Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimization of Chitosan-Alginate Encapsulation Process Using Pig Hepatocytes or Development of Bioartificial Liver

  • LEE , JI-HYUN (Samsung Biomedical Research Institute) ;
  • LEE, DOO-HOON (Department of Chemical Engineering, Dongguk University) ;
  • SON, JEONG-HWA (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • PARK, JUNG-KEUG (Department of Chemical Engineering, Dongguk University) ;
  • KIM, SUNG-KOO (Department of Biotechnology and Bioengineering, Pukyong National University)
  • Published : 2005.02.01
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Abstract

Chitosan-alginate capsules were formed by electrostatic interactions and exhibited an appropriate mechanical strength, permeability, and stability for the culture of hepatocytes. Pig hepatocytes were isolated and hepatocyte spheroids formed and immobilized in chitosan-alginate capsules. An encapsulation procedure of 3 min and spheroid formation period of 24 h were the optimum conditions for the best liver functions. Pig hepatocytes with a cell density of $6.0{\tomes}10^6$ cells/ml in the capsules were found to be most suitable for application in a bioartificial liver support system. The encapsulated pig hepatocyte spheroids exhibited stable ammonia removal and urea secretion rates in a bioreactor for 2 weeks. Accordingly, chitosan-alginate encapsulated hepatocyte spheroids in a packed-bed bioreactor would appear to have potential as a bioartificial liver.

Keywords

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