Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Growth Inhibition Effects of Cancer Cell Lines by Gloiopeltis furcata Fractions in Vitro

불등가사리 분획물의 암세포 성장 억제 효과

  • Park, Soung-Young (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Jung, Bok-Mi (Dept. of Food Science and Nutrition, Yosu National University) ;
  • Choi, Yung-Hyun (Dept. of Biochemistry, College of Oriental Medicine and Research Institute of Orental Medicine, Dong-Eui University) ;
  • Bae, Song-Ja (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
  • 박성영 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 정복미 (여수대학교 식품영양학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터)
  • Published : 2005.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

The growth inhibitory effects on human cancer cell lines provide useful information regarding critical cellular targets. Reports on cytotoxicity of Gloiopeltis furcata (GF) to human cancer cell lines are conflicting. This study was performed to investigate the effects of cytotoxicity and quinone reductase activity of Gloiopeltis furcata on the human cancer cells. The four partition layers of methanol extracts (GFM) which are hexane (GFMH), methanol (GFMM), butanol (GFMB) and aquous (GFMA) were screened for their cytotoxic effects on HepG2, HeLa, MCF-7, HT-29, and normal liver cell lines. The GFMM showed the strongest growth inhibition effect on all cell lines we used. the GFMM showed the highest induction activity of quinone reductase on HepG2 cells among the other partition layers.

본 실험은 홍조해조류의 하나인 불등가사리를 추출 후 각 용매별로 분획하여 암세포 성장억제 효과와 QR유도활성 효과 등 생리활성을 연구하였다. 불등가사리를 이용하여 4종의 인체 암세포주HepG2, HeLa, MCF-7및 HT-29와 정상 간세포에 대한 암세포 증식억제 실험을 한 결과 사용한 4종의 암세포주에서 모두 시료첨가 농도에 의존적으로 증식 저지 효과가 나타났고, 특히 불등가사리의 methanol 분획층인 GFMM에서는 매우 낮은 농도의 시료첨가에도 불구하고 괄목할 만한 높은 암세포 성장 억제효과를 나타내었으며, 정상 간세포에서는 모든 분획층에서 약한 세포증식 억제율을 나타내어 정상 세포에 미치는 독성이 낮음을 확인하였다. 특히 본 시료는 여성암의 대표적인 유방암과 자궁경부암의 암세포 성장저지효과가 탁월하였다. 한편, 사용한 4가지 암세포주 중 유일하게 QR을 가지고 있는 HepG2를 이용한 암 예방지표인 QR 효소 유도 활성 여부를 측정한 결과 분획물 첨가농도를 10, 20, 30 및 40 $\mu$g/mL로 첨가하였을 때 GFMM의 첨가농도 $20\mu g/mL$에서 대조군에 비해 약 2배 이상의 높은 QR 유도효과를 나타내어 최종농도 $40\mu g/mL$에서는 2.6의 암 예방 QR 유도효과를 나타내었다.

Keywords

References

  1. Stavric B. 1994. Role of chemopreventiers in human diet. Clin Biochem 27: 319-320 https://doi.org/10.1016/0009-9120(94)00039-5
  2. Reddy L, Odhav B, Bhoola KD. 2003. Natural products for cancer prevention: a global perspective. Pharmacology and Therapeutics 99: 1-13 https://doi.org/10.1016/S0163-7258(03)00042-1
  3. 분자암학회 한국유전자 이식 연구재단. 2000. Cancer chemoprevention
  4. Doll R, Peto R. 1981. The cause of cancer. Quantitative estimates of available risks of cancer in the United States today. J Natl Cancer Inst 66: 1192-1308 https://doi.org/10.1093/jnci/66.6.1192
  5. Banner SE, Pastorino U, Lippman SM, Hong WK. 1994. Second international cancer chemoprevention conference. Cancer Res 54: 854-859
  6. Ham SS, Lee SY, Choi M, HwangBo HJ. 1998. Antimutagenicity and cytotoxicity effect of Woorimil wheat flour extracts added with wild herb and seaweed powder. J Korean Soc Food Sci Nutr 27: 1177-1182
  7. Choi HJ, Bae SJ, Kim ND, Jung JH, Choi YH. 2004. Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells. Int J Mol Med 14: 1091-1096
  8. Ha MS, Kim DS, Ryu BH, Ji BH. 1986. Desmutagenic effect of extracts obtained from seaweeds. J Kor Fish Soc 19: 502-508
  9. Ryo T, Hiroko IS, Kaeko H, Saburo H, Susumu H. 1998. Concurrence of agaroid and carrageenan chains in funoran from the red seaweed Gloiopeltis furcata post. et ruprecht. Carbohydrate Polymers 35: 81-87 https://doi.org/10.1016/S0144-8617(97)00230-0
  10. Bae SJ. 2004. Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33: 480-486 https://doi.org/10.3746/jkfn.2004.33.3.480
  11. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbrott BJ, Mayo JG, Shoemaker RH, Boyd MR. 1988. Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601
  12. Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium baxed semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942
  13. Steinkellner H, Rabot S, Freywald C, Nobis E, Scharf G, Chabicovsky M, Knasmuller S, Kassie F. 2001. Effects of cruciferous vegetables and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutat Res 480-481: 285-297 https://doi.org/10.1016/S0027-5107(01)00188-9
  14. Prochaska HJ, Santamaria AB. 1988. Direct measurement NAD(P)H. Quinone reductase from cells cultured in microtiter wells. A screening assay for anticarcinogenic enzyme inducer. Anal Biochem 169: 328-336 https://doi.org/10.1016/0003-2697(88)90292-8
  15. Prochaska HJ, Santamaria AB. 1988. Direct measurement NAD(P)H. Quinone reductase from cells cultured in microtiter wells. A screening assay for anticarcinogenic enzyme inducer. Anal Biochem 169: 328-336 https://doi.org/10.1016/0003-2697(88)90292-8
  16. Park YJ, Jeon KH, Kim SH, Bae SJ. 2004. The effect on antimicrobial and cytotoxicity of Brassica oleracea L. fractions. J Life Sci 14: 567-572 https://doi.org/10.5352/JLS.2004.14.4.567
  17. Jung BM, Lim SS, Park YJ, Bae SJ. 2005. Inhibitory effects on cell survival and quinone reductase induced activity of Aster yomena fractions on human cancer cells. J Korean Soc Food Sci Nutr 34: 8-12 https://doi.org/10.3746/jkfn.2005.34.1.008
  18. Bae SJ. 2004. Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33: 480-486 https://doi.org/10.3746/jkfn.2004.33.3.480
  19. Bae SJ. 2004. Studies on the antioxidative and antimicrobial effects of Chondria crassicaulis. J Life Sci 14: 411-416 https://doi.org/10.5352/JLS.2004.14.3.411

