Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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The Comparative Study between PLGA and Chitosan Scaffolds for Cartilage Tissue Engineering

연골조직공학에서 Polyactic-Glycolic Acid와 Chitosan 골격의 비교

  • Lee, Yong Jik (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital) ;
  • Chung, Ho Yun (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital) ;
  • Shin, Dong Phil (Dr. Shin's Aesthetic Plastic Surgical Clinic) ;
  • Kim, Jong Yeop (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital) ;
  • Yang, Jung Duk (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital) ;
  • Lee, Dong Gul (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital) ;
  • Park, Jae Woo (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital) ;
  • Cho, Byung Chae (Department of Plastic & Reconstructive Surgery, Kyungpook National University Hospital) ;
  • Baik, Bong Soo (Department of Plastic & Reconstructive Surgery, Ulsan Dongkang Hospital)
  • 이용직 (경북대학교 의과대학 성형외과학교실) ;
  • 정호윤 (경북대학교 의과대학 성형외과학교실) ;
  • 신동필 (신동필 성형외과) ;
  • 김종엽 (경북대학교 의과대학 성형외과학교실) ;
  • 양정덕 (경북대학교 의과대학 성형외과학교실) ;
  • 이동걸 (경북대학교 의과대학 성형외과학교실) ;
  • 박재우 (경북대학교 의과대학 성형외과학교실) ;
  • 조병채 (경북대학교 의과대학 성형외과학교실) ;
  • 백봉수 (울산동강병원 성형외과)
  • Received : 2005.04.25
  • Published : 2005.09.10
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Abstract

Clinical application of the cartilage formed by tissue engineering is of no practical use due to the failure of long-term structural integrity maintenance. One of the important factors for integrity maintenance is the biomaterial for a scaffold. The purpose of this study is to evaluate the difference between polylactic-co-glycolic acids (PLGA) and chitosan as scaffolds. Human auricular chondrocytes were isolated, cultured, and seeded on the scaffolds, which were implanted in the back of nude mice. Eight animals were sacrificed at 4, 8, 12, 16, and 24 weeks after implantation respectively. In gross examination and histological findings, the volume of chondrocyte-PLGA complexes was decreased rapidly. The volume of chondrocyte-chitosan complexes was well maintained with a slow decrease rate. The expression of type II collagen protein detected by immunohistochemistry and western blots became weaker with time in the chondrocyte-PLGA complexes. However, the expression in the chondrocyte-chitosan complexes was strong for the whole period. Collagen type II gene expressions using RT-PCR showed a similar pattern. In conclusion, these results suggest that chitosan is a superior scaffold in cartilage tissue engineering in terms of structural integrity maintenance. It is expected that chitosan scaffold may become one of the most useful scaffolds for cartilage tissue engineering.

Keywords

Acknowledgement

Supported by : 경북대학교

References

  1. Zalzal GH, Cotton RT, McAdams AJ: Cartilage grafts-present status. Head Neck Surg 8: 363, 1986 https://doi.org/10.1002/hed.2890080507
  2. Rodriguez A, Cao YL, Ibarra C, Pap S, Vacanti M, Eavery RD, Vacanti CA: Characteristics of cartilage engineered from human pediatric auricular cartilage. Plast Reconstr Surg 103: 1111, 1999 https://doi.org/10.1097/00006534-199904040-00001
  3. Chalain TD, Philips JH, Hinek A: Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and x-elastin. J Biomed Mater Res 44: 280, 1999 https://doi.org/10.1002/(SICI)1097-4636(19990305)44:3<280::AID-JBM6>3.0.CO;2-H
  4. Risbud MV, Sittinger M: Tissue engineering: advances in vitro cartilage generation. Trends Biotechnol 20: 351, 2002 https://doi.org/10.1016/S0167-7799(02)02016-4
  5. Vacanti CA, Langer R, Schloo B, Vacanti JP: Synthetic polymers seeded with chondrocytes provide a template for new cartilage formation. Plast Reconstr Surg 88: 753, 1991 https://doi.org/10.1097/00006534-199111000-00001
  6. Kim WS, Vacanti JP, Cima L, Mooney D, Upton J, Puelacher WC, Vacanti CA: Cartilage engineered in predetermined shapes employing cell transplantation on synthetic biodegradable polymers. Plast Reconstr Surg 94: 233, 1994 https://doi.org/10.1097/00006534-199408000-00001
  7. Bahrami S, Stratmann U, Wiesmann HP, Mokrys K, Bruckner P, Szuwart T: Periosteally derived osteoblast-like cells differentiate into chondrocytes in suspension culture in agarose. Anat Rec 259: 124, 2000 https://doi.org/10.1002/(SICI)1097-0185(20000601)259:2<124::AID-AR2>3.0.CO;2-O
  8. Ma HL, Hung SC, Lin SY, Chen YL, Lo WH: Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads. J Biomed Mater Res 64A: 273, 2003 https://doi.org/10.1002/jbm.a.10370
  9. Vacanti JP: Beyond transplantation. Arch Surg 123: 545, 1988 https://doi.org/10.1001/archsurg.1988.01400290027003
  10. Cao Y, Vacanti JP, Paige KT, Upton J, Vacanti CA: Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear. Plast Reconstr Surg 100: 297, 1997 https://doi.org/10.1097/00006534-199708000-00001
  11. Saadeh PB, Brent B, Mehrara BJ, Steinbrech DS, Ting V, Gittes GK, Longaker MT: Human cartilage engineering: chondrocyte extraction, proliferation, and characterization for construct development. Ann Plast Surg 42: 509, 1999 https://doi.org/10.1097/00000637-199905000-00008
  12. Dessau W, Sasse J, Timpl R, Jilek F, von der Mark K: Synthesis and extracellular deposition of fibronectin in chondrocyte cultures. J Cell Biol 79: 342, 1978 https://doi.org/10.1083/jcb.79.2.342
  13. Glowacki J, Trepman E, Folkman J: Cell shape and phenotypic expression in chondroeytes. Proc Soc Exp Biol Med 172: 93, 1983
  14. Tomihata K, Ikada Y: In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. Biomaterials 18: 567, 1997 https://doi.org/10.1016/S0142-9612(96)00167-6