Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effects of the $MnCl_2$ on bone formation in fetal rat calvarial cell

망간이 백서 두개관세포의 골형성능에 미치는 영향

  • Han, Woong-Taek (Dept. of Periodontology, Chonnam National University) ;
  • Ciu, De-Zhe (Graduate school Dept. of Dental science, Chonnam National University) ;
  • Kim, Young-Joon (Dept. of Periodontology, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Chung, Hyun-Ju (Dept. of Periodontology, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Ok-Su (Dept. of Periodontology, Chonnam National University, Dental Science Research Institute, Chonnam National University)
  • 한웅택 (전남대학교 치과대학 치주과학교실) ;
  • 최득철 (전남대학교 대학원 치의학과) ;
  • 김영준 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 정현주 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 김옥수 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소)
  • Published : 2004.12.31
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Abstract

Chronic exposure to high levels of manganese (Mn) leads a pronounced and debilitating disorder known as manganism. Research on the toxic manifestation of manganese have focused primarily on its neurological effects because exposure to high levels of the metal produces a distinct and irreversible extrapyramidal dysfunction resembling the dystonic movements associated with Parkinson's physiological and biochemical systems in the body. The purpose of this study is to determine the effects of Mn on mineralization in primary rat calvarial cells. The experimental groups were in concentration of 0, 10, 30 and 60 ${\mu}M$. The results were as follows: 1. ALP activity was decreased in concentration of 30 and 60 ${\mu}M$ (p<0.01). 2. Bone nodule formation was depressed in concentration of 30 and 60 ${\mu}M$ at day 14 and 21 (p<0.01). 3. RT-PCR results showed an altered expression of bone matrix proteins. These result suggested that manganese might decrease or alter the expression of the osteoblast phenotype.

Keywords

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