Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Expression of the Antioxidant Enzyme and Apoptosis Genes in In vitro Maturation/In vitro Fertilization Porcine Embryos

  • Jang, H.Y. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Kong, H.S. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Lee, S.S. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Choi, K.D. (East West Kidney Disease Research Institute, School of Medicine, Kyung Hee University) ;
  • Jeon, G.J. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Yang, B.K. (Division of Animal Resources Science, Kangwon National University) ;
  • Lee, C.K. (School of Agricultural Biotechnology, Xenotransplantation Research Center, Seoul National University) ;
  • Lee, H.K (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University)
  • Received : 2003.05.14
  • Accepted : 2003.09.06
  • Published : 2004.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was aimed at testing the gene expression of antioxidant enzymes and apoptosis genes for in vitro culture in porcine embryos produced by in vitro maturation/in vitro fertilization (IVM/IVF). Pocine preimplantation embryos obtainted from IVM/IVF can be successfully culture in vitro, but they are delayed or stop to develop at specific developmental stage. Many factors such as reactive oxygen species and apoptosis in an IVM/IVF system followed by in vitro culture influence the rate of production of viable blastocysts. Porcine embryos derived from IVM/IVF were cultured in the atmosphere of 5% $CO_2$ and 20% $O_2$ at $38.5^{\circ}C$ in NCSU23 medium. The patterns of gene expression for antioxidant enzymes and apoptosis genes during in vitro culture in pocine IVM/IVF embryos were examined by the modified semi-quantitative single cell reverse transcriptase-polymerase chain reaction (RT-PCR). Porcine embryos produced by in vitro procedures were expressed mRNAs for CuZn-SOD, GAPDH and GPX, whereas transcripts for Mn-SOD and catalase were not detected at any developmental stages. Expression of caspase-3 mRNA was detected at 2 cell, 8 cell 16 cell and blastocyst, but p53 mRNA was not detected at any stages. The fas transcripts was only detected in blastocyst stage. These results suggest that various antioxidant enzymes and apoptosis genes play crucial roles in vitro culture of porcine IVM/IVF embryos.

