Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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RT-PCR of Up-Regulated Factors in Abnormally Proliferated Vascular Endothelial Cells by 1, 2-Dimethylhydrazine.

DMH(1,2-Dimethylhydrazine)에 의해 비정상적으로 증식된 혈관 내피세포에서 발현증가 인자들에 대한 RT-PCR의 결과

  • Kim, Sung-Ho (Ha-Neul Aesthetic Clinic) ;
  • Kang, Young-Seok (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University) ;
  • Bae, Yong-Chan (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University) ;
  • Park, Suk-Young (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University) ;
  • Nam, Su-Bong (Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University)
  • 김성호 (하늘성형외과) ;
  • 강영석 (부산대학교 의과대학 성형외과학교실) ;
  • 배용찬 (부산대학교 의과대학 성형외과학교실) ;
  • 박숙영 (부산대학교 의과대학 성형외과학교실) ;
  • 남수봉 (부산대학교 의과대학 성형외과학교실)
  • Received : 2005.06.01
  • Published : 2005.11.10
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Abstract

Many studies for verifying angiogenesis have been in progress, especially in the field of abnormal vascular proliferation to explain the pathogenesis and to develop a treatment of several diseases. In our previous experiments, endothelial cell proliferations were induced by DMH stimulation in vitro, and the 177 factors(142 up-regulated and 35 down-regulated factors) were identified. Among the up-regulated factors, 9 substances (EFEMP1, CTGF, CYR61, $ITG{\beta}1$, FHL2, SERPINE1, MYC, PTTG1 and MSH6) were selected, which were related to cell proliferation and showed high signal intensities. The RNA was isolated from HUVECs at the time of 0, 6, 12, 24 hours after the DMH treatment, and RNA of control group HUVECs was also isolated. Genetic information of selected molecules was used to make primer for each, and RT-PCR was performed to analyze both groups. In control and treatment groups, each substance presented variety of manifestation degree according to time differences. EFEMP1, CTGF, CYR61, $ITG{\beta}1$, FHL2 and MYC were related to abnormal vascular proliferation steadily and SERPINE1, PTTG1 and MSH6 were related secondarily. CTGF was related to both normal and abnormal proliferation, but it played a more significant role in abnormal proliferation from earlier stage. EFEMP1, CYR61, $ITG{\beta}1$, FHL2 and MYC were similar to CTGF, although the relation appeared lately. Further study should be performed to analyze the expressions and the interactions of growth factors, which could be utilized in the new therapeutic development.

Keywords

References

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