Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Determination of Evodiae Fructus Alkaloids in O-Su-You-Tang by Spectrophotometric Method

흡광도 측정법에 의한 오수유탕 중 오수유 알칼로이드의 정량

  • Published : 2004.03.30
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Abstract

Abstract - The Evodiae Fructus is known for containing a number of indolquinazoline and quinoline type alkaloids. Evodiamine, evocarpine and rutaecarpine are the major constituents of alkaloids. These alkaloids were isolated and determined by forming complex compounds from Evodiae Fructus in O-Su-You-Tang. For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] compIεx compound ion. The absorbance of alkaloidal complex compounds in 1,2-dichloroethane solution was measured at 625 nm. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in O-Su-You-Tang.

Keywords

References

  1. Namba, T. (1993) The Encyclopedia of Wakan-Yaku(Traditional Sino-Japanese Medicines) with Color Picturevol. I, 233-235. Hoikusha, Osaka
  2. Kamikado, T., Murakoshi, S., and Tamura, S. (1978)Structure elucidation and synthesis of alkaloids isolated fromfruits of Evodia rutaecarpa. Agric. Biol. Chem. 42: 1515-1519 https://doi.org/10.1271/bbb1961.42.1515
  3. Kamikado, T., Chang, C. F., Murakoshi, S., Sakurai, A., andTamura, S. (1976) Isolation and structure elucidation of threequinolone alkaloids from Evodia rutaecarpa. Agric. Biol.Chem. 40: 605-609 https://doi.org/10.1271/bbb1961.40.605
  4. Tang, Y. Q., Feng, X. Z., and Huang, L. (1996) Quinolonealkaloids from Evodia rutaecarpa. Phytochemistry 43: 719-722 https://doi.org/10.1016/0031-9422(96)00304-4
  5. Kano, Y., Yasuda, M., Saito, K., and Komatsu, K. (1989)Simultaneous and quantitative analysis of alkaloids in EvodiaFruit. Shoyakugaku Zasshi 43: 339-342
  6. Peng Y. T., Shum A. Y., Tasi T. H,, Lin L. C., and Chen C.F. (1993) High-performance liquid chromatography of thequinazolinocarboline alkaloid dehydroevodiamine. J. Chromatogr. B 617: 87-93 https://doi.org/10.1016/0378-4347(93)80425-4
  7. Ko, H. C., Tsai, Y. H., Chou, C. J., Hsu, S. Y, Li, S. Y, andChen C. F. (1994) High-performance liquid chromatographicdetermination of rutaecarpine in rat plasma; application to apharmacokinetic study. J. Chromatogr. B 655: 27-31 https://doi.org/10.1016/S0378-4347(94)80128-2
  8. Jeng, K. F., Lin, Y. H., Lin, L. C., Chou, C. J., Tsai, T. H., andChen, C. F. (1995) High-performance liquid chromatographicdetermination of evodiamine in rat plasma; application to apharmacokinetic studies. J. Chromatogr. B 668: 343-345 https://doi.org/10.1016/0378-4347(95)00090-6
  9. Ueda, J., Moimna, N., Yamauchi, Y., Yoshie, F., and Ohsawa,K. (1996) A simultaneous determination of evodiamine andrutaecarpine in oriental pharmaceutical decoctions containing Evodia fruit by ion-pair high-performance liquid chromatography. Yakugaku Zasshi 116: 59-64 https://doi.org/10.1248/yakushi1947.116.1_59
  10. Philip, W. W. and Charles, G. D. (1951) Nature of the cobaltthiocyanate reaction. Anal. Chem. 23: 334-337 https://doi.org/10.1021/ac60050a027
  11. Leonard, I. K. and Elizabeth, G. (1950) Spectrophotometricstudies of cobalt(Ⅱ) thiocyanate complexes in organicsolvents. J. Am. Chem. Soc. 72: 5659-5662 https://doi.org/10.1021/ja01168a083
  12. S. E. Kim, D. K. Kim, T. Y. Shin, J. P. Lim, and D. O. Eum(2003) Isolation and Determination of Alkaloids in EvodiaeFrumctus by forming Complex Comound. Kor. J. Pharmacogn34: 206-209