Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-proliferating Effects of Porphyra tenera Fractions on Several Cancer Cell Lines in uitro

김 분획물의 in vitro에서의 항발암효과

  • Shin, Mi-Ok (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Bae, Song-Ja (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
  • 신미옥 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터)
  • Published : 2005.12.01
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Abstract

This study was performed to investigate the effects of Porphyra tenera (PT) on cytotoxicity and quinone reductase (QR) activity in the cancer cells. PT was extracted with methanol and further fractionated into five different types: hexane (PTMH), ethyl-ether (PTMEE), ethylacetate (PTMEA) butanol (PTMB) and aquous (PTMA) partition layers. We determined the cytotoxic effect of these layers on C6, HepG2, MCF-7, and HT-29 cell lines by MTT assay. Among the various fractions, hexane (PTMH) of PT showed the strongest cytotoxic effect on C6, HepG2 and MCF-7 cell lines. PTMH displayed very low level of cytotoxicity at the lower concentration levels and at 300 $\mu$g/mL. PTMH resulted in 87.5$\%$ growth inhibition on C6 cell 70 $\%$ on the HepG2 cell and 89$\%$ on the MCF-7 cell, which were significantly high compared to other fractions. A 400 $\mu$g/mL PTMH concentration level, 99$\%$, 94.5$\%$ and 99$\%$ of cell growth inhibition were resulted on the same cell lines. On HT-29 cell line, both hexane (PTMH) and aqueous (PTMA) fraction of PT showed cytotoxic effects, but the Percentage was not as high as previous results tested on other cell lines such as C6 HepG2 and MCF-7 cell lines. Also, we observed quinone reductase (QR) inducing-effects in all fractions of PT on HepG2 cells. The QR inducing effects of the PTMH on HepG2 cells at 150 $\mu$g/mL concentration was 6.6 times higher than the control. Although further studies are needed, the present work suggests that PT was a potential to be used as a chemopreventive.

본 실험은 홍조해조류의 하나인 김을 메탄올로 추출 후 각 용매별로 다시 분획하여 암세포 성장억제 효과와 QR유도활성 효과 등 생리활성을 연구하였다. 김을 이용하여 4종의 암세포주 C6, HepG2, MCF-7및 HT-29 세포주에 대한 암세포 증식억제 실험을 한 결과 사용한 4종의 암세포주에서 모두 시료첨가 농도에 의존적으로 증식저지 효과가 나타났고, 특히 김의 hexan 분획물에서 가장 높은 암세포 성장 억제효과를 나타내었다. 그리고 본 시료는 신경종양, 간암 및 여성암의 대표적인 유방암세포의 성장억제효과가 탁월하였다. 또한, 사용한 4가지 암세포주중 유일하게 quinone reductase를 가지고 있는 HepG2를 이용한 암 예방지표인 quinone reductase 효소 유도 활성 여부를 측정한 결과 분획물 첨가농도를 50, 100 및 150 g/mL로 첨가하였을 때 PTMH의 첨가농도 50 ${\mu}g/mL$에서 대조군에 비해 약 1.5배 이상의 높은 QR 유도효과를 나타내었고 최종농도 150 ${\mu}g/mL$에서는 약 6.6배의 높은 암 예방 QR 유도효과를 나타내었다.