Keywords

References

  1. Betts, D. H. and W. A. King. 2001. Genetic regulation of embryo death and senescence. Theriogenology 55:171-191.
  2. Brison, D. R. and R. M. Schultz. 1997. Apoptopsis during mouse blastocyst formation: evidence for a role for survival factors including transf orming growth factor alpha. Bio. Reprod. 56:1088-1096.
  3. Byrne, A. T., J. Southgate, D. R. Brison and H. J. Leese. 1999. Analysis of apoptosis in the preimplantation bovine embryo using TUNNEL. J. Reprod. Fert. 117:97-105.
  4. Chandra, J., A. Samali and S. Orrenius. 2000. Triggering and modulation of apoptosis by oxidative stress Free. Radic. Biol. Med. 29:323-333.
  5. Exley, G. E., C. Tang, A. S. McElhinny and C. M. Warner. 1999. Expression of caspase and BCL-2 apoptotic family members in mouse preimplantation embryo. Bio. Reprod. 61:231-239.
  6. Goto, Y. Y. Noda, K. Narimoto, Y. Umaoka and T. Mori. 1992. Oxidative stress on mouse embryo development in vitro. Free Radical Biol. Med. 13:673-679.
  7. Halliwell, B. and J. M. C. Gutteridge. 1989. Free Radicals in Biology and Medicine, 2nd ed. Oxford University press.
  8. Handyside, A. H. and S. Hunter. 1986. Cell division and death in the mouse blastocyst before implantation. Arch. Develop. Bio. 195:519-526. https://doi.org/10.1007/BF00375893
  9. Hardy, K. 1999. Apoptosis in the human embryo. Reviews of Reproduction 4:125-134.
  10. Hardy, K. S. Spanos, D. Becker, P. Lannelli, R. M. Winston and J. Stark. 2001. From cell death to embryo arrest: mathematical models of human preimplantation embryo development. Proceed. Nal’t Acad. Sci. 98:1655-1660.
  11. Hardy, K. 1997. Cell death in the mammalian blastocyst. Mol. Hum. Reprod. 3:919-925.
  12. Johnson, S. E., J. L. Rothstein and B. B. Knowles. 1994. Expression of epidermal growth factor family gene members in early mouse development. Dev. Dyn. 201(3):216-226.
  13. Jorhson, M. H. and M. H. Nasr-Esfahani. 1994. Radical solutions and culture problems: could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro. Bioessays 16:31-38.
  14. Li, P. F. and R. Harsdorf. 1999. p53 regulates mitochondrial membrane potential through reactive oxygen species and induces cytochrome c-independent apoptosis blocked by bcl-2. EMBO J. 18:6027-6036.
  15. Lee, C. K. and J. A. Piedrahita. 2003. Transgenesis and germ cell engineering in domestic animals. Asian-Aust. J. Anim. Sci. 16:910-927.
  16. Maas, D. H., B. T. Storey and L. Mastroianni, Jr. 1976. Oxygen tension in the oviduct of the rhesus monkey. Fertil. Steril. 27:1312-1317
  17. Majima, H. J., T. D. Oberley, K. Furukawa, M. P. Mattson, H. J. Yen, L. I. Szweda and D. K. St. Clair. 1998. Prevention of mitochondrial injury by manganesium superoxide dismutase reveals a primary mechanism for alkaline-induced cell death. J. Bio1. Chem. 273:8217-8224.
  18. Matwee, C., D. H. Betts and W. M. King. 2000. Apoptosis in the early bovine embryo. Zygote 8:57-68.
  19. Miyashita, T. and J. C. Reed. 1995. Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 80:293-299.
  20. Muller, M., S. Wilder, D. Bannasch, D. Israeli, K. Lehlbach, M. Liwever, S. L. Friedman, P. R. Galle, W. Stremmel, M. Oren and P. H. Krammer. 1998. p53 activates the CD95 APO-1/Fas gene in response to DNA damage by anticancer drugs. J. Exp. Med. 188:2033-2045.
  21. Nasr-Esfahani, M. H. and M. H. Johnson. 1992. Quantitative analysis of cellular glutathione in early preimplantation mouse embryos developing in vivo and in vitro. Hum. Reprod. 7(9):1281-90
  22. Noda, Y., H. Matsumoto, Y. Umaoka, K. Tatsumi, J. Kishi and T. Mori. 1991. Involvement of superoxide radicals in the mouse two-cell block. Mol. Reprod. Dev. 28(4):356-60. https://doi.org/10.1002/mrd.1080280408
  23. Park, K. E., C. K. Park, C. I. Kim, H. T. Cheong, D. H. Park and B. K. Yong. 2001. Effect of nitric oxide compounds on the development of porcine IVM/IVF embryos. Kor. J. Anim. Reprod. 25:63-69.
  24. Petters, R. M. and K. D. Wells. 1993. Culture of pig embryos. J. Reprod. Fertil. 48(Suppl):61-73.
  25. Stridh, H., M. Kimland, D. P. Jones, S. Orrenius and M. B.Hampton. 1998. Cytochrome c release and caspase activation in hydrogen peroxide- and tributyltinind hampton uced apoptosis. FEBS Lett. 429:351-355.
  26. Suhara, T., Fukyo, T. Sugimoto, S. Morimoto, T. Nakahashi, S. Hata, M. Shimizu and T. Ogihara. 1998. Hydrogen peroxide induces up-regulation of fas in human endothelial cell. J. Immunol. 160:4042-4047.
  27. Uberti, D., E. Yavin, S. Gil, K. R. Ayasola, N. Goldfinger and Rotter. 1999. Hydrogen peroxide induces nuclear translocation of p53 and apoptosis in cells of oligodendroglia origin. Mol. Brain Res. 65:167-175.
  28. Wang, W. H., L. R. Abeydeera, T. C. Cantley and B. N. Day. 1997. Effects of ooxyte maturation media on development of pig embryos produced by in vitro fertilization. J. Reprod. Fert. 111, 101-108.
  29. Weil, M., M. D. Jacobson, H. S. T. J. Davies, R. L. Gardner, K. D. Raff and M. C. N. Raff. 1996. Constitutive expression of the machinery for programmed cell death. J. Cell Biol. 133:1053-1059. https://doi.org/10.1083/jcb.133.5.1053

Cited by

  1. Effects of Taurine on Sperm Characteristics during In vitro Storage of Boar Semen vol.19, pp.11, 2004, https://doi.org/10.5713/ajas.2006.1561
  2. Addition of Macromolecules to PZM-3 Culture Medium on the Development and Hatching of In vitro Porcine Embryos vol.20, pp.12, 2004, https://doi.org/10.5713/ajas.2007.1820
  3. Effects of IGF-I and EGF Supplemented to PZM3 Culture Medium on the Development of Porcine Embryos In vitro vol.22, pp.8, 2009, https://doi.org/10.5713/ajas.2009.90051
  4. Utilization of Rosmarinic and Ascorbic Acids for Maturation Culture Media in Order to Increase Sow Oocyte Quality Prior to IVF vol.26, pp.23, 2021, https://doi.org/10.3390/molecules26237215