Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Korean Traditional Tea Materials on Minerals Content and Histological Changes in Pb-Administered Rats

전통음료 소재가 납투여한 흰쥐의 체내 무기질 함량 및 간과 신장조직의 헝태학적 변화에 미치는 영향

  • 조수영 (영남대학교 식품영양학과) ;
  • 김명주 (대구산업정보대학 식품영양과) ;
  • 이미경 (경북대학교 식품생물산업연구소) ;
  • 박은미 (대구한의대학교 한방생명자원 특화센터) ;
  • 장주연 (영남대학교 식품영양학과) ;
  • 최정목 (대전보건대학) ;
  • 김덕진 (대구대학교 식품생명화학공학부)
  • Published : 2004.02.01
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Abstract

This study was designed to investigate the effect of Korean traditional tea materials on lead (Pb) accumulation and histological changes in Pb-administered rats. Male rats were divided into six groups: a normal, Pb, and Pb-water extract of Korean traditional tea materials (green tea: GT, persimmon leaf: PL, safflower seed: SS, Eucommia ulmoides: EU) groups. Pb (25 mg/kg BW) was orally administered once a day for 4 weeks. The extract of GT, PL, SS, and EU were administered based on 1.26 g of raw traditional tea materials/kg Bw/day. Pb contents of serum and liver were significantly higher in the Pb group than in the normal group. However the water extracts of GT, PL, SS, and EU administration lowered Pb contents of serum and liver in the Pb-administered rats, respectively. Serum Zn content was significantly higher in the Pb group than in the normal group, whereas, Cu and Fe contents were lower in the Pb group. Hepatic Ca content was significantly lower in the Pb group compared to the normal group. In the present study, water extract of Korean traditional tea materials administration effectively improved Cu and Fe contents of serum and liver. Infiltration of the local inflammation was found in Pb group, whereas water extracts of Korean traditional tea materials administration attenuated histological changes of tissues.

전통음료의 소재로 애용되는 녹차, 감잎 홍화 및 두충의 열수추출물이 납투여된 흰쥐의 체내 무기질 함량 및 간과 신장조직의 형태학적 변화에 미치는 영향을 구명하기 위하여 체중kg당25mg의 납을 매주 1회 경구투여하였다. 녹차잎, 감잎, 홍화씨 및 두충수피의 열수추출물은 매일 일정시간에 체중 kg당 1.26 g수준이 되도록 4주간 경구투여하였다. 전통음료 소재의 열수추출물 급여시 납투여로 인한 혈청과 간조직의 납축적 억제와 혈청 중의 철과 구리 함량 증가에 효과적인 것을 관찰할 수 있었다. 납투여시 혈청 중 포도당과 알부민 함량은 유의적으로 감소되었으며 크레아티닌과 빌리루빈 할량은 유의 적으로 증가되었다. 반면, 실험식이 급여시 이들 혈청 중 성분함량의 회복을 보였다. 또한 간조직 및 신장조직의 형태학적 변화는 납단독투여군에서 괴사성 소견 및 염증세포의 출현이 관찰되었으나 전통음료 소재의 열수추출물 급여시 조직학적 변화를 완화시키는 것으로 나타났다.

