DOI QR코드

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Newly Developed Microsatellite Markers of Mystus nemurus Tested for Cross-Species Amplification in Two Distantly Related Aquacultured Catfish Species

  • Chan, S.C. (Department of Biology, Faculty of Science, Universiti Putra Malaysia) ;
  • Tan, S.G. (Department of Biology, Faculty of Science, Universiti Putra Malaysia) ;
  • Siraj, S.S. (Department of Biology, Faculty of Science, Universiti Putra Malaysia) ;
  • Yusoff, K. (Department of Microbiology, Faculty of Biotechnology and Biomolecular)
  • 투고 : 2004.10.11
  • 심사 : 2005.05.01
  • 발행 : 2005.11.01

초록

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.

키워드

참고문헌

  1. Ashley, M. V. and B. D. Dow. 1994. The use of microsatellite analysis in population biology: Background, methods and potential applications. In Molecular Ecology and Evolution: Approaches and Applications. (Ed. B. Schierwater, B. Streit, G. P. Wagner and R. DeSalle). Switzerland: Bikhauser Verlag, pp. 185-201.
  2. Baratti, M., A. Alberti, M. Groenen, T. Veenendaal and F. D. Fulgheri. 2001. Polymorphic microsatellites developed by cross-species amplification in common pheasant breeds. Anim. Genet. 32: 222-225.
  3. Fisher, P. J., R. C. Gardner and T. E. Richardson. 1996. Single locus microsatellites isolated by 5’ anchored PCR. Nucleic Acids Research 24:4369-4371.
  4. FitzSimmons, N. N., C. Moritz and S. S. Moore. 1995. Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Molecular Biology and Evolution 12:432-440.
  5. Hempel, K. and R. Peakall. 2003. Cross-species amplification from crop soybean Glycine max provides informative microsatellite markers for the study of inbreeding wild relatives. Genome 46:382-393.
  6. Koskinen, M. T. and C. R. Primmer. 1999. Cross-species amplification of salmonid microsatellites which reveal polymorphism in European and Arctic grayling, Salmonidae: Thymallus spp. Hereditas 131:171-176.
  7. Rico, C., I. Rico and G. Hewitt. 1996. 470 million years of conservation of microsatellite loci among fish species. Proceeding of Royal Society of London in Biology Sciences 263:549-557.
  8. Rozen, S. and H. J. Skaletsky. 1997. Primer 3. http://wwwgenome. wi.mit.edu/cgi-bin/primer/prime$r_3$_www.cgi.
  9. Sun, W., H. Chang, Z. J. Ren, R. Q. Geng, S. X. Lu, L. Du and K. Tsunoda. 2004. Genetic differentiation between Sheep and Goats based on Microsatellite DNA. Asian-Aust. J. Anim. Sci. 17(5):583-587.
  10. Taggart, J. B., R. A. Hynes, P. A. Prodohl and A. Ferguson. 1992. A simplified protocol of routine total DNA isolation from salmonid fishes. J. Fish Biol. 40:963-965.
  11. Yeh, F. C. and T. J. B. Boyle. 1997. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany 129:157.