Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antitumor Effect of Young Radish Kimchi Prepared with Young Radish Cultivated in the Soil Containing Sulfur on Sarcoma-180 Tumor Cells Transplanted Mice

유황처리 열무로 제조한 열무김치의 Sarcoma-180 암세포에 대한 고형암 성장 억제효과

  • Kong, Chang-Suk (Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Bak, Soon-Sun (Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Rhee, Sook-Hee (Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Kil, Jeung-Ha (Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Rho, Chi-Woong (Research Center for Exportable Agricultural Crops (RCEC), Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Hwang, Hae-Jun (Research Center for Exportable Agricultural Crops (RCEC), Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Kim, Nak-Ku (Research Center for Exportable Agricultural Crops (RCEC), Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Park, Kun-Young (Research Center for Exportable Agricultural Crops (RCEC), Gyeongsangnam-do Agricultural Research & Extension Services)
  • 공창숙 (부산대학교 식품영양학과 및 김치연구소) ;
  • 박순선 (부산대학교 식품영양학과 및 김치연구소) ;
  • 이숙희 (부산대학교 식품영양학과 및 김치연구소) ;
  • 길정하 (부산대학교 식품영양학과 및 김치연구소) ;
  • 노치웅 (경상남도농업기술원 수출농산물연구소) ;
  • 황해준 (경상남도농업기술원 수출농산물연구소) ;
  • 김낙구 (경상남도농업기술원 수출농산물연구소) ;
  • 박건영 (부산대학교 식품영양학과 및 김치연구소)
  • Published : 2005.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Young radishes (YR, yeolmu in Korean) were cultivated in the soil with and without sulfur. Young radish kimchi-general (YR kimchi-G) was prepared with YR commonly cultivated in the soil without sulfur. Young radish kimchl-sulfur (YR kimchi-S) and young radish kimchi-sulfur with lime mortar (YR kimchi-SL) were prepared with the YR cultivated in the soil with sulfur an4 sulfur added lime mortar on it respectively. Antitumor effects of methanol extracts from the YR kimchis were investigated in sarcoma-180 tumor cell transplanted mice. The solid tumor growth was significantly inhibited by the YR kimchi-SL prepared with YR grown in the soil with sulfur (p < 0.05). The treatment of the methanol extracts from YR kimchi-S and -SL increased the glutathione S-transferase activities and glutathione contents in the livers, compared to thlose of YR kimchi-G and the control. One of the antitumor effects by the YR kimchis was due to the increased the glutathione levels and the glutathione S-transferase activity which is phase 2 enzyme. These results also suggested that the antitumor effects of YR kimchi can be enhanced by using YR cultivated differently in the presence of sulfur that can help to produce sulfur-containing compounds in YR.

유황처리 열무로 제조한 열무김치의 항암효과를 S-180 암세포를 이식한 마우스에 대한 고형암 성장 억제효과를 통하여 검토하였다. 일반 열무 및 유황처리 열무로 제조한 열무김치는 $5^{\circ}C$의 저장온도에서 적숙기 pH 4.3까지 발효시켰다. 열무김치의 메탄올추출물을 이용하여 S-180 cell 이식 마우스에 대한 고형암 성장 억제효과를 관찰한 결과에서 일반 열무김치에 비해 유황처리 열무로 제조한 열무김치를 투여한 군에서 고형암 성장 저해율이 높게 나타났으며, 특히 열무김치 YR kimchi-SL에서 가장 높게 나타났다. GST활성 및 GSH 함량도 유황처리 열무로 제조한 열무김치를 투여한 군에서 가장 높게 나타났다. 따라서 발암상태에서 열무김치추출물은 친전자성 발암물질의 활성 대사산물의 해독작용에 관여하는 GST활성을 증가시켜 독성 물질을 체외로 배설시킴으로서 고형암 성장을 저지하는 역할을 하는 것으로 사료된다. 이는 유황처리에 의해서 열무자체에 항암성 유황 화합물의 농토가 증가하여 높은 항암활성을 나타내었다고 하겠다.

