Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Suppression of nitric oxide and interleukin-6 production by methanol extract of Sophorae Flos in macrophage cells

괴화 추출물이 대식세포에서의 nitric oxide와 interleukin-6의 생성에 미치는 영향

  • Lee, Ji-Eun (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Lee, Ju-Youn (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Choi, Jeom-Il (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Kim, Sung-Jo (Department of Periodontology, College of Dentistry, Pusan National University)
  • 이지은 (부산대학교 치과대학 치주과학교실) ;
  • 이주연 (부산대학교 치과대학 치주과학교실) ;
  • 최점일 (부산대학교 치과대학 치주과학교실) ;
  • 김종관 (연세대학교 치과대학 치주과학교실) ;
  • 김성조 (부산대학교 치과대학 치주과학교실)
  • Published : 2005.03.30
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Abstract

Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sopharae Flos was sliced, and extracted with 100% methanol. LPS from p. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supematants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAw264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAw264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.

Keywords

References

  1. Moncada S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathology, and pharmacology. Pharmacol Rev 1991;43:109-142
  2. Nathan C, Xie QW. Nitric oxide synthases: roles, tolls and controls. Cell 1994;78:915-918 https://doi.org/10.1016/0092-8674(94)90266-6
  3. Geller DA, Nussler AK, Di Silvio M et al, Cytokines, endotoxin, and glucocorticoids regulate the expression of inducible nitric oxide synthase in hepatocytes. Proc Natl Acad Sci USA 1993;90:522-526 https://doi.org/10.1073/pnas.90.2.522
  4. Nathan C, Xie QW. Regulation of biosynthesis of nitric oxide. J Biol Chem 1994;269:13725-13728
  5. Southey A, Tanaka S, Murakami T et al, Pathophysiological role of nitric oxide in rat experimental colitis. Int J lmmunopharmacol 1997;19:669-676 https://doi.org/10.1016/S0192-0561(97)00002-7
  6. Weinberg JB, Granger DL, Pisetsky DS et al, The role of nitric oxide in the pathogenesis of spontaneous murine autoimmune disease: increased nitric oxide production and nitric oxide synthase expression in MRL-lpr/lpr mice, and reduction of spontaneous glomerulonephritis and arthritis by orally administered NG-monomethyl-L-arginine. J Exp Med 1994;179:651-660 https://doi.org/10.1084/jem.179.2.651
  7. Matejka M, Partyka L, Ulm C, Solar P, Sinzinger H. Nitric oxide synthesis is increased in periodontal disease. J Periodont Res 1998;33:517-518
  8. Blix IJ, Helgeland K. LPS from Actinobacillus actinomycetemcomitans and production of nitric oxide in murine macrophages J774. Eur J Oral Sci 1998;106:576-581 https://doi.org/10.1046/j.0909-8836.1998.eos106107.x
  9. Sosroseno W, Barid I, Herminajeng E, Susilowati H. Nitric oxide production by a murine macrophage cell line (RA w264. 7) stimulated with lipopolysaccharide from Actinobacillus actinomycetemcomitans. Oral Microbiol Immunol 2002;17:72-78 https://doi.org/10.1046/j.0902-0055.2001.00091.x
  10. Kim SJ, Ha MS, Choi EY, Choi JI, Choi IS. Prevotellz intermedia lipopolysaccharide stimulates release of nitric oxide by inducing expression of inducible nitric oxide synthase. J Periodont Res 2004;39;424-431
  11. Kim SJ, Ha MS, Choi EY, Choi JI, Choi IS. Nitric oxide production and inducible nitric oxide synthase expression induced by Prevotell nigrescens lipopolysaccharide. FEMS Immunol Med Microbiol. 2005;43:51-58 https://doi.org/10.1016/j.femsim.2004.07.001
  12. Batista AC, Silva TA, Chun JH, Lara VS. Nitric oxide synthesis and severity of human periodontal disease. Oral Diseases 2002;8:254-260 https://doi.org/10.1034/j.1601-0825.2002.02852.x
  13. Hirose M, Ishihara K, Saito A et al, Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue. J Periodontol 2001;72:590-597 https://doi.org/10.1902/jop.2001.72.5.590
  14. Kendall HK, Haase HR, Li H, Xiao Y, Bartold PM. Nitric oxide synthase type-II is synthesized by human gingival tissue and cultured human gingival fibroblasts. J Periodont Res 2000;35:194-200 https://doi.org/10.1034/j.1600-0765.2000.035004194.x
