Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
권호 표지

Biological Evaluation of Bone Marrow-Derived Stem Cells onto Different Wettability by RT-PCR

역전사 중합효소 연쇄반응을 이용한 표면 적심성에 따른 골수유래 줄기세포의 생물학적 평가

  • 김은정 (전북대학교 유기신물질공학과) ;
  • 박종수 (한국화학연구원 나노생체의료고분자) ;
  • 김문석 (한국화학연구원 나노생체의료고분자) ;
  • 조선행 (한국화학연구원 나노생체의료고분자) ;
  • 이종문 (전북대학교 유기신물질공학) ;
  • 이해방 (한국화학연구원 나노생체의료고분자) ;
  • 강길선 (전북대학교 유기신물질공학과)
  • Published : 2004.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

The adhesion and proliferation of mammalian cells on polymeric biomaterials depend on the surface characteristics such as wettability, chemistry, charge and roughness. In order to recognize the correlation between the adhesion and proliferation of human bone marrow derived stem cells (BMSCs) and surface property, radio frequency generated plasma treatment on low density polyethylene (LDPE) has been carried out. The modified LDPE surfaces were characterized by measuring the static water contact angle. The adhesion and proliferation of cells on LDPE films were characterized by cell counting and reverse transcription-polymerase chain reaction (RT-PCR). The water contact angle of the film surface decreased with plasma treatment time. Proto-oncogenes (c-myc, c-fos) and tumor suppressor gene (p153) showed maximum expression with contact angle of 60 ∼ 70$^{\circ}$ range of LDPE film. By cell counting, we confirmed that the rate of cell proliferation appeared the higher on the film surface of the contact angle of 60∼70$^{\circ}$ We concluded that the surface wettability is an important role for the growth and differentiation of BMSCs.

고분자 생체재료에서 세포부착과 성장은 재료의 적심성, 화학구조, 표면전하 및 거칠기 등의 표면 성질에 의존한다. 본 연구에서는 저밀도 폴리에틸렌 필름 (LDPE)의 표면 적심성과 골수유래 줄기세포의 증식 및 성장성을 측정하기 위하여 플라즈마 처리를 실시하였으며 개질된 필름 표면의 특성을 조사하였다. 또한 LDPE 필름에서의 세포부착과 증식률은 세포수 관찰과 역전사 중합효소 연쇄반응으로 확인하였다. 표면성질의 하나인 물 접촉각 측정 결과 플라즈마 처리 시간이 길어짐에 따라 필름표면의 접촉각이 감소하였으며 암형성 유전자와 암억제 유전자의 발현률이 60∼70$^{\circ}$ 사이에서 높음을 확인할 수 있었다. 또한 세포수 관찰을 통해 접촉각이 60∼70$^{\circ}$인 표면에서 세포 증식률이 우수하여 표면성질이 세포의 성장과 분화에 중요함을 확인하였다.

