Functional Complementation of Escherichia coli by the rpoS Gene of the Foodborne Pathogenic Vibrio vulnificus

  • Park, Kyung-Je (Department of Environmental Science, Hankuk University of Foreign Studies) ;
  • Kim, Song-Hee (Department of Environmental Science, Hankuk University of Foreign Studies) ;
  • Kim, Min-Gon (Laboratory of Intergrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University) ;
  • Jahng, Deok-jin (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Lee, Kyu-Ho (Department of Environmental Science, Hankuk University of Foreign Studies)
  • Published : 2004.10.01

Abstract

The rpoS gene product is a global transcriptional factor, which is involved in bacterial survival under various stress conditions. An rpoS-homologous gene was cloned from a septicemia-causing pathogenic Vibrio vulnificus. Introduction of this gene as a multicopy plasmid into various E. coli strains displayed functional complementation, for examples, increased survivability of an rpoS-defective E. coli cell and induction of known $\delta^S$-dependent, stress-responding promoters of E. coli genes.

Keywords

References

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