동백나무(Camellia japonica L.) 잎 추출물이 식품유해 미생물에 미치는 항균 효과

Antimicrobial Effects of Camellia Japonica L. Leaves Extract on Food-borne Pathogenic Microorganisms

  • 한영숙 (성신여자대학교 식품영양학과)
  • 발행 : 2005.02.28

초록

인체에 무해한 천연 항균 소재를 탐색하기 위한 목적으로 국내에 자생하는 동백나무(Camellia japonica L.) 잎의 methanol 추출물 및 분획물로 4종의 식품 유해 미생물(Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes)에 대한 항균활성을 살펴보았다. Paper disc 법을 사용하여 조사한 용매별 항균효과는 methanol 추출물이 4종의 시험균주 모두에서 항균효과를 나타내었다. 특히 S. aureus와 L. monocytogenes에 대하여 강한 항균효과를 나타내었다. 분획물 중에서는 water 분획물과 butanol 분획물이 S. aureus에 대하여 강한 항균효과를 나타내었다. n-hexane 분획물과 chloroform 분획물은 4종의 시험균주 모두에서 항균효과를 나타내지 않았으며, ethylacetate 분획물은 S. aureus에 대해서만 항균효과를 나타내었다. 동백나무 잎 methanol 추출물과 용매별 분획물을 농도별로 처리하여 균들의 생육도를 균주별로 조사한 결과, 공통 적으로 모든 균들이 methanol 추출물에서 항균효과를 나타내었고, 분획물 중에서는 butanol 분획물, water 분획물에서 항균효과가 뚜렷이 나타났다. 항균 저해율을 확인한 결과, methanol 추출물은 모든 균에 대해 약 25% 이상의 저해율을 나타내었다. 분획물 중에서는 butanol 분획물이 L. monocytogenes를 제외한 균에서 50% 이상의 강한 저해율을 나타내었으며 ethylacetate 분획물과 water 분획물에서는 각각 S. aureus, L. monocytogenes가 30% 이상의 높은 생육 저해율을 나타내었다. 균의 최소저해 농도(MIC)를 측정한 결과는 S. typhimurium에 대하여 methanol 추출물이 $625{\mu}g/mL$의 농도를 나타내었다. 분획물 중에서 S. aureus에 대하여 butanol 분획물이 $625{\mu}g/mL$의 농도로 가장 낮은 값을 나타내어 적은 양으로도 항균활성을 나타낼 수 있음을 알 수 있었다.

Antimicrobial effects of Camellia japonica L. were determined against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Listeria monocytogenes using paper disc method, and minimum inhibitory concentrations (MICs) were measured. Methanol extract (MEex), water fraction (WAfr), and butanol fraction(BUfr) showed antimicrobial effects against all tested microorganisms, with MEex showing strong antimicrobial effect against S. aureus and L. monocytogenes, and WAfr, Bufr, and ethylacetate fraction (EAfr)against S. aureus. No effects were observed in n-hexane fraction (HEfr) and chloroform fraction (CHfr) against all tested microorganisms. All species grown in the medium adding fractions of Camellia Japonica L. leaves extact were inhibited from WAfr and BUfr, repectively.(meaning not clear) MEex showed over 25% inhibitory effect against all tested microorganisms. BUfr showed over 50% inhibitory effect against all microorganisms except L. monocytogenes. EAfr and WAfr showed over 30% effect against S. aureus and L. monocytogenes. MICs of MEex against S. typhimurium and BUfr against S. aureus were 625 g/mL, indicating C. japonica L. extract can exert antimicrobial activity even at low concentration.

