Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Germ-line Transmission of Pseudotyped Retroviral Vector in Chicken

  • Heo, Y.T. (Animal Resources Research Center, Bio/Molecular Informatics Center, Konkuk University) ;
  • Kim, T. (Dept. of Physiology, Catholic Univ. of Daegu School of Medicine) ;
  • Lee, Y.M. (Dept. of Physiology, Catholic Univ. of Daegu School of Medicine) ;
  • Lee, C.K. (Dept. of Microbiology, Catholic Univ. of Daegu School of Medicine) ;
  • Kwon, M.S. (Dept. of Biology, Keimyung University) ;
  • Koo, B.C. (Dept. of Biology, Keimyung University) ;
  • Roh, K.S. (Dept. of Biology, Keimyung University) ;
  • Whang, K. (Dept. of Food Science and Technology, Keimyung University) ;
  • Han, D.W. (Dept. of Food Science and Technology, Keimyung University) ;
  • Chung, K.S. (Dept. of Food Science and Technology, Keimyung University) ;
  • Lee, H.T. (Animal Resources Research Center, Bio/Molecular Informatics Center, Konkuk University)
  • Received : 2003.06.04
  • Accepted : 2003.10.22
  • Published : 2004.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

Using MLV (murine leukemia virus)-based retrovirus vectors encapsidated with VSV-G (vesicular stomatitis virus G glycoprotein), we tried to make transgenic chickens carrying the transferred genes in their chromosomes. Twenty one days after virus injection beneath the blastoderms of unincubated chicken embryos (stage Ⅹ, at laying), DNA isolated from the hatched chicks were analyzed by PCR with two sets of primers specific for EGFP (enhanced green fluorescence protein) gene or $Neo^R$ (E. coli neomycin resistant) gene. Among sixty-seven embryos injected with retrovirus, four of them were identified to carry the EGFP genes in their genomes. Remarkably, one transgenic chick showed presence of the retrovirus vector sequences in all organs differentiated from one of endoderm, mesoderm, and ectoderm. Expression of EGFP gene was not detected, however, the stable germ line transmission of transgene was verified in spermatozoa from the founder chicken and 50% of $F_1$ progenies.