Cited by

  1. Antimicrobial- and Anticarcinogenic Activities of Amphitrite albicostatu Fractions vol.20, pp.10, 2010, https://doi.org/10.5352/JLS.2010.20.10.1505
  2. Methanol extract of the seaweedGloiopeltis furcata induces G2/M arrest and inhibits cyclooxygenase-2 activity in human hepatocarcinoma HepG2 cells vol.21, pp.1, 2007, https://doi.org/10.1002/ptr.2020
  3. Effect of Growth Inhibition and Quinone Reductase Activity Stimulation of Makgeoly Fractions in Various Cancer Cells vol.37, pp.3, 2008, https://doi.org/10.3746/jkfn.2008.37.3.288
  4. Specific photosynthetic and morphological characteristics allow macroalgae Gloiopeltis furcata (Rhodophyta) to survive in unfavorable conditions vol.52, pp.2, 2014, https://doi.org/10.1007/s11099-014-0026-9
  5. Growth Inhibitory and Quinone Reductase Activity Stimulating Effects of Internal Organs of Aplysia kurodai Fractions on Cancer Cell Lines In vitro vol.20, pp.6, 2010, https://doi.org/10.5352/JLS.2010.20.6.877
  6. Effect of Solvent Extracts from Sargassum hemiphyllum on Inhibition of Growth of Human Cancer Cell Lines and Antioxidant Activity vol.17, pp.11, 2007, https://doi.org/10.5352/JLS.2007.17.11.1533
  7. Inhibitory Effects of Solvent Extracts from Seven Brown Algae on Mutagenicity and Growth of Human Cancer Cells vol.16, pp.7, 2006, https://doi.org/10.5352/JLS.2006.16.7.1080
  8. 김 분획물의 in vitro에서의 항발암효과 vol.34, pp.10, 2005, https://doi.org/10.3746/jkfn.2005.34.10.1514
  9. 참곱슬이(Plocamium telfairiae) 추출물의 암세포 성장억제 효과 vol.16, pp.4, 2006, https://doi.org/10.5352/jls.2006.16.4.659
  10. 말똥성게 분획물에 의한 항 발암 및 항산화 효과 vol.19, pp.5, 2005, https://doi.org/10.5352/jls.2009.19.5.607
  11. 오징어내장 분획물의 in vitro에서의 암세포 성장억제 및 quinone reductase유도 활성 증가 효과 vol.19, pp.9, 2005, https://doi.org/10.5352/jls.2009.19.9.1251
  12. 효소종류에 따른 불등풀가사리 유래 다당류의 이화학적 특성 및 생리활성 vol.24, pp.3, 2005, https://doi.org/10.11002/kjfp.2017.24.3.455
  13. 불등풀가사리 다당류의 분자량에 따른 이화학적 품질특성 및 피부 주름 개선 효과 vol.24, pp.5, 2005, https://doi.org/10.11002/kjfp.2017.24.5.688
  14. The ethanol extract from the rhodophyta Gloiopeltis furcata and its active ingredient docosahexaenoic acid improve exercise performance in mice vol.43, pp.9, 2005, https://doi.org/10.1111/jfbc.12980
  15. PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles vol.19, pp.1, 2019, https://doi.org/10.1186/s12870-019-2125-z
  16. Quality and Antioxidant Activity of Soybean Curd Supplemented with Gloiopeltis furcata Juice vol.33, pp.6, 2021, https://doi.org/10.13000/jfmse.2021.12.33.6.1326