Membrane Journal (멤브레인)

The Membrane Society of Korea (한국막학회)
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Separation and Purification of Lysozyme from Chicken Eggwhite Through Ultrafiltration

한외여과를 통한 난백 중 라이소자임의 분리정제

  • Koo Ja-Kyung (Department of Applied Chemical Engineering, Korea University of Technology and Education) ;
  • Son Dongho (Department of Applied Chemical Engineering, Korea University of Technology and Education) ;
  • Jun Hoejin (Department of Applied Chemical Engineering, Korea University of Technology and Education) ;
  • Lee Yunhee (Department of Applied Chemical Engineering, Korea University of Technology and Education) ;
  • Cho Namjun (Department of Applied Chemical Engineering, Korea University of Technology and Education) ;
  • Jang Dong Il (Cotde Co. Inc.)
  • 구자경 (한국기술교육대학교 응용화학공학과) ;
  • 손동호 (한국기술교육대학교 응용화학공학과) ;
  • 전회진 (한국기술교육대학교 응용화학공학과) ;
  • 이윤희 (한국기술교육대학교 응용화학공학과) ;
  • 조남준 (한국기술교육대학교 응용화학공학과) ;
  • 장동일 ((주)콧데)
  • Published : 2005.06.01
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Abstract

Separation and purification of lysozyme from chicken egg white was studied using ultrafiltration. We have obtained experimental data through the cellulose membranes with the molecular weight cut off (MWCO) of 10 kDa, 30 kDa and 100 kDa in a stirred ultrafiltration device. Certain amounts of egg white were dissolved into 20 mM phosphate buffers of pH 6, 7 and 8 to make protein solutions of $1\%,\;2\%,\;3\%\;and\;10\%$ concentration. Permeation flux increased with increasing MWCO of the membrane. Permeation flux increased with increasing transmembrane pressure (TMP) and decreasing the protein concentration. As the MWCO of membrane decreased, the selectivity increased. The selectivity increased with increasing TMP and protein concentration of the solution.

한외여과를 통하여 난백으로부터 라이소자임을 분리, 정제하는 연구를 수행하였다. 교반식 한외여과장치에 분획 분자량(MWCO)이 각각 10 kDa, 30 kDa 및 100 kDa의 셀룰로즈 재질 분리막을 사용하여 알부민과 라이소자임의 투과실험 데이터를 수집하였다. 단백질 용액은 20 mM, pH 6, 7, 8의 인산염 완충용액에 난단백 함량을 $1\%,\;2\%,\;3\%$ 및 $10\%$ 첨가하여 제조하였으며, 막간압력차(TMP)는 $0.5\~3$ bar의 구간에서 실험을 하였다. 분리막의 분획분자량이 증가하면서 투과유속은 증가하였으며, 분리막에서의 막간압력차가 증가하면서 그리고 공급원액의 난백농도가 감소하면서 투과유속은 증가하였다. 또한, 분리막의 분획분자량이 감소하면서 알부민의 라이소자임에 대한 선택도는 증가하였으며, 모든 분리막에서 pH 와 공급원액의 난백농도 그리고 막간압력차가 증가하면서 라이소자임의 알부민에 대한 선택도는 증가하였다.

Keywords

References

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