Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival and periodontal ligament fibroblasts treated with lipopolysaccharide from Prevotella intermedia

Prevotella intermedia의 세균내독소가 치은섬유아세포와 치주인대세포에서의 matrix metalloproteinase 및 tissue inhibitor of metalloproteinase의 발현에 미치는 영향

  • Kim, Sung-Jo (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Choi, Eun-Young (Department of Life Science, College of Natural Science, Silla University) ;
  • Choi, In-Soon (Department of Life Science, College of Natural Science, Silla University) ;
  • Lee, Ju-Youn (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Choi, Jeom-Il (Department of Periodontology, College of Dentistry, Pusan National University) ;
  • Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University)
  • 김성조 (부산대학교 치과대학 치주과학교실) ;
  • 최은영 (신라대학교 자연대학 생명과학과) ;
  • 최인순 (신라대학교 자연대학 생명과학과) ;
  • 이주연 (부산대학교 치과대학 치주과학교실) ;
  • 최점일 (부산대학교 치과대학 치주과학교실) ;
  • 김종관 (연세대학교 치과대학 치주과학교실)
  • Published : 2005.03.30
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Abstract

Matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes and implicated in the remodeling and degradation of extracellular matrix under both physiological and pathological conditions. Connective tissue degradation in periodontal diseases is thought to be due to excessive MMP activities over their specific inhibitors. The effects of lipopolysaccharide (LPS) from Prevotella intermedia, one of the major putative pathogens of periodontitis, on the expression of mRNA for MMPs and tissue inhibitors of metalloproteinases (TIMPs) in human gingival and periodontal ligament fibroblasts were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of mRNAs encoding MMP-1, -2, -3, -10, and -14 was increased in human gingival fibroblasts treated with p. intermedia LPS, whereas MMP-11 and TIMP-2 mRNA expression was decreased in these cells stimulated with LPS. P. intermedia LPS increased the MMP-1, -2, -10, -11, and -14 mRNA expression and decreased TIMP-1 and -2 mRNA expression in human periodontal ligament fibroblasts. These findings imply that P. intermedia LPS may play an important role in the connective tissue degradation in periodontitis.

Keywords

References

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