온실가루이와 담배가루이에 대한 Spearmint Oil의 살충활성

Insecticidal Activity of Spearmint Oil against Trialeurodes vaporariorum and Bemisia tabaci Adults

  • 최유미 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Choi Yu-Mi (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim Gil-Hah (Dept. of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 발행 : 2004.12.01

초록

본 연구는 시설하우스의 주요 해충인 온실가루이와 담배가루이에 대한 spearmint oil의 훈증독성 및 접촉독성을 조사하였으며, gas chromatograph (GC)와 gas chromatograph mass spectrometry(GC/MS)를 이용하여 speamint oil의 주요 구성성분을 분석하였다. 훈증독성 시험 결과, 온실가루이에 대해서 spearmint oil은 $10{\mu}L/954mL$ air의 약량처리에서 $99.1\%$의 살충활성을 나타내었으며, 5와 $1{\mu}L/954mL$ air의 약량처리에서 각각 $91.7\%$$41.1\%$의 살충활성을 나타내었다. 또한 담배가루이에 대해서 spearmint oil은 $10{\mu}L/954mL$ air의 약량처리에서 각각 $100\%$의 살충활성을 나타내었으며, 5와 $1{\mu}L/954mL$ air의 약량에서도 각각 100, $61.3\%$의 높은 살충활성을 나타내었다. 그러나 온실가루이와 담배가루이 성충에 대한 spearmint oil의 접촉독성은 살충효과가 $30\%$ 이하로 낮았다. Spearmint oil을 GC와 GC/MS로 분석한 결과, limonene ($16.1\%$), ${\gamma}$-terpinene($13.8\%$), ${\rho}$-cymene($5.8\%$), 3-octanol($6.9\%$), carvone($40.9\%$)이 주요 구성성분으로 나타났으며, 주요성분인 carvone 은 $10{\mu}L/954mL$ air 약량에서 $100\%$의 높은 훈증독성을 나타내었다.

These studies were carried out to investigate the fumigation and contact toxicities of spearmint oil (Mentha spicata) against adults of greenhouse whitefly, Trialeurodes vaporariorum and sweet-potato whitefly, Bemisia tabaci. And we carried out the constituent analysis of spearmint oil using gas chromatograph (GC) and gas chromatograph mass spectrometry (GC/MS). Spearmint oil showed $99.1\%,\;91.7\%,\;41.1\%$ fumigation toxicity against T. vaporariorum adults at $10{\mu}L/954mL,\;5{\mu}L/954mL,\;1{\mu}L/954mL$ air concentration, respectively. In case of B. tabaci adults, spearmint oil showed $100\%,\;100\%,\;61.3\%$ fumigation toxicity, respectively. However, spearmint oil showed < $30\%$ contact toxicity against adults of T. vaporariorum and B. tabaci. Through the constituent analysis using GC and GC/MS, we confirmed main constituents of spearmint oil were limonene ($16.1\%$), ${\gamma}$-terpinene($13.8\%$), ${\rho}$-cymene($5.8\%$), 3-octanol($6.9\%$), carvone($40.9\%$). Carvone, major constituent of spearmint oil, also showed $100\%$ fumigation toxicity at $10{\mu}L/954mL$ air concentration.

키워드

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