키워드

참고문헌

  1. Oh DH, Ham SS, Park BK, Ahn C, Yu JY. Antimicrobial Activities of natural medicinal herbs on the food spoilage or foodborne disease microorganisms. Korean J. Food Sci. Technol. 30: 957-963 (1998)
  2. Ahn YS, Shin DH, Baek NI. Isolation and identification of active antimicrobial substance against Listeria monocytogenes from Ruta graveolens Linne. Korean J. Food Sci. Technol. 32: 1379-1388 (2000)
  3. Park HO, Kim CM, Woo GJ, Park SH, Lee DH, Chang EJ, Park KH. Monitoring and trends analysis of food poisoning outbreaks occurred in recent years in Korea. J. Food Hyg. Safety, 16: 280-294 (2001)
  4. Beuchat LR. Survival of enterohemorrhagic Escherichia coli O157:H7 in bovine feces applied to lettuce and effectiveness of chlorinated water as disinfectant. J. Food Prot. 62: 845-849 (1999)
  5. Castillo A, Lucia LM, Kemp GK, Acuff GR. Reduction of Escherichia coli O157:H7 and Salmonella typhimurium on beef carcass surfaces using acidified sodium chloride. J. Food Prot. 62: 580-584 (1999)
  6. Venkitanarayanan KS, Ezeike GOI, Hung YC, Doyle MP. Inactivation of Escherichia coli O157:H7 and and Listeria monocytogenes on plastic kitchen cutting boards by electrolyzed oxidizing water. J. Food Prot. 62: 857-860 (1999)
  7. Sapers GM, Miler RL, Mattazzo AM. Effectiveness of sanitizing agents in inactivating Escherichia coli in golden delicious apples. J. Food Sci. 62: 734-737 (1999)
  8. Taormina PJ, Beuchat LR. Behavior of enterohemorrhagic Escherichia coli O157:H7 on alfalfa sprouts during the sprouting process as influenced by treatments with various chemicals. J. Food Prot. 62: 850-856 (1999)
  9. Kang DH, Fung DYC. Effect of diacetyl controlling Escherichia coli O157:H7 and Salmonella typhimurium in the presence of starter culture in a laboratory medium and during meat fermentation. J. Food Prot. 62: 975-979 (1999)
  10. Larocco KA, Martin SE. Effects of potassium sorbate alone and in combination with sodium chloride on the growth of Salmonella typhimurium 7136. J. Food Sci. 46: 568-570 (1981) https://doi.org/10.1111/j.1365-2621.1981.tb04912.x
  11. Yousef AE, EL-Shenawy MA, Marth EH. Inactivation and injury of Listeria monocytogenes in a minimal medium as affected by benzoic acid and incubation temperature. J. Food Sci. 54: 650-652 (1989) https://doi.org/10.1111/j.1365-2621.1989.tb04673.x
  12. Juven BJ, Barefoot SF, Pierson MD, Maccaskill LH, Smith B. Growth and survival of Listeria monocytogenes in vacuum-packaged ground beef inoculated with Lactobacillus alimentarius Flora Cam L-2. J. Food Prot. 61: 551-556 (1998)
  13. Schobitz R, Zaror T, Leon O, Costa M. A bacteriocin from Carnobacterium piscicola for the control of Listeria monocytogenes in vacuum packaged meat. Food Microbiol. 16: 249-255 (1998) https://doi.org/10.1006/fmic.1998.0241
  14. Smith JL, Marmer BS. Growth temperature and action of lysozyme on Listeria monocytogenes. J. Food Sci. 56: 1101-1103 (1991) https://doi.org/10.1111/j.1365-2621.1991.tb14651.x
  15. Stecchini ML, Luch RD, Bortolussi G, Deltorre M. Evaluation of lactic acid and monolaurin to control Listeria monocytogenes on stacchino cheese. Food Microbiol. 13: 483-488 (1996) https://doi.org/10.1006/fmic.1996.0055
  16. Sheo HJ. The Antibacterial action of garlic, onion, ginger and red pepper juice. J. Korean Soc. Food Sci. Nutr. 28: 94-99 (1999)