Keywords

References

  1. Battula, N. and H. M. Temin. 1977. Infectious DNA of spleen necrosis virus is integrated at a single site in the DNA of chronicallyinfected chicken fibroblasts. Proc. Natl. Acad. Sci. 74:281-285.
  2. Bosselman, R. A., R. Y. Hsu, M. J. Briskin, T. Boggs, S. Hu, M. Nicolson, L. M. Souza, J. A. Schultz, W. Rishell and R. G. Stewart. 1990. Transmission of exogenous genes into the chicken. J. Reprod. Fertil. Suppl. 41:183-95.
  3. Burns, J. C., T. Friedmann, W. Driver, M. Burrascano and J. K. Yee. 1993. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc. Natl. Acad. Sci. USA 90:8033- 8037.
  4. Chan, A. W. S., E. J. Homan, L. U. Ballou, J. C. Burns and R. D. Bremel. 1998. Transgenic cattle produced by reversetranscribed gene transfer in oocytes. Proc. Natl. Acad. Sci. USA 95:14028-14033.
  5. Chan, A. W. S., K. Y. Chong, C. Martinovich, C. Simerly and G. Schatten. 2001. Transgenic monkeys produced by retroviral gene transfer into mature oocytes. Science 291:309-312.
  6. Conlon, F. L., K. S. Barth and E. J. Robertson. 1991. A novel retrovirally induced embryonic lethal mutation in the mouse:assessment of the developmental fate of embryonic stem cells homozygous for the 413.d proviral integration. Development 111:969-981.
  7. Deeming, D. C., K. Rowlett and K. Simkiss. 1987. Physical influences on embryo development. J. Exp Zool Suppl. 1:341-5.
  8. Eyal-Giladi, H. 1984. The gradual establishment of cell commitments during the early stages of chick development. Cell Differ. 14:245-55. https://doi.org/10.1016/0045-6039(84)90013-7
  9. Eyal-Giladi, H. and S. Kochav. 1976. From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick. I. General morphology. Dev. Biol. 49:321-37.
  10. Etches, R. J. and A. M. Verrinder Gibbins. 1997. Strategies for the production of transgenic chicken. Methods Mol. Biol. 62:433-50
  11. Gaiano, N., M. Miguel, A. Amsterdam, K. Kawakami and N. Hopkins. 1996. Highly efficient germ-line transmission of proviral insertions in zebrafish. Proc. Natl. Acad. Sci. USA 93:7777-7782.
  12. Harvey, A. J., G. Speksnijder, L. R. Baugh, J. A. Morris and R. Ivarie. 2002. Expression of exogenous protein in the egg white of transgenic chickens. Nat. Biotechnol. 20:396-399.
  13. Kim, T., M. L. Leibfried-Rutledge and N. L. First. 1993. Gene transfer in bovine blastocysts using replication-defective retroviral vectors packaged with Gibbon ape leukemia virus envelopes. Mol. Reprod. Dev. 35:105-113.
  14. Kim, T., Y. M. Lee, H. T. Lee, Y. T. Heo, H.-C. Yom, M. S. Kwon, B. C. Koo, K. Whang and K. S. Roh. 2001. Expression of the E. coli LacZ gene in chicken embryos using replication defective retroviral vectors packaged with vesicular stomatitis virus G glycoprotein envelopes. Asian-Aust. J. Anim. Sci. 14:163-169.
  15. Lin, S., N. Gaiano, C. Patricia, J. C. Burns, T. Friedmann, J.-K. Yee and N. Hopkins. 1994. Integration and germ-line transmission of a pseudotyped vector in zebrafish. Science 265:666-668.
  16. Love, T., C. Gribbn, C. Mather and H. Sang. 1994. Transgenic bird by DNA microinjection. Bio/Technology 12:60-63.
  17. Lu, J. K., J. C. Burns and T. T. Chen. 1997. Pantropic retroviral vector integration, expression, and germline transmission in medaka (Oryzias latipes). Mol. Mar. Biol. Biotechnol. 6:289-295.
  18. Miller, A. D., J. V. Garcia, N. Von Suhr, C. M. Lynch, C. Wilson and M. V. Eiden. 1991. Construction and properties of retrovirus packaging cells based on Gibbon ape leukemia virus. J. Virol. 65:2220-2224.
  19. Naito, M., M. Watanabe, M. Kinutami, K. Nirasawa and T. Oishi. 1991. Production of quail-chick chimeras by blastoderm cell trans. Br. Poult. Sci. 32:79-86.
  20. Naito, M., A. Tajima, Y. Yasuda and T. Kuwana. 1994. Production of germline chimeric chickens, with high transmission rate of donor-derived gametes, produced by transfer of primordial germ cells. Mol. Reprod. Dev. 39:153-61. https://doi.org/10.1002/mrd.1080390206
  21. Perry, M. M. 1987. Nuclear events from fertilization to the early cleavage stage in the domestic foul (Gallus domesticus). J. Anat. 150:99-109.
  22. Perry, M. M., D. Morrice, S. Hettle and H. Sanf. 1991. Expression of exogenous DNA during the early development of the chick embryo. Roux's arch. Dev. Biol. 200:312-319.
  23. Perry, A. C., T. Wakayama, H. Kishikawa, T. Kasai, M. Okabe, Y. Toyoda and R. Yanagimachi. 1999. Mammalian transgenesis by intracytoplasmic sperm injection. Science. 284:1180-1183.
  24. Sang, H. and M. M. Perry. 1989. Episomal replication of cloned DNA injected into the fertilised ovum of the hen, Gallus domesticus. Mol. Reprod. Dev. 1:98-106
  25. Savva, D., N. Page, L. Vick and K. Simkiss. 1991. Detection of foreign DNA in transgenic chicken embryos using the polymerase chain reaction. Res. Vet. Sci. 50:13-133.
  26. Shuman, R. M. and R. N. Shoffner. 1986. Gene transfer by avian retroviruses. Poult. Sci. 65:1437-1444.
  27. Thoraval, P., M. Afanassiefff, F. L. Cosset, F. Lasserre, G. Verdier, F. Coudert and G. Dambrine. 1995. Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leucosis virus-based vectors. Transgenic Res. 4:369-377.
  28. Tsunekawa, N., M. Naito, Y. Sakai, T. Nishida and T. Noce. 2000. Isolation of chicken vasa homolog gene and tracing the origin of primordial germ cells. Development 127:2741-50.
  29. Vick, L., Y. Li and K. Simkiss. 1993. Transgenic bird from transformed primordial germ cells. Proc. R. Soc. Lond. 251:179-182.

Cited by

  1. Avian Somitic Cell Chimeras Using Surrogate Eggshell Technology vol.21, pp.6, 2004, https://doi.org/10.5713/ajas.2008.70545