Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibitory Effect of Methanol Extracts and Solvent Fractions from Doenjang on Mutagenicity Using in vitro SOS Chromotest and in vivo Drosophila Mutagenic System

된장 메탄올 추출물 및 분획물에 의한 in vitro SOS Chromotest 실험계와 in vivo 초파리 돌연변이 검출계에서의 항돌연변이 효과

  • Lim, Sun-Young (Division of Ocean and Science, Korea Maritime University) ;
  • Lee, Sook-Hee (Dept. of Food Science and Nutrition, Busan National University) ;
  • Park, Keun-Young (Dept. of Food Science and Nutrition, Busan National University) ;
  • Yun, Hee-Sun (Dept. of Biology, Busan National University) ;
  • Lee, Won-Ho (Dept. of Biology, Busan National University)
  • Published : 2004.11.01
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Abstract

This study investigated the inhibitory effect of methanol extracts and several solvent fractions from doen-jang on mutagenicity using in vitro SOS chromotest and in vivo Drosophila mutagenic system. In order to determine an antimutagenic effect of doenjang methanol extracts, other soybean fermented foods and original materials were compared. The treatment of doenjang methanol extracts (100 ${\mu}$/assay) to SOS chromotest system inhibited N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced mutagenicity by 87~97% and showed higher antimutagenic effect than other fermented foods. Among solvent fractions from doenjang methanol extracts, the ethylacetate and dichloromethane fractions showed the stronger antimutagenic effect (91% and 95%, respectively) in SOS chromotest. In Drosophila mutagenic system, the treatment of ethylacetate fraction (5%/bottle) significantly inhibited aflatoxin $B_1$ induced mutagenicity by 97%. These results demonstrated that doenjang had an inhibitory effect to mutagenic agents in both in vitro and in vivo mutagenic systems, suggesting that its antimutagenic effect may be due to active compounds in the ethylacetate fraction from doenjang methanol extracts.

In vitro SOS chromotest 실험계에서 콩된장 메탄올 추출물의 경우 100 ${\mu}$g/assay 첨가시 97%의 돌연변이 억제효과를 70% 콩된장 메탄올 추출물은 87%의 억제효과를 가졌고, 청국장, 미소, 원재료인 콩과 콩/밀가루 메탄올 추출물보다 높은 저해 효과를 보였다. 된장의 메탄올 추출물을 더욱 분획하여 얻어진 분획물들, 즉 헥산, 메탄올, 디클로로메탄, 에틸아세테이트, 부탄올, 물 분획물을 100 ${\mu}$g/assay 첨가했을 때 MNNG에 대한 돌연변이 억제효과는 각각 73%, 73%, 91%, 95%, 82%, 73%로 전반적으로 매우 높은 저해효과를 나타내었으며, 특히 디클로로메탄과 에틸아세테이트 분획물의 항돌연변이 효과가 가장 높았음을 관찰할 수가 있었다. 초파리의 wing hairs spots 시스템의 small mwh spots의 경우, $AFB_1$ 단독 처리군을 체세포 돌연변이 유발 100%로 기준해서 된장 메탄올 추출물 2.5%, 5% 첨가농도의 경우, 각각 22%, 30%의 낮은 항돌연변이 효과를 나타내었다. 분획물들 중 가장 활성이 높았던 된장의 에틸아세테이트 분획물 5%, 10% 첨가농도의 경우, 각각 97%의 높은 항돌연변이 효과를 가짐으로 낮은 농도에서부터 강하게 체세포 돌연변이를 억제함을 알 수 있었고, large mwh spots의 경우는 된장의 에틸 아세테이트 분획물 5%의 낮은 농도에서 체세포 염색체 재조환이나 mwh+ 좌위에 유전자 돌연변이를 현저하게 억제하는 것으로 나타났다. 이상의 결과로부터 된장 메탄올 추출물은 다른 콩 관련 발효식품과 콩의 메탄올 추출물에 비해 in vitro SOS chromotest에서 높은 돌연변이 유발 억제 작용을 나타냈고 분획물들 중 에틸아세테이트 분획물은 in vitro와 in vivo 돌연변이 유발실험에서 가장 높은 저해효과를 나타내어 된장의 항돌연변이 활성을 가지고 있는 분획물로 추정되어진다.

Keywords

References

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