Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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The Inhibitory Effect of Zinc on the Cadmium- induced Apoptosis in Human Breast Cancer Cells

유방암세포에서 카드뮴에 의해 유도된 아폼토시스에 대한 아연의 저해 효과

  • Oh Ji Young (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Lee Su Jung (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Shin Jae Ho (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim Tae Sung (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Moon Hyun Ju (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kang Il Hyun (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kang Tae Seok (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim An Keun (College of Pharmacy, Sookmyung Women's University) ;
  • Han Soon Young (Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Administration)
  • 오지영 (국립독성연구원 내분비장애물질팀) ;
  • 이수정 (국립독성연구원 내분비장애물질팀) ;
  • 신재호 (국립독성연구원 내분비장애물질팀) ;
  • 김태성 (국립독성연구원 내분비장애물질팀) ;
  • 문현주 (국립독성연구원 내분비장애물질팀) ;
  • 강일현 (국립독성연구원 내분비장애물질팀) ;
  • 강태석 (국립독성연구원 내분비장애물질팀) ;
  • 김안근 (숙명여자대학교 약학대학) ;
  • 한순영 (국립독성연구원 내분비장애물질팀)
  • Published : 2005.12.01
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Abstract

아연은 다양한 독성 물질로부터 유도된 아폼토시스를 저해하는 것으로 알려져 있으나 이 기전에 대해서는 명확히 밝혀지지 않았다. 본 연구에서는 인간 유방암 세포 MCF-7에 카드뮴을 처리하였을 때 유도되는 아폼토시스에 대한 아연의 저해효과를 살펴보았다. 아연의 아폼토시스 저해 효과는 DNA분절현상, 핵의 쪼개짐 그리고 caspase-9의 발현을 통하여 확인하였다. 또한 아연의 아폼토시스 저해효과가 카드뮴에 의한 산화적 스트레스와 관련이 있는지 확인하기 위하여 활성산소인 peroxide의 농도를 세포내에서 측정하였다. 나아가 superoxide dismutase (SOD), catalase (CAT) 그리고 glutahion redurtase (CR)같은 활성산소에 대한 인체내 방어기작으로 작용하는 항산화 효소의 활성을 측정하였다. 본 연구를 통해 아연이 카드뮴에 의해 생성된 세포내의 활성산소의 양을 감소시키고 항산화 효소를 회복시키는 기전이 카드뮴에 의한 아폼토시스를 저해하는 한 요인으로 사료되어진다.

Keywords

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