Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Identification of Asian Isolates of Medicinal Mushroom Hericium erinaceum by Phylogenetic Analysis of Nuclear ITS rDNA

  • Park, Hyuk-Gu (School of Life Sciences and Biotechnology, Korea University) ;
  • Ko, Han-Gyu (School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Seong-Hwan (Department of Microbiology and Institute of Basic Science, Dankook University) ;
  • Park, Won-Mok (School of Life Sciences and Biotechnology, Korea University)
  • Published : 2004.08.01
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Abstract

A reliable molecular phylogenetic method to identify Hericium erinaceum, the most industrially valuable species in the Hericium genus, was established. Sequencing and phylogenetic analyses of the PCR-amplified ITS and 5.8S rDNA from Hericium fungi, including 6 species and 23 isolates, showed that variation in nucleotide sequences and size exists in both ITS1 and ITS2 regions, but not in the 5.8S region. These two ITS regions provided different levels of information on the relationship of H. erinaceum to other Hericium species. Based on the ITS1 sequence, both the parsimony and neighbor joining trees clearly distinguished Asian H. erinaceum isolates from other Hericium species and isolates. The intraspecific divergence of the ITS2 region was suitable to dissect the Asian H. erinaceum isolates into a few groups.

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References

  1. Mushrooms Demystified Arora, D.
  2. Trends Genet v.19 ITS2 is a double-edged tool for eukaryotic evolutionary comparisons Coleman, A. W. https://doi.org/10.1016/S0168-9525(03)00118-5
  3. Mycotaxon v.18 Hericium coralloides and Hericium alpestre basidiomycetes Hallenberg, N.
  4. J. Microbiol. Biotechnol. v.13 Isolation and characteristics of Tichoderma harzianum FJ1 producing cellulases and xylanase Kim, K. C.;S.-S.Yoo;Y.-A. Oh;S. J. Kim
  5. Appl. Environ. Microbiol. v.95 Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR Kim, S. H.;A. Uzunovic;C. Breuil
  6. Mycol. Res. v.105 Phylogeographic divergences of nuclear ITS sequences in Coprinus species sensu lato Ko, K. S.;Y. M. Lim;Y. H. Kim;H. S. Jung https://doi.org/10.1017/S0953756201005184
  7. J. Microbiol. Biotechnol. v.12 Development of rapid molecular detection marker for Colletrotrichum spp. in leaf and fruit tissues of sweet persimmon Lee, S.-P.;Y.-S.Lee
  8. Edible Fungi of China v.18 Survey in medicinal value of Hericium erinaceus Liu, M.;H. Cheng;H. Sun
  9. Biol. Pharm. Bull. v.25 PCR-based sensitive detection of medicinal fungi Hericium species from ribosomal internal transcribed spacer (ITS) sequences Lu, L.;J. Li;Y. Cang https://doi.org/10.1248/bpb.25.975
  10. Int. J. Med. Mush v.1 Bioactive substances in Hericium erinaceus (Bull.; fr.) Per. (Yamabushitake), and its medicinal utilization Mizuno, D. https://doi.org/10.1615/IntJMedMushrooms.v1.i2.10
  11. Biosci. Biotech. Biochem. v.56 Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum, and edible and medicinal mushroom called yamabushitake or houtou Mizuno, T.;T. Wasa;H. Ito;C. Suzuki;N. Ukai https://doi.org/10.1271/bbb.56.347
  12. Mycologist v.9 Sequence or structure? A short review on the application of nucleic acid sequence information to fungal taxonomy Mitchell, J. I.;P. J. Roberts;S. T. Moss https://doi.org/10.1016/S0269-915X(09)80212-7
  13. J. Microbiol. Biotechnol. v.12 Secondary structure and phylogenetic implications of ITS2 in the genus Tricholoma Suh, S. J.;J. G. Kim
  14. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods) Swofford, D. L.
  15. Clin. Microbiol. Rev. v.12 The evolutionary biology and population genetics underlying fungal strain typing Taylor, J. W.;D. M. Geiser;A. Burt;V. Koufopanou
  16. Nucleic Acids Res. v.22 CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice Thompson, J. D.;D. G. Higgins;T. J. Gibson https://doi.org/10.1093/nar/22.22.4673
  17. Kaohsiung J. Med. Sci. v.17 Antitumor and immunoenhancing activities of polysaccharide from broth of Hericium spp. Wang, J.-C.;S.-H. Hu;C.-H. Su;T.-M. Lee
  18. PCR Protocols: A Guide to Methods and Applications Ampliication and diect sequencing of fungal ribosomal RNA genes for phylogenetics White, T. J.;T. Bruns;S. Lee;J. Taylor;M. A. Innis(ed.);D. H. Gelfand(ed.);J. J. Sninsky(ed.);T. J. White(ed.)
  19. J. Microbiol. Biotechnol. v.12 Hypolipideic effect of exo-polymer produced in submerged mycelial culture of five diferent mushrooms Yang, B. K.;J. B. Park;C. H. Song