Keywords

References

  1. Stavric B. 1994. Role of chemopreventiers in human diet. Clin Biochem 27: 319-332 https://doi.org/10.1016/0009-9120(94)00039-5
  2. Reddy L, Odhav B, Bhoola KD. 2003. Natural products for Cancer prevention: a global perspective. Pharmacol Ther 99: 1-13 https://doi.org/10.1016/S0163-7258(03)00042-1
  3. Wynder EL, Gori GB. 1997. Contribution of environment to cancer medicine. J Natl Cancer Inst 58: 826-832
  4. Doll R, Peto R. 1981. The cause of cancer. Quantitative estimates of available risks of cancer in the United States today. J Natl Cancer Inst 66: 1192-1308 https://doi.org/10.1093/jnci/66.6.1192
  5. Banner S, Pastorino U, Lippman SM, Hong WK. 1994. Second international cancer chemoprevention conference. Cancer Res 54: 854-856
  6. Ham SS, Lee SY, Choi M, HwangBo HJ. 1998. Antimutagenicity and cytotoxicity effect of Woorimil wheat flour extracts added with wild herb and seaweed powder. J Korean Soc Food Sci Nutr 27: 1177-1182
  7. Choi HJ, Bae SJ, Kim ND, jung JH, Choi YH. 2004. Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells. Intern J Molec Med 14: 1091-1096
  8. Ha MS, Kim DS, Ryu BH, Ji BH. 1986. Desmutagenic effect of extracts obtained from seaweeds. J Korean Fish Soc 19: 502-508
  9. Ryo T, Hiroko IS, Kaeko H, Saburo H, Susumu H. 1998. Concurrence of agaroid and carrageenan chains in funoran from the red seaweed Gloiopeltis furcata post. et ruprecht. Carbohydrate Polymers 35: 81-87 https://doi.org/10.1016/S0144-8617(97)00230-0
  10. Bae SJ. 2004. Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33: 480-486 https://doi.org/10.3746/jkfn.2004.33.3.480
  11. Park CK, Kang TJ. 2000. The nutritional on functional constituents of laver. Bull Fish Sic Inst, Yosu Nat'l Univ 9: 133-137
  12. Yan X, Nagata T, Fan X. 1998. Antioxidative activities in some common seaweeds. Plants Food Hum Nutr 52: 253-262 https://doi.org/10.1023/A:1008007014659
  13. Lee NH, Oh KL. 2000. Screening of radical scavenging effects from marine algae. Cheju J Life Science 3: 95-101
  14. Lee BH, Choi BW. 1996. Extraction of water soluble antioxidants from seaweeds. J Korean Ind & Eng Chemistry 7: 1069-1077
  15. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbrott BJ, Mayo JG, Shoemaker RH, Boyd MR. 1988. Fesaibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601
  16. Carmichael J. De Graff WG, Gazder AF, Minna JD, Mitchell JB. 1987. Evalution of a tetrazolium baxed semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942
  17. Steinkellner H, Rabot S, Freywald C, Nobis E, Scharf G, Chabicovsky M, Knasmuller S, Kassie F. 2001. Effect of crucifrous vegetable and their constituents on drug metabolizing enzymes involved in the bioactivation of DNAreactive dietary carcinogens. Mutat Res 480-481: 285-297 https://doi.org/10.1016/S0027-5107(01)00188-9
  18. Prochaska HJ, Santamaria AB. 1988. Direct measurement NAD(P)H. Quinone reductase from cells cultured in microtiter wells. A screening assay for anticarcinogenic enzyme inducer. Anal Biochem 169: 328-336 https://doi.org/10.1016/0003-2697(88)90292-8
  19. Park YJ, leon KH, Kim SH, Bae SJ. 2004. The effect on antimicrobial and cytotoxicity of Brassica oleracea L. fractions. J Life Sci 14: 567-572 https://doi.org/10.5352/JLS.2004.14.4.567
  20. Park SY, Joung YH, Shin MO, Joung BM, Bae SJ. 2005. Effect of antimicrobial and cytotoxicity of Undaria pinnatifida Sporophyli fractions. J Korean Soc Food Sci Nutr 34: 765-770 https://doi.org/10.3746/jkfn.2005.34.6.765
  21. Shim SM, Kim MH, Bae SJ. 2001. Cytotoxicity and quinone reductase induced effects of Daucus carota L. leaf extracts on human cancer cells. J Korean Soc Food Sci Nutr 30:86-91
  22. Park YJ, Kim MH, Bae SJ. 2002. Enhancement of anticrcinogenic effect by combination of Lycii fructus with vitamin C. J Korean Soc Food Sci Nutr 31: 143-148 https://doi.org/10.3746/jkfn.2002.31.1.143
  23. Han EJ, Roh SB, Bae SJ. 2000. The effects on quinone reductase induction of Daucus carota L. J Life Sci 10: 79-85
  24. Bae SJ. 2004. Anticarcinogenic effects of Sargassum fulvellum fractions on several human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 33: 480-486 https://doi.org/10.3746/jkfn.2004.33.3.480
  25. Park SY, Jung BM, Choi YH, Bae SJ. 2005. Growth inhibition effects of cell lines by Gloiopeltis furcata fractions in vitro. J Korean Soc Food Sci Nutr 34: 771-775 https://doi.org/10.3746/jkfn.2005.34.6.771
  26. Ryu BH, Kim DS, Cho KJ, Sin DB. 1989. Antitumor activity of seaweeds toward sarcoma-180. Korean J Food Aci Technol 21: 595-600
  27. Park YB, Kim IS, Yoo SJ, Ahn JK, Lee TG, Park DC, Kim SB. 1998. Elucidation of anti-tumor initiator and promoter derived from seaweed-Z; investigation of seaweed extracts suppressing mutagenic activity of PhIP and MeIQx. J Korean Fish Soc 31: 581-586

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