Keywords

References

  1. Alice YS, Charles K. 1989. Lead distribution in rats repeatedly treated with low dose of lead acetate. Environ Res 48: 238-247. https://doi.org/10.1016/S0013-9351(89)80037-4
  2. McDonell LR. 1992. Minerals in animal and human nutrition. Academic Press Inc., San Diego. p 359-361.
  3. Quarterman J, Morrison JN. 1975. The effect of dietary calcium and phosphorus on the retention and excretion of lead in rats. Br J Nutr 34: 351-362.
  4. Kim MK, Lee HY. 1988. Effects of dietary cadmium and protein levels on the body protein metabolism and cadmium toxicity in growing rats. J Korean Nutr 21: 410-420.
  5. Kim MK, Lee HY. 1989. Detoxification study with different dietary protein levels and detoxifying periods in lead poisoned rats. J Korean Nutr 22: 185-193.
  6. Choi SI, Lee JH. 1994. Effect of green tea beverage for the removal of cadmium and lead by animal experiments. Korean J Food Soci Techol 26: 745-749.
  7. Jo CY, Choi HJ, Ba DK, Son JH, Park MH, Woo HS, An BJ, Bae MJ, Choi C. 2000. Removal effect of cadmium by polyphenol compound extracted from persimmon leaves (Diospyros kaki folium). J Korean Soc Agric Chem Biotechnol 43: 213-217.
  8. Park KH, Kwon YD, Park MA, Park BJ. 2001. A study on the adsorption kinetics of heavy metals, Cd (II), Cu (II) and Zn (II) ions by the persimmon leaves. J Korea Soci Environ Analy 4: 159-167.
  9. Lee IK, Kim JG. 2000. Effects of extract of Eucommia ulmoides Oliv on the reduction of lead and cadmium in organs of rats. J Korean Public Health Assoc 26: 22-28.
  10. Hwan SY. 1998. A study on detoxification effects of Flos Carthami against cadmium poisoning in rats. MS Thesis. Wonkwang University. p 1-17.
  11. Mahaffey KR. 1981. Nutritional factors in lead poisoning. Nut Rev 39: 353-362. https://doi.org/10.1111/j.1753-4887.1981.tb06712.x
  12. Report of American Institute of Nutrition. 1977. Ad Hoc committee on standards for nutritional studies. J Nutr 107: 1340-1348.
  13. Thompson RH, Blanchflow WJ. 1984. Wat-ashing appratus to prepare biological materials for atomic absorption spectrometry. Lab Proc 20: 859-865.
  14. Conard ME, Barton JC. 1987. Factors affecting the absorption and excretion of lead in the rats. Gastroenterology 74: 731-740.
  15. Kim JS, Kim MK. 1987. Metabolic changes in growing rats fed diets with different levels of lead and lipid. Korean J Nutr 20: 225-236.
  16. Singh B, Dhawan D, Mangal PC, Chand B, Singh N, Trehan PN. 1994. Combined action of lead and lithium on essential and nonessential elements in rat blood. Bio Trac Elem Res 46: 15-28. https://doi.org/10.1007/BF02790065
  17. Barltrop D, Khoo HE. 1975. The influence of nutritional factors on lead absorption. Postgrad Med J 51: 795-800. https://doi.org/10.1136/pgmj.51.601.795
  18. Kim MJ, Cho SY, Jang JY, Park JY, Park EM, Lee MK, Kim DJ. 2003. Effect of water extract of green tea, persimmon leaf and safflower seed on heme synthesis and erythrocyte antioxidant enzyme activities in lead-administered rats. J Korean Soc Food Sci Nutr 32: 191-196. https://doi.org/10.3746/jkfn.2003.32.2.191
  19. Klauder DS, Petering HG. 1977. Anemia of lead intoxication: a role for copper. J Nutr 107: 1779-1785.
  20. Klauder DS, Murthy L, Petering HG. 1972. Effect of dietary intake of lead acetate on copper metabolism in male rat. Trace subst Environ Health-6 Proc Univ Mo Conf 6: 131-136.
  21. Dave V, Vitarella D, Aschner JL, Fletcher P, Kimelberg HK, Aschner M. 1993. Lead increased inositol 1,4,5-triphosphate levels but does not interfere with calcium transients in primary rat astrocytes. Brain Res 618: 9-18. https://doi.org/10.1016/0006-8993(93)90422-J
  22. Filkins JP. 1973. Hypoglycemia and depressed hepatic gluconeogenesis during endotoxicosis in lead-sensitized rats. Proc Soc Exp Biol Med 142: 915-918. https://doi.org/10.3181/00379727-142-37143
  23. Karmakar N, Saxena R, Anand S. 1986. Histopathological changes induced in rats tissues by oral intake of lead acetate. Environ Res 41: 23-28. https://doi.org/10.1016/S0013-9351(86)80164-5

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