온도조건에 따른 파밤나방핵다각체병바이러스(SeNPV)의 병원 활성

Effects of Different Temperatures on Pathogenicity of Spodoptera exigua Nucleopolyhedrovirus (SeNPV)

  • 김선곤 (전남농업기술원 식물환경연구과) ;
  • 박종대 (전남농업기술원 식물환경연구과) ;
  • 김도익 (전남농업기술원 식물환경연구과) ;
  • 최형국 (전남농업기술원 식물환경연구과) ;
  • 김상수 (순천대학교 응용생물학과) ;
  • 황인천 ((주)경농중앙연구소)
  • Kim Seon-Gon (Div. of Plant Environ. Res., Jeonnam Agricultural Research and Extention Services) ;
  • Park Jong-Dae (Div. of Plant Environ. Res., Jeonnam Agricultural Research and Extention Services) ;
  • Kim Do-Ik (Div. of Plant Environ. Res., Jeonnam Agricultural Research and Extention Services) ;
  • Choi Hyeong-Gug (Div. of Plant Environ. Res., Jeonnam Agricultural Research and Extention Services) ;
  • Kim Sang-Soo (Dep. of Applied Biology, Sunchon National University) ;
  • Hwang In-Cheon (Central Research institute Kyungnong Co.)
  • 발행 : 2004.12.01

초록

파밤나방 핵다각체병바이러스(Spodoptera exigua nucleopolyhedrovirus: SeNPV)의 온도별, 농도별로 병원활성을 조사한 결과는 다음과 같다. SeNPV의 온도에 따른 $LC_{50}$은 2령 $20^{\circ}C$에서는 $9.797{\times}10^{3}PIBs/mL$, $32^{\circ}C$에서는 $3.351{\times}10^{2}PIBs/mL$로 온도가 높아질수록 낮아졌으며, 다른 영기에서도 같은 경향이었다. $20^{\circ}C$에서 SeNPV $1.0{\times}10^{3}PIBs/mL$의 파밤나방 3령 유충에 대한 $LT_{50}$은 9.0일이었으나 SeNPV $1.0{\times}10^{4-6}PIBs/mL$에서는 6.9일-3.5일로 더 짧았다. 24, 28, $32^{\circ}C$의 SeNPV $1.0{\times}10^{4}PIBs/mL$에서는 5.7일, 5.5일, 4.9일의 병원 활성을 보여 온도가 올라갈수록 $LT_{50}$은 짧아졌다. 또한 바이러스 농도가 높고 유충의 영기가 어릴수록 짧아져 $LT_{50}$은 온도 바이러스 농도, 유충영기에 따라 차이가 있었다.

This experiment was conducted to investigate pathogenicity of Spodoptera exigua nucleopolyhedrovirus (SeNPV) with different temperatures for mass production. In laboratory condition, $LC_{50}$ values of SeNPV were $9.797{\times}10^{3}PIBs/mL$ at $20^{\circ}C$ and $3.351{\times}10^{2}PIBs/mL$ at $32^{\circ}C$ in 2nd instar larvae. $LC_{50}$ of the other larval stage were similar to that of 2nd instar. $LT_{50}$ values of SeNPV was 9.0 days in $1.0{\times}10^{3}PIBs/mL$ but 6.9 to 3.5 days in $1.0{\times}10^{4-6}PIBs/mL$ against 3rd instar of Spodoptera exigua. $LT_{50}$ Values of $1.0{\times}10^{4}PIBs/mL$ were 5.7, 5.5 and 4.9 days in 24, 28 and $32^{\circ}C$, respectively. As a results, $LT_{50}$ was shortened with increase of temperatures up to $32^{\circ}C$ and also dependent on viral concentration and larval instars.

키워드

참고문헌

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