  15. Lappin DF, Kjeldsen M, Sander L, Kinane DF. Inducible nitric oxide synthase expression in periodontitis. J Periodont Res 2000;35:369-373
  16. Kishimoto T. The biology of interleukin-6. Blood 1989;74:1-10
  17. Hirano T, Akira S, Taga T, Kishimoto T. Biological and clinical aspects of interleukin-6. Immunol Today 1990;11;443-449
  18. Ishimi Y, Miyaura C, Jin CH et al, IL-6 is produced by osteoblasts and induce bone resorption. J Immuol 1990;145:3897-3903
  19. Takahashi K, Takashiba S, Nagai A et al. Assessment of interleukin-6 in the pathogenesis of periodontal disease. J Periodontol 1994;65:147-153 https://doi.org/10.1902/jop.1994.65.2.147
  20. Guillot JL, Pollock SM, Johnson RB. Gingival interleukin-6 concentration following phase 1 therapy. J Periodontol 1990;66:667-672
  21. Takada H, Mihara J, Morisaki I, Hamada S. Production of cytokines by human gingival fibroblast, 1991:265-276. In S Hamada, SC Holt, and JR McGhee (ed.), Periodontal disease: pathogenesis and host immune response. Quintessence, Tokyo
  22. Westphal O, Jann K. Bacterial lipopolysaccharides: extraction with phenol-waterand further applications of the procedure. In: RL Whistler eds, Methods in carbohydrate chemistry. New York: Academic Press, 1965:83-91
  23. Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978;87:206-210 https://doi.org/10.1016/0003-2697(78)90586-9
  24. Green LC, Wagner DA, Glogowski J et al. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 1982;126:131-138 https://doi.org/10.1016/0003-2697(82)90118-X
  25. Rappolee DA, Wang A, Mark D, Werb Z. Novel method for studying mRNA phenotypes in single or small numbers of cells. J Cell Biochem 1989;39:1-11.
  26. Goodson JM, Tanner A. Antibiotic resistance of the subgingival microbiota following local tetracycline therapy. Oral Microbiol Immunol 1992;7:113-117 https://doi.org/10.1111/j.1399-302X.1992.tb00520.x
  27. Lang NP, Hotz P, Graf H et al Effects of supervised chlorhexidine mouthrinses in children. A longitudinal clinical trial. J Periodontal Res. 1982;17:101-111 https://doi.org/10.1111/j.1600-0765.1982.tb01135.x
  28. Loe H. Does chlorhexidine have a place in the prophylaxis of dental diseases? J Periodontal Res Suppl. 1973;12:93-99
  29. Eriksen HM, Nordbo H, Kantanen H, Ellingsen JE. Chemical plaque control and extrinsic tooth discoloration. J Clin Periodontol 1985;12:345-350 https://doi.org/10.1111/j.1600-051X.1985.tb00924.x
  30. Vogel RI, Schneider L, Goteiner D. The effects of a topically-active nonsteroidal anti-inflammatory drug on ligature-induced periodontal disease in the squirrel monkey. J Clin Periodontol 1986;13:139-144
  31. Heasman PA, Seymour RA, Boston PF. The effect of a topical non-steroidal anti-inflammatory drug on the development of experimental gingivitis in man. J Clin Periodontol 1989;16:353-358
  32. Williams RC, Jeffcoat MK, Howell TH et at. Altering the progression of human alveolar bone loss with the non-steroidal anti-inflammatory drug flurbiprofen. J Periodontol 1989;60: 485-490
  33. Williams RC, Jeffcoat MK, Howell TH et at. Topical flurbiprofen treatment of periodontitis in beagles J Periodont Res 1988;23:166-169
  34. Howell TH, Jeffcoat MK, Goldhaber P et at. Inhibition of alveolar bone loss in beagles with the NSAID naproxen. J Periodont Res 1991;26:498-501 https://doi.org/10.1111/j.1600-0765.1991.tb01801.x
  35. Bjamason I, Hayllar J, MacPherson AJ, Russell AS. Side effects of nonsteroidal anti-inflammatory drugs on the small and large intestine in humans. Gastroenterol 1993;104:1832-1847
  36. Sikes DH, Agrawal NM, Zhao WW et al, Incidence of gastroduodenal ulcers associated with valecoxib compared with that of ibuprofen and diclofenac in patients with osteoarthritis. Eur J Gastroenterol Hepatol 2002;14:1101-1111 https://doi.org/10.1097/00042737-200210000-00011
  37. 생약학연구회. 현대생약학, 학창사 1994:317-321