Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Increased Expression of aac(3)II by Tn3 in Gentamicin - Resistant Bacteria Isolated from Hospital Sewage

병원하수로부터 분리한 Gentamicin 저항성 세균에서 Tn3에 의한 aac(3)II의 발현 증가

  • Published : 2004.03.01
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Abstract

We tested gentamicin - resistant bacteria isolated from hospital sewage to confirm the presence of aac(3)II encoding aminoglycoside- (3)-N- acetyltransferase by dot-blot hybridization. A probe from the internal fragment of aac(3)II was hybridized to DNA from 41 % (39/95) of gentamicin resistant isolates. PCR was performed with primers from aac(3)II and Tn3. Of 39 strains, 13 strains had Tn3-aac(3)II structure. Minimal inhibitory concentration (MIC) test demonstrated that 18 strains containing Tn3-aac(3)II showed higher resistance to gentamicin than those of other strains. Thirteen strains were identified as 5 Escherichia coli, 3 Acinetobacter johnsonii, 2 Enterobacter agglomerans, 2 Micrococcus luteus, and 1 Pseudomonas facilis. These results suggest that gentamicin-resistant determinant of Tn3-aac(3)II structure was widely distributed in the gentamicin-resistant bacteria.

Gentamicin에 저항성을 나타내는 세균을 병원 하수로부터 분리하여 aminoglycoside-(3)- N-acetyltransferase를 암호화하는 유전자인 aac(3)II의 존재여부를 dot-blot hybridization으로 조사하였다. aac(3)II유전자의 일부를 탐침으로 사용한 결과 gentamicin저항성 세균의 41% (39/95)가 이 유전자를 가지고 있었다. aac(3)II와 Tn3에서 각각 설계된 primer를 사용한 PCR 결과 aac(3)II를 가지고 있는 39개 균주 중 13개 균주가 TnS-aac(3)II구조를 가지고 있음을 알 수 있었다. aac(3)II의 상류에 Tn3를 가지고 있는 13개 균주의 gentamicin에 대한 최소억제농도는 가지고 있지 않은 균주에 비해 상대적으로 높았다. 13개 균주를 동정한 결과 5개는 Eschelichia coli, 3개는 Acinetobacter johnsonii, Enterobacter agglomerans와 Micrococcus luteus가 각각 2개, 그리고 1개의 Pseudomonas facilis로 동정되었다. 이러한 결과들은 Tn3-aac(3)II구조가 gentamicin 저항성 세균들에서 널리 분포하고 있음을 말해준다.

