Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effect of the Physiologically Active Compounds in Phlomidis Radix on Cell Cycle Regulation in Human Gingival Fibroblasts

속단의 생리활성성분이 치은섬유아세포의 세포주기조절에 미치는 영향

  • You, Suk-Joo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Jang, Kil-Young (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Yoon, Ho-Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Choi, Ho-Chul (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Sung, Ki-Jong (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Hyun-A (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Pi, Sung-Hee (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 유석주 (원광대학교 치과대학 치주과학교실) ;
  • 장길용 (원광대학교 치과대학 치주과학교실) ;
  • 윤호상 (원광대학교 치과대학 치주과학교실) ;
  • 최호철 (원광대학교 치과대학 치주과학교실) ;
  • 선기종 (원광대학교 치과대학 치주과학교실) ;
  • 김현아 (원광대학교 치과대학 치주과학교실) ;
  • 피성희 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실)
  • Published : 2005.03.30
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Abstract

The purpose of present study was to investigate the effects of physiologically active compound (SD62-122) from Phlomidis Radix on the cell cycle progression and its molecular mechanism in human gingival fibroblasts(HGFs). For this purpose, fibroblasts were isolated and cultured from excisioned gingiva during crown lengthening procedure in healthy adult. The following parameter were evaluated that there are cell number counting, MIT assay, cell cycle progression, western blot analysis. The cell number and MIT assay of primary cultured fibroblast was not increased at 2 days but significant increased compare to negative control at 3days(p<0.05). S phase was increased and G1 phase decreased in both $10^{-8}M$ and $10^{-9}M$ of SD62-122 in cell cycle analysis. The cell cycle regulation protein levels of Cyclin $D_1$, Cyclin E, cdk 2, cdk 4 and cdk 6 were increased compare to control in both $10^{-8}M$ and $10^{-9}M$ of SD62-122. The protein levels of p21 and p53 were decreased compare to control, but the level of pRb was not changed compare to control in $10^{-9}M$ of SD2-122. These results suggested that physiologically active compound (SD62-122) isolated from Phlomidis Radix increases the cell proliferation and cell cycle progression in HGFs, which is linked to increased cell cycle regulation protein levels of Cyclin $D_1$, Cyclin E, cdk 2, cdk 4 and cdk 6, and decreased the levels of p21, p53.

Keywords

References

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