Keywords

References

  1. Korea Institute for Health & Social Affairs. 1989. Food and Nutrient Databases and Dietary Guidance. 5th ed
  2. National Rural Living Science Institute, RDA. 1996. Food composition table. 5th ed
  3. Alegria BC. 1992. Cancer-preventive foods and ingredients. Food Technology 46: 65-68
  4. Kim GE, Lee YS, Kim SH, Cheong HS, Lee JH. 1998. Changes of chlorophyll and their derivative contents during storage of Chinese cabbage, leafy radish and leaf mustard kimchi. J Korean Soc Food Sci Nutr 27: 852-857
  5. Endo Y, Usuki R, Kaneda T. 1984. Prooxidant activities of chlorophylls and their decomposition products on the photoxidation of methyl linoleate. JAOCS 61: 781-784 https://doi.org/10.1007/BF02672135
  6. Endo Y, Usuki R, Kaneda T. 1985. Antioxidant effects of chlorophyll and pheophytin on the autoxidation of oil in the dark, I. Comparison of the inhibitory effects. JAOCS 62: 1375-1378 https://doi.org/10.1007/BF02545962
  7. Endo Y, Usuki R, Kaneda T. 1985. Antioxidant effects of chlorophyll and pheophytin on the autoxidation of oil in the dark, II. The mechanism of antioxidant action chlorophyll effects. JAOCS 62: 1387-1390 https://doi.org/10.1007/BF02545965
  8. Tan YA, Chong CL, Low KS. 1997. Crude palm oil characteristics and chlorophyll content. J Sci Food Agric 75: 281-288 https://doi.org/10.1002/(SICI)1097-0010(199711)75:3<281::AID-JSFA875>3.0.CO;2-A
  9. Gentile JM, Gentile GJ, 1991. The metabolic activation of 4-nitro-o-phenylenediamine by chlorophyll containing plant extracts: The relationship between mutagenicity and anti- mutagenicity. Mutation Res 250: 79-86 https://doi.org/10.1016/0027-5107(91)90164-J
  10. Kim IH. 1989. New Medicine. Kwangjaewon, Seoul. p 80-81
  11. Elizabeth KL, Tracy KS, Rosemary GC, Ian TJ. 2001. Cell death in the colorectal cancer cell line HT29 in response to glucosinolate metabolites. J Sci Food Agric 81: 959-961 https://doi.org/10.1002/jsfa.904
  12. Faukner K, Mithen R, Williamson G. 1998. Selective increase of the potential anticarcinogen 4-methylsulphinyl-butyl glucosinolate in broccoli. Carcinogenesis 19: 605-609 https://doi.org/10.1093/carcin/19.4.605
  13. Fahey JW, Zhang Y, Talalay P. 1997. Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci USA 94: 10367-10372
  14. Dinkova-Kostova AT, Holtzclaw WD, Cole RN, Itoh K, Wakabayashi N, Katoh Y, Yamamoto M, Talalay P. 2002. Direct evidence that sulfhydryl groups of Keapl are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants. Proc Natl Amd Sci USA 99: 11908-11913
  15. Kim KA, Rho CW, Choi KR, Hwang HJ, Choi RS. 2004. Quinone reductase inducer from radish leaf cultivated in the soil containing sulfur. J Korean Soc Food Sci Nutr 33: 946-950 https://doi.org/10.3746/jkfn.2004.33.6.946
  16. Agricultural R&D Promotion Center. 2005. Commercialization and technical development of functional young radish kimchi. 2st Annual Report
  17. Lee YS, Kim DS, Ryu BH, Lee SH. 1992. Antitumor and immunomodulating effects of seaweeds toward sarcoma180 cell. J Korean Soc Food Nutr 21: 544-550
  18. Habig WH, Pabist MJ, Jakoby WB. 1974. Glutathione S-transferase. The first step in mercapturate acid formation. J Biol Chem 249: 7130-7139
  19. Ellaman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-72 https://doi.org/10.1016/0003-9861(59)90090-6
  20. Lowry OH, Rosebrough NH, Farr AL, Randall RJ. 1951. Protein measurement with folin phenol reagent. J Biol Chem 193: 265-275
  21. Choi MW, Kim KY, Park KY. 1997. Effects of kimchi extracts on the growth of sarcoma-180 cells and phagocytic activity of mice. J Korean Soc Food Sci Nutr 26: 254-260
  22. Wim AN, Marinka AB, Martijin HS, Wilbert HM. 1995. Enhancement of rat hepatic and gastointestinal glutathione and glutathione s-transferase by $\alpha$-angelicalactone and flavone. Carcinogenesis 16: 607-612 https://doi.org/10.1093/carcin/16.3.607
  23. Burk RF, Trumble MJ, Lawerence RA. 1980. Rat hepatic cytosolic glutathione dependent enzyme protection against lipid peroxidation in the NADPH -microsomal lipid peroxidation system. Biochem Biophys Acta 618: 35-41 https://doi.org/10.1016/0005-2760(80)90051-X
  24. Meister A, Anderson ME. 1983. Glutathione. Annu Rev Biochem 52: 711-716 https://doi.org/10.1146/annurev.bi.52.070183.003431
  25. Zhang Y, Talalay P, Cho CG, Postner GH. 1992. A major inducer of anticancarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proc Natl Acad Sci USA 89: 2399-2430
  26. Zhang Y, Kensler TW, Cho CG, Postner GH, Talalay P. 1994. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbomyl isothiocyanates. Proc Natl Amd Sci USA 91: 3147-3150
  27. Fahey JW, Haristory X, Dolan PM, Kensler TW, Scholtus I, Stephenson KK, Talalay P, Iozniewski A. 2002. soiforaphane inhibits extracellular, intracellular, antibioticresistant strains of Helicobacter pyroli and prevents benzopyrene-induced stomach tumors. Proc Natl Acad Sci USA 99: 7610-7615

Cited by

  1. A survey of research papers on the health benefits of kimchi and kimchi lactic acid bacteria vol.51, pp.1, 2018, https://doi.org/10.4163/jnh.2018.51.1.1