Keywords

References

  1. Scand. J. Infect. Dis. v.20 Aminoglycoside resistance patterns in Turkey Akalin,H.E.;M.Torun;R.Alacam https://doi.org/10.3109/00365548809032438
  2. Eur. J. Epidemiol v.9 Molecular epidemiology of two genes encoding 3-N aminoglycoside acetyltransferases AAC(3)I and AAC(3)II among gram-negative bacteria from a Spanish hospital Alvarez,M.;M.C.Mendoza
  3. Appl. Environ. Microbiol. v.60 Distribution of tetracycline resistance determinants among gram-negative bacteria isolated from polluted and unpolluted marine sediments Andersen,S.R.;R.A.Sanda
  4. Current protocols in molecular biology(4th ed.) Ausubel,R.M.;R.Brent;R.E.Kingston;D.D.Moore;J.G.Seidman;J.A.Smith;K.STruhl
  5. J. Bacteriol v.170 Evidence for natural gene transfer from gram-positive cocci to Escheerichia coli Brisson-Noel,A.;M.Arthur;P.Courvalin https://doi.org/10.1128/jb.170.4.1739-1745.1988
  6. J. Bacteriol v.175 Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli Cohen,S.P.;H.Hachler;S.B.Levy https://doi.org/10.1128/jb.175.5.1484-1492.1993
  7. Clin. Infect. Dis. v.31 Aminoglycoside resistance in Enterococci Chow,J.W. https://doi.org/10.1086/313949
  8. Bergey's manual of system-atic bacteriology David,R.B.;W.C.Richard
  9. Microbiol. Rev. v.47 Plasmid determined resistance to antimicrobial drugs and toxic metal ions in bacteria Foster,T.J.
  10. Eur. J. Clin Microbiol. Infect. Dis. v.20 Emergence of high-level gentamicin resistance in Group G Streptococci Faibis,F.A.Fiare;M.C.Demachy https://doi.org/10.1007/s100960100627
  11. Antimicrob. Agents Chemother v.43 High-level aminoglycoside resistance in the β-hemolytic group G Streptococcus isolate BM2721 Galimand,M.;T.Lambert;G.Gebaud;P.Couvalin
  12. J. Bacteriol v.173 marA, a regulated locus which cotrols expression of chromosomal multiple antibiotic resistance in Escherichia coli Hachler,H.;S.P.Cohen;S.B.Levy https://doi.org/10.1128/jb.173.17.5532-5538.1991
  13. Kor. J. Microbiol. v.33 Occurrence of Tn3 sequence upstream of aacC2 gene in gentamicin resistance R plasmids Han,H.S.N.D.Kim;Y.J.Lee;H.Y.Lee;J.S.Jung
  14. J. Antimicrob. Chemother v.31 Determination of aminoglycoside resistance mechanisms of Enterobacteriaceae isolated from a hospital in Hong Kong with antibiogram and genotyping Ho,B.S.W.;R.S.Hare;K.J.Shaw;G.H.Miller;M.H.Ng. https://doi.org/10.1093/jac/31.1.174
  15. Antimicrob. Agents Chemother v.35 Characterization fo the gentamicin resistance transponson Tn5281 from Enterococcus faecallis and comparison to staphylococcal transposons Tn4001 and Tn4031 Holden-Christian,S.K.;B.E>Murray https://doi.org/10.1128/AAC.35.6.1147
  16. Antimicrob. Agents Chemother v.40 Chromosomal gentamicin resistance transposon Tn3706 in Streptococcus agalactiae B128 Horau,T.;G.de.Cespedes;P.Trieu-Cuot
  17. Mol. Cells v.8 Incrased expression of the gentamicin resistance gene by a Tn3 sequence Iocated at the upstram region Jung,J.S.;H.Y.Lee;J.H.Chung
  18. Soil Biol. Biochem. v.16 Mercury resistance among soil bacteria: ecology and transferability of genes encoding resistance Kelley,W.J.;D.C.Reanney https://doi.org/10.1016/0038-0717(84)90116-0
  19. J. Infect. Chemother v.9 High-level gentamicin-resistant isolates of oral streptococci and Aerococcus from blood specimens Kobayashi,I.;A.Kanayama;K.Matsuzaki,M.;Nishida;N.Nakatogawa;A.Kaneko https://doi.org/10.1007/s10156-002-0221-7
  20. Antibiotics: a multidisciplinary approach Lancini,G.;F.Parenti;G.G.Gallo
  21. Kor. J. Microbiol. v.36 Molecular biolgoical detection of the genes encoding aminoglycoside acetyltransferases and aerolysin in water samples from Juam lake Lee,Y.J.;H.S.Han;J.S.Jung
  22. Kor. J. Microbiol. v.36 Distribution of genes coding for aminoglycoside acetyltransferases in gentamicin resistance baxteria isolated from aquatic environment Lee,Y.J.;H.S.Han;C.N.Seong;H.Y.Lee;J.S.Jung
  23. Mol. Gen. Genet. v.193 Tn4001: a gentamicin and Kanamycin resistance transposon in Staphylococcus aureus Lyon,B.R.;J.W.May;R.A.Skurray https://doi.org/10.1007/BF00382099
  24. Microbiol. Rev. v.51 Antrimicrobial resistance of Staphylococcus aureus: genetic basis Lyon,B.R.;R.A.Skurray
  25. Eur. J. Clin. Microbiol v.14 Aminoglycosides: the complex problem of antibiotic mechanisms and clinical applications Montie,T.;P.Patamasucon https://doi.org/10.1007/BF02111863
  26. FEMS Microbiol. Lett. v.93 Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC12201 to Staphylococcus aureus Noble,W.C.;Z.Virani;R.G.A.Gree https://doi.org/10.1111/j.1574-6968.1992.tb05089.x
  27. Appl. Environ. Microbiol. v.55 Turnover of extracellular DNA in eutrophic and oligotrophic freshwater environment of southwest Florida Paul,J.H.;W.H.Jeffrey;A.W.David;M.F.Deflaun;L.H.Cazares
  28. J. Bacteriol. v.176 Identification and characterization of genes involved in excision of the Lactococcus lactis conjugative transposon Rauch,P.J.;W.M.De Vos https://doi.org/10.1128/jb.176.8.2165-2171.1994
  29. Antimicrob. Agents Chemother v.39 Tn5384, a composite enterococcal mobile element conferring resistance to erythromycin and gentamicin whose ends are directly repeated copies of IS256 Rice,L.B.;L.L.Carias;S.H.Marshall https://doi.org/10.1128/AAC.39.5.1147
  30. Gene transfer in the environment The aquatic environment: consideration of horizontal gene transmission in a diversified habitat Saye,D.J.;R.Miller;S.B.Levy(ed.);R.V.Miller(ed.)
  31. Eur. J. Clin. Microbiol. Infect. Dis. v.18 Prevalence of aminoglycoside resistance in 20 European university hospitals participarting in the European sentry antimicrobial surveillance programme Schmitz,F.J.;J.Verhoef;A.C.Fluit;the Sentry participants group https://doi.org/10.1007/s100960050310
  32. Microbiol. Rev. v.57 Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes Show,K.J.;P.N.Rater;R.S.Hare;G.H.Miller
  33. J. Antimicrob. Chemother v.31 The use of a DNA probe and PCR to examine the distribution of the aac(6')-Ic gene in Serratia marcescens and other Gram-negative bacteria Snelling,A.M.;P.M.Hawkey;J.Heritage;P.Downey;P.M.Bennett;B.Holmes https://doi.org/10.1093/jac/31.6.841
  34. Antimicrob. Agents Chemother v.38 Tn924, a chromosome-bome transposon encoding high-level gentamicin resistance in Enterococcus faecalis Thal,L.A.;J.W.Chaw;D.B.Clewell;M.J.Zervos https://doi.org/10.1128/AAC.38.5.1152
  35. Antimicrob. Agents Chemother v.33 Mobility of gentamicin resistance genes from staphylococci isolated in the United States: identification of Tn4031, a gentamicin resistance transposon form Staphylococcus epidermidis Thomas,W.D.Jr.;G.L.Archer https://doi.org/10.1128/AAC.33.8.1335
  36. Diagnostic molecular microbiology: principles and application PCR detection of genes coding for aminoglycoside-modifying enzymes van de Klundert;J.A.M.;J.S.Vliegenthart;D.H.Persing(ed.);T.F.Smith(ed.);F.C.Tenover(ed.);T.J.White(ed.)