  17. Lee SH, Lim YS. Antimicrobial effects of Schizandra chinensis extract on pathogenic microorganism. J. Korean Soc. Food Sci. Nutr. 27: 239-243 (1998)
  18. Jean YO, Kim SI, Han YS, Kim KH. Screening of antimicrobial activity of the Plantain (Plantago asiatica L.) extract. Korean J. Soc. Food Sci. 14: 498-502 (1998)
  19. Kang SK, Sung NK, Kim YD, Shin SC, Sea JS, Choi KS, Park SK. Screening of antimicrobial activity of leaf mustard (Bras sica Juncea) extract. J. Korean Soc. Food Nutr. 23: 1008-1013 (1994)
  20. Sea KL, Kim DY, Yang SI. Studies on the antimicrobial effect of wasabi extracts. Korean J. Nutr. 28: 1073-1077 (1995)
  21. Park UK, Chang DS, Cho HR. Screening of antimicrobial activity for medicinal herb extracts. J. Korean Soc. Food Nutr. 21: 91-96 (1992)
  22. Shin DH, Kim MS, Han JS. Antimicrobial effect of ethanol extracts from some medicinal herbs and their fractionates against food-born bacteria. Korean J. Food Sci. Technol. 29: 808-816 (1997)
  23. Nam SH, Yang MS. Antibacterial activities of extracts from chrysanthemun boreale M. J. Korean Soc. Appl. Bio. Chem. 38: 269-272 (1995)
  24. Yang MS, Ha YL, Nam SH, Choi SU, Jang DS. Screening of domestic plants with antibacterial activity. J. Korean Soc. Appl. Bio. Chem. 38: 584-589 (1995)
  25. Lee SH, Kim SK. Natural distribution and characteristics of populations of Camellia japonica in Korea. J. Korean Soc. Hort. Sci. 33: 196-208 (1992)
  26. Itokawa H, Nakajima H, Ikuta A, Iitaka Y. Two teiterpenes from the flowers of Camellia japonica. Phytochem. 20: 2539-2542 (1981) https://doi.org/10.1016/0031-9422(81)83089-0
  27. Yoshikawa M, Harada E, Murakami T, Matsuda H, Yamahara J, Murakami N. Camellia saponnins B1, B2, C1 and C2, new type inhibitors of ethanol absorption in rats from the seeds of Camellia japonica L, Chem. Pharm. Bull. 42: 742-749 (1994) https://doi.org/10.1248/cpb.42.742
  28. Fujita Y, Fujita H, Yoshikawa H. Comparative biochemical and chemotaxonomical studies of the plants of Theaceae (I), Essential oils of Camellia sasanqua Thunb., C. japonica Linn., and Thea sinensis Linn. Osaka kogyo Gijutsu Shikensho Kigo. 25: 198-202 (1973)
  29. Kim KY, Davidson PM, Chung HJ, Antibacterial activity in extracts of Camellia japonica L. petals and its application to a model food system, J. Food Prot. 64: 1255-1260 (2001)
  30. Lee CB. Korean plants pictorial book. Hyangmoon Publishing Co., Korea (1999)
  31. Conner DE, Beuchat LR. Effect of essential oils from plants on growth of food spoilage yeast. J. Food Sci. 49: 429-434 (1984) https://doi.org/10.1111/j.1365-2621.1984.tb12437.x
  32. Amsterdam D. Susceptibility Testing of Antimicrobials in Liquid Media, Antibiotics in Laboratory Medicine. 4th ed. Williams and Wilkins, MD, USA. pp. 52-111 (1996)
  33. Kong YJ, Park BK, Oh DH. Antimicrobial activity of quercus mongolica leaf ethanol extract and organic acids against foodborne microorganisms. Korean J. Food Sci. Technol. 33: 178-183 (2001)
  34. Tabak M, Armom R, Potasman I, Neeman I. In vitro inhibition of Helicobacter pylori by extracts of thyme. J. Appl. Bacteriol. 80: 667-672 (1996) https://doi.org/10.1111/j.1365-2672.1996.tb03272.x
  35. Lee JJ, Kim SH, Chang BS, Lee JB, Huh CS, Kim TJ, Baek YJ. The antimicrobial activity of medicinal plants extracts against Helicobacter Pylori. Korean J. Food Sci. Technol. 31: 764-770 (1999)