Nutritional Sciences

The Korean Nutrition Society (한국영양학회)
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The Expressional Changes of Nitric Oxide Synthase in the Rat Brain Following Food Restriction

  • Kang Kyounglan (Research Institute of Medical Nutrition, Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Huh Youngbuhm (Department of Anatomy, College of Medicine, Kyunghee University) ;
  • Park Chan (Department of Anatomy, College of Medicine, Kyunghee University) ;
  • Choue Ryo Won (Research Institute of Medical Nutrition, Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyunghee University)
  • Published : 2005.11.01
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Abstract

This study investigated the changes in the neuronal nitric oxide synthase (nNOS) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activities during food restriction in the rat brain such as cerebral cortex, cerebellum, caudate pautamen and hypothalamus. The rats were placed on a restricted feeding schedule consisting of half the ad libitum quantity for 3 days and 1, 2, 4, 6 and 9 weeks, and a free feeding schedule for 4 weeks. The loss of body weight peaked after 1 week of food restriction and persisted during the entire 9-week period of food restriction. The dramatic weight change in the first week ($12\%$) and the reduction in weight changes thereafter suggest that major adaptation changes occur early and body maintenance occurs subsequently. In the hypothalamus, the optical densities of the NADPH-d and nNOS immunoreactivities were found to be significantly higher in the 1-week and lower in the 9-week food restricted group than in the ad libitum fed control rats. In contrast, in the cerebral cortex, the optical densities of the NADPH-d- and nNOS-positive neurons were not changed significantly during the period of food restriction. This study provides the morphological evidence showing that food restriction has a significant effect on the nitric oxide synthesizing system of the hypothalamus.

Keywords

References

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