Keywords

References

  1. Methods of Tissue Engineering G.Khang;H.B.Lee;A.Atala(Ed.);R.Lanza(Ed.)
  2. Science and Technology of Polymers and Advanced Materials, In Emerging Technologies and Business Oppeortunities J.H.Lee;J.W.Lee;G.Khang;H.B.Lee;P.N.Prasad(Ed.);J.E.Mark(Ed.);S.H.Kandil(Ed.);Z.H.Kafafi(Ed.)
  3. Advances in Biomaterials Science I Y.Iwasaki;K.Ishihara;N.Nakabayashi;G.Khang;J.H.Jeon;J.W.Lee;H.B.Lee;T.Akaike(Ed.);T.Okano(Ed.);T.Okano(Ed.);M.Akashi(Ed.);M.Terano(Ed.);N.Yui(Ed.)
  4. Cancer v.67 G.E.Moore https://doi.org/10.1002/1097-0142(19910601)67:11<2731::AID-CNCR2820671102>3.0.CO;2-1
  5. J. Biomed. Mater. Res. v.28 T.Nakamura;Y.Shimizu;N.Okumura;T.Matsui;S.H.Hyon;T.Shimamoto https://doi.org/10.1002/jbm.820280104
  6. J. Biomed. Mater. Res. v.26 A.Nakamura;Y.Kawasaki;K.Takada;Y.Aida;Y.Kurokama;S.Kojima;H.Shintani;M.Matsui;T.Nohmi;A.Matsuo;T.Sofuni;M.Kurihara;N.Miyata https://doi.org/10.1002/jbm.820260506
  7. J. Biomed. Mater. Res. v.42 T.Nakamura;Y.Shimizu;Y.Takimoto;T.Tsuda;Y.H.Li;T.Kiyotani;M.Teramachi;S.H.Hyon;Y.Ikada;K.Nishiya https://doi.org/10.1002/(SICI)1097-4636(19981215)42:4<475::AID-JBM1>3.0.CO;2-B
  8. J. Colloid Interface Sci. v.152 J.H.Lee;H.Kim;G.Khang;H.B.Lee;M.S.Jhon
  9. Bio-Med. Mater. Eng. v.5 G.Khang;B.J.Jeong;H.B.Lee;J.B.Park
  10. Bio-Med. Mater. Eng. v.6 G.Khang;Y.H.Kang;H.B.Lee;J.B.Park
  11. Biomaterials v.18 J.H.Lee;J.W.Lee;G.Khang;H.B.Lee https://doi.org/10.1016/S0142-9612(96)00128-7
  12. Macromol. Chem., Macromol. Symp. v.118 J.H.Lee;G.Khang;J.W.Lee;H.B.Lee
  13. J. Colloid Interface Sci. v.205 J.H.Lee;G.Khang;J.W.Lee;H.B.Lee https://doi.org/10.1006/jcis.1998.5688
  14. J. Biomater. Sci. Polym. Ed. v.9 Y.Iwasaki;K.Ishihara;N.Nakabayashi;G.Khang;J.H.Jeon;J.W.Lee;H.B.Lee https://doi.org/10.1163/156856298X00163
  15. J. Biomater. Sci. Polym. Ed. v.10 J.H.Lee;S.J.Lee;G.Khang;H.B.Lee https://doi.org/10.1163/156856299X00351
  16. Polymer v.7 G.Khang;S.J.Lee;J.H.Lee;H.B.Lee
  17. Biomaterials v.20 Y. Iwasaki;S.Sawada;N.Nakabayashi;G.Khang;H.B.Lee;K.Ishihara https://doi.org/10.1016/S0142-9612(99)00123-4
  18. Polymer v.8 G.Khang;S.J.Lee;Y.M.Lee;J.H.Lee;H.B.Lee
  19. J. Colloid Interface Sci. v.230 J.H.Lee;S.J.Lee;G.Khang;H.B.Lee https://doi.org/10.1006/jcis.2000.7080
  20. Polymer v.24 G.Khang;S.J.Lee;J.H.Jeon;J.H.Lee;H.B.Lee
  21. Polymer v.24 S.J.Lee;G.Khang;J.H.Lee;Y.M.Lee;H.B.Lee https://doi.org/10.1016/0032-3861(83)90207-0
  22. Korea Polymer J. v.9 G.Khang;M.K.Choi;J.M.Rhee;S.J.Lee;H.B.Lee;Y.Iwasaki;N.Nakabayashi;K.Ishihara
  23. J. Biomater. Sci. Polym. Ed. v.13 S.J.Lee;G.Khang;Y.M.Lee;H.B.Lee https://doi.org/10.1163/156856202317414375
  24. J. Appl. Polymer Sci. v.85 G.Khang;C.W.Choee;J.M.Rhee;H.B.Lee https://doi.org/10.1002/app.10680
  25. Biomaterials v.23 Y.Iwasaki;S.Sawada;N.Nakabayashi;G.Khang;H.B.Lee;K.Ishihara
  26. J. Colloid Interface Sci. v.259 S.J.Lee;G.Khang;Y.M.Lee;H.B.Lee https://doi.org/10.1016/S0021-9797(02)00163-7
  27. J. Appl. Polymer Sci. v.92 G.Khang;C.W.Choee;S.J.Lee;J.M.Rhee;H.B.Lee https://doi.org/10.1002/app.20048
  28. Biomaterials v.21 S.Kato;T.Akagi;K.Sugimura;A.Kishida;M.Akashi https://doi.org/10.1016/S0142-9612(99)00214-8
  29. Tissue Engineering : Concepts and Application (2nd Eds) H.B.Lee;G.Khang;S.J.Lee;J.J.Yoo(Ed.);I.Lee(Ed)
  30. Tissue Eng. v.4 A.M.Mackay;S.C.Beck;M.F.Pittenger;C.O.Chichester https://doi.org/10.1089/ten.1998.4.415
  31. J. Bone Joint Surg. v.80-A B.U.Yoo;T.S.Barthel;K.Nishimura;L.Solchaga;A.I.Caplan;B.Johnstone
  32. J. Cell Physiol. v.160 Y.Luo;M.O.Krause https://doi.org/10.1002/jcp.1041600212
  33. Endocrinology v.126 B.C.Delidow;B.A.White;J.J.Peluso https://doi.org/10.1210/endo-126-5-2302
  34. Cancer Res. v.54 M.S.Greenblatt;W.P.Bennett;M.Hollstein;C.C.Harris
  35. Cell v.88 A.J.Levine https://doi.org/10.1016/S0092-8674(00)81871-1
  36. Biomaterials Res. v.6 J.S.Choi;S.J.Lee;G.Khang;I.Lee;H.B.Lee
  37. Biomaterials Res. v.7 K.S.Park;E.J.Kim;G.Khang;C.W.Han;I.Lee;H.B.Lee
  38. Biomaterials Sci. D.Ratner;L.Schoen;D.Ratner(Ed.);L.Schoen(Ed.)
  39. Biochem. Biophys. Res. Commun. v.201 T.Sugimoto;F.Tsukamato;M.Fujita;S.Takai https://doi.org/10.1006/bbrc.1994.1740
  40. Cancer Lett. v.66 Y.Kaneko;A.Tsukamoto;K.Korokawa https://doi.org/10.1016/0304-3835(92)90228-N
  41. Melanoma Res. v.2 K.Sugimura;T.Ohni;Kusuyama;I.Azuma https://doi.org/10.1097/00008390-199209000-00007