Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Analysis of genetic diversity for cattle parentage testing using microsatellite markers

소의 친자감정을 위한 Microsatellite markers의 유전적 다양성 분석

  • Cho, Gil-jae (Laboratory of Equine Genetics, Korea Racing Association) ;
  • Yang, Young-jin (Laboratory of Equine Genetics, Korea Racing Association) ;
  • Lee, Kil-wang (Department of Animal Science, Miryang National University)
  • 조길재 (한국마사회 유전자검사실) ;
  • 양영진 (한국마사회 유전자검사실) ;
  • 이길왕 (밀양대학교 동물자원학과)
  • Accepted : 2004.04.17
  • Published : 2004.06.30
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Abstract

The objective of present study was to ascertain genetic diversity for cattle parentage testing. A total of 59 random cattle samples(29 Korean native cattle and 30 dairy cows) were genotyped by using 11 microsatellite loci(BM1824, BM2113, ETH10, ETH225, EH3, INRA23, SPS115, TGLA122, TGLA227, TGLA53, and TGLA126). This method consisted of multiplexing PCR procedure and showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. The number of alleles per locus varied from 5 to 11 with a mean value of 6.73 in the Korean native cattle(KNC), 4 to 9 with a mean of 5.91 in dairy cows(DC). Expected heterozygosity was ranged 0.534~0.855(mean 0.732), 0.370~0.866(mean 0.692) in the KNC and DC, respectively. PIC value was ranged 0.485~0.821(mean 0.684), 0.336~0.834(mean 0.640) in the KNC and DC, respectively. Of the 11 markers, 7 markers(ETH10, EH3, INRA23, SPS115, TGLA122, TGLA227, TGLA53) and 3 markers(INRA23, TGLA227, TGLA53) have relatively high PIC value (>0.7) in the KNC and DC, respectively. The total exclusion probability of 11 microsatellite loci was 0.9997 and 0.9991 in the KNC and DC, respectively. These results present basic information for developing a system for parentage verification and individual identification in the KNC and DC.

Keywords

References

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