Physical Mapping of rDNAs Using McFISH in Anemarrhena asphodeloides Bunge

지모에서 McFISH를 이용한 rDNAs의 물리지도 작성

  • Kim, Soo-Young (School of Biosciences & Biotechnology, Chungnam Natl. Univ.) ;
  • Choi, Hae-Woon (School of Biosciences & Biotechnology, Chungnam Natl. Univ.) ;
  • Bang, Jae-Wook (School of Biosciences & Biotechnology, Chungnam Natl. Univ.)
  • 김수영 (충남대학교 생명과학부) ;
  • 최혜운 (충남대학교 생명과학부) ;
  • 방재욱 (충남대학교 생명과학부)
  • Published : 2004.12.31

Abstract

Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.

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