM The identifi-cation of polymorphic microsatellite loci in the horse and their use in thoroughbred parentage testing. Brit Vet J, 151:9-15, 1995
  17. Eggleston-Scott ML, Delvalle A, Dileanis S, et al. Characterization of a null allele at an equine mic-rosatellite locus. Anim Genet, 27: 89-90, 1996
  18. Eggleston-Scott ML, Delvalle A, Dileanis S, et al. A single base transversion in the flanking region of an equine microsatellite locus affects amplification of one allele. Anim Genet, 28:438-440, 1997