DOI QR코드

DOI QR Code

Hepatoprotection by Semisulcospira libertina against Acetaminophen-Induced Hepatic Injury in Mice

  • Jeon, Tae-Won (Institute for Drug Research, College of Pharmacy, Yeungnam University) ;
  • Lee, Young-Sun (Efficacy and Safety Research Center for Traditional Oriental Medicines, Daegu Haany University, Kyongbuk Technopark) ;
  • Kim, Hyo-Jung (College of Oriental Medicine, Daegu Haany University)
  • 발행 : 2003.09.01

초록

Recently, we reported (J Korean Soc Food Sci Nutr, 31(3): 516-520, 2002) that Semisulcospira libertina (Marsh Snail) pretreatment has a hepatoprotective effect on $CCl_4$-induced liver damage in rats. The purpose of this study was to investigate the possible mechanisms of hepatoprotection by S. libertina (SL) on liver injury induced by acetaminophen (AA). Male ICR mice were pretreated with dehydrated powder of SL once daily for three consecutive days, given a single toxic dose of AA (450 mg/kg) and liver function determined 24 h later. Liver damage was assessed by quantifying serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and sorbitol dehydrogenase (SDH) activities, and by measuring hepatic lipid peroxidation. To confirm possible mechanism(s), the content of hepatic glutathione (GSH) and gene expression of tumor necrosis factor a (TNF $\alpha$) mRNA by reverse transcription-polymerase chain reaction (RTPCR) were also measured. Pretreatment with SL dramatically lowered AA-elevated ALT, AST and SDH activities. SL pretreatment decreased AA-produced lipid peroxidation by 11% and restored the AA-depleted hepatic GSH by 27%. Furthermore, SL markedly suppressed the expression of TNF $\alpha$ mRNA induced by AA. Our findings revealed that the possible hepatoprotective mechanisms of SL could be attributed, at least in part, to the glutathione-mediated detoxification as well as the regulation of TNF $\alpha$ mRNA expression.

키워드

참고문헌

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  44. Inflammation v.17 Copper-dependent antioxidant defenses in inflammatory and autoimmune rheumatic diseases Miesel R;Zuber M https://doi.org/10.1007/BF00918991
  45. Inflammation v.19 Anti inflammatory effects of NADPH-oxidase inhibitors Miesel R;Sanocka D;Kurpisz M;Kroeger H https://doi.org/10.1007/BF01534392
  46. Drug Metab Dispos v.18 Effect of microsomal enzyme inducers on biliary and urinary excretion of acetaminophen metabolites in rats Gregus Z;Madhu C;Klasassen C
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  52. Int Hepatol Commun v.2 Enhanced expression of TNF α in patients with primary biliary cirrhosis Larrea E;Garcia N;Qian C;Civeira MP;Prieto J https://doi.org/10.1016/0928-4346(94)90003-5
  53. Crit Rev Toxicol v.29 Tumor necrosis factor α and toxicology Luster MI;Simeonova PP;Gallucci R;Matheson J https://doi.org/10.1080/10408449991349258
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  55. Toxicol Appl Pharmacol v.177 The role of tumor necrosis factor- α in liver toxicity, inflammation, and fibrosis induced by carbon tetrachloride Simeonova PP;Gallucci RM;Hulderman T;Wilson R;Kommineni C;Rao M;Luster MI https://doi.org/10.1006/taap.2001.9304
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  59. Methods of Enzymatic Analysis v.3 Gerlach U;Bergmeyer HU(ed.)
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  63. Toxicol Appl Pharmacol v.22 Sensitivity of several enzymes in detecting carbon tetrachloride-induced liver damage Kosrud GO;Grice HC;McLaughlin JM https://doi.org/10.1016/0041-008X(72)90255-4
  64. Vet Clin Pathol v.12 The mechanism relating to increases in plasma enzymes and isoenzymes in diseases of animals Boyd JW
  65. Biopharm Drug Dispos v.12 Drugs and the liver Sallie R;Tredger J;William R https://doi.org/10.1002/bdd.2510120403
  66. Planta Med v.53 A comparative study of the efficacy of Pavetta indica and Osbeckia octandra in the treatment of liver dysfunction Thabrew M;Joice P;Rajatissa W https://doi.org/10.1055/s-2006-962691
  67. Biochem Biophys Acta v.757 Glutathione-dependent protection by rat liver microsomal protein against lipid peroxidation Burk R https://doi.org/10.1016/0304-4165(83)90148-4
  68. Toxicol Appl Pharmacol v.133 Role of proinflammatory cytokines in acetaminophen hepatotoxicity Blazka ME;Wilmer JL;Holladay SD;Wilson RE;Luster MI https://doi.org/10.1006/taap.1995.1125
  69. Growth Factors v.1 Expression of tumor nectosis factor- α and transforming growth factor-β1 in acute liver injury Czaja MJ;Flanders KC;Biempica L;Klein C;Zern Ma;Weiner FR https://doi.org/10.3109/08977198908997998
  70. Toxicol Pathol v.24 Sinusoidal lining cells and hepatotoxicity Laskin DL https://doi.org/10.1177/019262339602400115
  71. Toxicol Lett v.98 Serum and liver concentrations of tumor necrosis factor α and interleukin-1β following administration of carbon tetrachloride to male rats DeCicco LA;Rikans LE;Tutor CG;Hornbrook KR https://doi.org/10.1016/S0378-4274(98)00110-6
  72. Toxicol Appl Pharmacol v.172 Distinct roles of tumor necrosis factor-α and nitric oxide in acute liver injury induced by carbon tetrachloride in mice Morio LA;Chiu H;Sprowles KA;Zhou P;Heck DE;Gordon MK;Laskin DL https://doi.org/10.1006/taap.2000.9133

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