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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Peptide Domain Involved in the Interaction between Membrane Protein and Nucleocapsid Protein of SARS-associated Coronavirus

  • Fang, Xiaonan (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Ye, Linbai (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Timani, Khalid Amine (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Li, Shanshan (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Zen, Yingchun (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Zhao, Meng (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Zheng, Hong (State Key Laboratory of Virology, College of Life Science, Wuhan University) ;
  • Wu, Zhenghui (State Key Laboratory of Virology, College of Life Science, Wuhan University)
  • Published : 2005.07.31
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Abstract

Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus (CoV) that was identified and molecularly characterized in 2003. Previous studies on various coronaviruses indicate that protein-protein interactions amongst various coronavirus proteins are critical for viral assembly and morphogenesis. It is necessary to elucidate the molecular mechanism of SARS-CoV replication and rationalize the anti-SARS therapeutic intervention. In this study, we employed an in vitro GST pull-down assay to investigate the interaction between the membrane (M) and the nucleocapsid (N) proteins. Our results show that the interaction between the M and N proteins does take place in vitro. Moreover, we provide an evidence that 12 amino acids domain (194-205) in the M protein is responsible for binding to N protein. Our work will help shed light on the molecular mechanism of the virus assembly and provide valuable information pertaining to rationalization of future anti-viral strategies.

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References

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