Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Identification of Two Psoralen Derivatives as Antioxidative Compounds from the Aerial Parts of Angelica keiskei

신선초에 함유된 항산화활성물질 Psoralen 유도체들의 단리 및 동정

  • Kim, So-Joong (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Cho, Jeong-Yong (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Wee, Ji-Hyang (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Jang, Mi-Young (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Rim, Yo-Sup (Division of Environmental and Agricultural Science, Sunchon National University) ;
  • Kim, Cheol (SHALOM Industries Co., Ltd.) ;
  • Shin, Soo-Cheol (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Moon, Jae-Hak (Division of Food Science, Sunchon National University) ;
  • Park, Keun-Hyung (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University)
  • 김소중 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 조정용 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 위지향 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 장미영 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 임요섭 (순천대학교 환경농업과학부) ;
  • 김철 (샬롬산업(주)) ;
  • 신수철 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 문제학 (순천대학교 식품과학부) ;
  • 박근형 (전남대학교 식품공학과 및 기능성식품연구센터)
  • Published : 2005.08.31
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Abstract

Hot water extracts of Angelica keiskei aerial parts were solvent-fractionated with ethyl acetate (EtOAc) and buffers (5% $NaHCO_3$, pH 8.0; 1.0 N HCl, pH 3.0) to obtain EtOAc-soluble acidic and neutral fractions. EtOAc-soluble neutral fraction was purified by Sephadex LH-20 column chromatography and reverse phase HPLC. Assay for purification of antioxidative compounds was performed by spraying DPPH solution on thin layer chromatography. Two isolated substances were identified as pseudoisopsoralen and 8-methoxypsoralen(xanthotoxin) by FAB-MS and NMR analyses.

신선초의 기능성 해명과 유용 기능성성분 탐색연구의 일환으로 신선초 지상부로부터 항산화활성물질을 분리하여 구조 해석하였다. 신선초 지상부의 열수추출물을 용매분획하여 ethyl acetate 가용 중성획분을 얻었다. 이를 Sephadex LH-20 column chromatography와 HPLC로 정제하여 항산화활성물질 2종을 단리하고, 그들 각각의 화합물을 대상으로 FAB-MS와 NMR 분석을 실시한 결과, pseudoisopsoralen과 8-methoxypsoralen(xanthotoxin)으로 동정하였다.

Keywords

References

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